Publications by authors named "Nikhil Kc"
Role of micronutrients in production and reproduction of farm animals under climate change scenario.
Trop Anim Health Prod
January 2025
Climate change poses significant challenges to livestock production worldwide. Wherein, it affects communities in developing nations primarily dependent on agriculture and animal husbandry. Its direct and indirect deleterious effects on agriculture and animal husbandry includes aberrant changes in weather patterns resulting in disturbed homeorhetic mechanism of livestock vis a vis indirectly affecting nutrient composition of feed and fodder.
View Article and Find Full Text PDFDeletion of both anaerobic regulator genes fnr and narL compromises the colonization of Salmonella Typhimurium in mice model.
World J Microbiol Biotechnol
November 2024
Salmonella Typhimurium (STM), a zoonotic pathogen, can adjust its metabolic pathway according to the variations in the partial pressure of atmospheric oxygen and nitrate via fumarate nitrate reductase regulator (Fnr) and NarL, the response regulator for nitrate reductase. Both Fnr and NarL have been individually reported to be the contributors of virulent phenotypes of STM. Hypoxia along with nitrate-rich environment are prevalent in macrophages and the Salmonella-induced inflammatory lumen of the host's large intestine activates both fnr and narL genes.
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates how altering the fnr gene, which regulates anaerobic conditions, impacts the pathogenic features of Salmonella Typhimurium (STM).
- - Mutants with a recoded fnr gene showed decreased competition with wild strains under nutrient-poor conditions, reduced motility, less biofilm formation, and decreased survival within macrophages.
- - The recoded fnr strains also demonstrated significantly lower colonization in mice and reduced fecal shedding, indicating that changes in this regulatory gene can weaken the pathogenicity of STM.
Aim: The aim of this study was to understand the role of anaerobic regulator FNR (Fumarate Nitrate Reduction) in Salmonella Typhimurium through proteomic approach.
Methods And Results: We did label free quantitative proteomic analysis of Salmonella Typhimurium PM45 wild type and the fnr null mutant cultured under anaerobic conditions. The data revealed 153 significantly differentially expressed proteins (DEPs) in the mutant out of 1798 total proteins identified.