Eradication of Extensive Lymph-Node, Bone and Pleural Metastases of a Breast-Cancer Patient Treated With Radiation, Immunotherapy and Oral Recombinant Methioninase.

Background/aim: Metastatic breast cancer is a recalcitrant disease with a poor prognosis. Novel targets and therapies are necessary to improve the survival rate of patients with metastatic breast cancer. Previous pre-clinical and clinical studies, have demonstrated the effectiveness of oral recombinant methioninase (o-rMETase) against breast cancer.

Limited predictive value of pretreatment P-glycoprotein immunostaining for chemotherapy efficacy and survival in long-follow-up osteosarcoma patients.

The clinical utility of P-glycoprotein (P-gp) in osteosarcoma remains controversial. This retrospective cohort study aimed to investigate the association between P-gp expression and clinicopathological factors, chemotherapy (CTx) response, and prognosis in osteosarcoma. Twenty-three osteosarcoma patients treated at our institution between 2007 and 2013 were included.

Molecular and genetic evidence for the role of AMBRA1 in suppressing S-phase entry and tumorigenesis.

AMBRA1, which was initially reported to be essential for nervous system development via autophagy and cell proliferation control, also functions as a tumor suppressor by regulating the ubiquitination of D-type cyclins through interaction with DDB1-Cullin4A/4 B E3 ligase. We had identified a missense mutation in through exome analysis of a family with Cowden syndrome. The patient-type mutant showed reduced DDB1 binding and impaired cyclin D degradation.

Mortality following fragility fractures of the pelvis: Systematic review and meta-analysis.

Background: Fragility fractures of the pelvis (FFPs) in older adults are increasingly recognized as serious injuries with substantial morbidity. However, mortality after FFP has not been comprehensively quantified across timepoints or patient subgroups, limiting the ability to inform prognosis and guide clinical strategies. This systematic review and meta-analysis aimed to assess the 1-year mortality following FFP in patients aged ≥60 years.

Research mapping of trends in conservative management and outcomes of fragility fractures of the Pelvis.

Background: Fragility fractures of the pelvis (FFP) pose significant challenges in geriatric care, with conservative management strategies remaining inconsistent. This scoping review aimed to map current trends in conservative treatment strategies for FFP and summarize associated clinical outcomes and complications.

Methods: We examined (1) the types of conservative treatments used, (2) their temporal changes, and (3) their associated clinical outcomes.

The Combination of the Autophagy Inhibitor Chloroquine and Recombinant Methioninase Has Selective Synergistic Efficacy on Human Colon Cancer Cells But Not on Normal Human Fibroblasts.

Background/aim: Metastatic colon cancer is a recalcitrant disease with a 5-year survival rate of 15.7-26.0%, and more effective treatments are necessary.

Prognostic factors and the impact of neoadjuvant chemotherapy on the development of metastasis in patients with undifferentiated pleomorphic sarcoma of soft tissue.

Background: Although undifferentiated pleomorphic sarcoma (UPS), previously termed as malignant fibrous histiocytoma (MFH), is one of the most common soft tissue sarcomas, the number of studies focusing on UPS remains limited. This retrospective study aimed to identify prognostic factors and assess the clinical impact of chemotherapy on the development of metastasis in patients with UPS.

Methods: This study included 147 patients with UPS who underwent tumor resection.

A perspective on patient-derived orthotopic xenograft (PDOX) mouse models for identification of novel and individualized treatment for sarcoma.

Sarcomas, including osteosarcoma and soft tissue sarcoma, are heterogeneous and rare diseases with limited treatment options and a high metastatic potential. Despite advancements in immunotherapy and targeted therapies, many sarcoma patients have limited durable responses to these treatments. Therefore, individualized precision medicine and novel drug discovery are greatly needed.

Selective Synergy of Ivermectin Combined With Recombinant Methioninase Against Colon-Cancer Cells in Contrast to Normal Fibroblasts.

Background/aim: Recent studies have shown that ivermectin, developed as an anti-parasitic drug, has efficacy against several cancer types. Methionine restriction, including the use of recombinant methioninase (rMETase), has been developed to target methionine addiction, a fundamental hallmark of cancer, termed the Hoffman effect. Metastatic colorectal cancer (CRC) is a recalcitrant disease that requires novel and disruptive treatment approaches.

Current and emerging systemic treatment options for malignant fibrous histiocytoma/undifferentiated pleomorphic sarcoma.

Undifferentiated pleomorphic sarcoma (UPS)/malignant fibrous histiocytoma (MFH) is the second most common soft-tissue sarcoma. The standard treatment options for UPS/MFH include tumor excision with appropriate surgical margins, radiation therapy, and chemotherapy. Preferable clinical outcomes can be expected in patients with resectable disease, whereas the clinical outcomes in patients with metastatic disease are unsatisfactory despite multidisciplinary treatment.

Insulin-like growth factor 1 protects cochlear outer hair cells against cisplatin.

Cisplatin, an effective anti-neoplastic drug widely used in oncology protocols, has an adverse effect such as ototoxicity, for which no current treatment exists. Histological lesions in the cochlea after cisplatin administration are most prominent in outer hair cells in the organ of Corti. We have previously reported that insulin-like growth factor 1 (IGF1) protects cochlear hair cells (HCs) against several types of damage to the cochlea, including noise exposure, ischemia, surgical trauma, and aminoglycoside, resulting in hearing recovery.

Perspective on an Innovative Curative Strategy for Peritoneal Metastasis Involving Peritonectomy, Hyperthermic Intraperitoneal Chemotherapy, and Adjuvant Chemotherapy Identified as Effective in the Histoculture Drug Response Assay (HDRA).

Background/aim: Peritoneal carcinomatosis is the end stage for patients with gastrointestinal cancer, with survival ranging between 2 and 9 months. Pancreatic acinar cell carcinoma (PACC) is rare and can result in peritoneal metastases. The efficacy of chemotherapy for patients with PACC is unknown, and a systemic treatment strategy has not been established.

Recombinant Methioninase and Cisplatinum Act Synergistically to Inhibit Lewis Lung Carcinoma Cells But Not Normal Fibroblasts.

Background/aim: Cisplatinum (CDDP) is used as first-line therapy in lung cancer. To further improve the therapeutic efficacy of CDDP, we focused on methionine addiction of cancer, known as the Hoffman effect. The present study aimed to determine the synergistic efficacy of the combination of CDDP and recombinant methioninase (rMETase) to target methionine addiction of lung cancer.

HT1080 Human Fibrosarcoma Cells Selected for Super-eribulin Resistance Become More Malignant and Are Arrested Synergistically by Methionine Restriction in Combination With Eribulin in Nude Mice.

Background/aim: Eribulin is a microtubule inhibitor used in the treatment of various malignancies, including soft-tissue sarcoma. However, the development of eribulin resistance is a recalcitrant clinical problem. The present study demonstrates that super eribulin-resistant HT1080 human fibrosarcoma cells become highly malignant but can be eradicated synergistically by the combination of eribulin and methionine restriction in nude mice.

Association of type 2 diabetes mellitus and cognitive function with the resumption of oral intake during stroke rehabilitation.

Background: Physical factors including motor and balance functions are associated with resumption of oral intake of the stroke patients receiving nasogastric tube feeding. However, association of non-physical factors remains unclear. The aims of this study are to explore non-physical factors associated with resumption of oral intake after stroke and to clarify how they affect the time course of the resumption.

Digital health interventions for non-older individuals at risk of frailty: A systematic review and meta-analysis.

Objective: Frailty is a significant health problem that results in adverse outcomes, reduced quality of life, and increased medical and nursing care costs. This study aimed to review the effect of digital health interventions on improving physical activity, physical function, and social function in non-older individuals at risk of frailty.

Methods: Randomized controlled trials of digital health interventions reporting frailty-related outcomes on physical activity, physical function, and social function in non-older individuals at risk of frailty were collected.

Reporting and Methodological Qualities of Systematic Reviews in Rehabilitation Journals After 2020: A Cross-Sectional Meta-Epidemiological Study.

Objectives: The aim of this study was to investigate the reporting and methodological qualities in systematic reviews (SRs) of rehabilitation journals following updating to the use of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 statement.

Methods: SRs with pairwise meta-analyses on the effects of health interventions were selected, which were published in rehabilitation journals in 2020, 2021, and 2022 using MEDLINE (PubMed). Exposure was defined as reporting use of the PRISMA 2020 statement.

Highly Synergistic Eradication of 143B Osteosarcoma Cells by the Combination of Recombinant Methioninase, Chloroquine, and Rapamycin Targeting Methionine Addiction, Autophagy, and mTOR, Respectively.

Background/aim: Drug-resistant osteosarcoma is a highly aggressive malignancy with limited therapeutic options. Recombinant methioninase (rMETase) targets the methionine addiction of cancer and acts synergistically with many cancer-chemotherapy agents. The present study investigated the synergistic efficacy of the combination of rMETase, chloroquine (CQ) which targets autophagy, and rapamycin (RAPA) which targets mTOR, on human 143B osteosarcoma cells .

Enhancing Reporting Quality Using the Preferred Reporting Items for Systematic Review and Meta-Analysis 2020 in Systematic Reviews of Emergency Medicine Journals: A Cross-Sectional Study.

Background: Systematic reviews (SRs) with thorough reporting and rigorous methodology lead to less biased outcomes. The Priority Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement was developed to enhance SRs and meta-analysis reporting. While it was updated to PRISMA 2020, the impact on emergency medicine remains unexplored.

HT1080 Fibrosarcoma With Acquired Trabectedin Resistance: Increased Malignancy But Sustained Sensitivity to Methionine Restriction.

Background/aim: Trabectedin is a DNA-binding agent that has shown moderate efficacy for soft-tissue sarcomas. We have previously shown that methionine restriction enhances trabectedin efficacy on both parental and trabectedin-resistant HT1080 (TR-HT1080) cells The aim of the present study was to determine whether fibrosarcoma cells that acquire trabectedin resistance become more malignant but maintain sensitivity to methionine restriction Materials and Methods: TR-HT1080 was established by culturing HT1080 cells in stepwise increasing concentrations of trabectedin. An wound-healing invasion assay was used to compare malignancy of HT1080 and TR-HT1080.

Dynamic Fluorescence Imaging of Orally-administered Tumor-targeting A1-R Expressing Green Fluorescent Protein Trafficking Through the Gastrointestinal System to Target Fibrosarcomas in Nude Mice.

Background/aim: A1-R (A1-R) expresses green fluorescent protein (GFP) and has the ability to selectively target and inhibit all major cancer types in murine models without persistently infecting healthy tissue. A1-R is being developed for tumor targeting by oral administration. The aim of the present study was to demonstrate real-time imaging of orally-administered A1-R in a fibrosarcoma nude-mouse model and to visualize its trafficking through the gastrointestinal system to the tumor and normal organs.

Targeted Elimination of Influenza Virus and Infected Cells with Near-Infrared Antiviral Photoimmunotherapy (NIR-AVPIT).

: Seasonal influenza causes significant morbidity and mortality each year. Since viruses can easily acquire drug-resistant mutations, it is necessary to develop new antiviral strategies with different targets. Near-infrared photoimmunotherapy (NIR-PIT) is a type of anti-cancer therapy that has recently attracted considerable attention, with favorable outcomes reported for several cancers.

Classifications and treatment management of fragility fracture of the pelvis: A scoping review.

Background: Fragility fractures of the pelvis (FFP) present a growing challenge in aging populations. However, standardized classifications and treatment guidelines remain scarce.

Objective: This scoping review examines the application of fracture classifications, treatment strategies, and outcome evaluations for FFP, identifying gaps in the literature, and suggesting directions for future research.

Recombinant Methioninase (rMETase) Synergistically Sensitizes Ivermectin-resistant MCF-7 Breast Cancer Cells 9.9 Fold to Low-dose Ivermectin.

Background/aim: Ivermectin is a widely-used anti-parasitic agent and has shown early promise as an anticancer agent. Recombinant methioninase (rMETase) is a methionine-depleting enzyme targeting the methionine addiction of cancer and has broad efficacy against all tested cancer types. However, the combination efficacy of ivermectin and rMETase on breast cancer cells remains unexplored.