Antibody-functionalized lipid nanocarriers for RNA-based cancer gene therapy: advances and challenges in targeted delivery.

Despite remarkable advances in cancer therapeutics, conventional treatments still face significant hurdles, including systemic toxicity, poor tumor specificity, multidrug resistance, and suboptimal intracellular delivery. Lipid-based nanocarriers (LBNCs) have emerged as versatile platforms for delivering therapeutic RNA molecules, offering biocompatibility and tunable properties that enhance drug stability and bioavailability. Functionalizing these nanocarriers with antibodies has unlocked new potential for achieving precise tumor targeting, leveraging the overexpression of specific receptors on cancer cells.

Mitogenomic characterization and phylogenetic placement of the Red pandora Pagellus bellottii from the Eastern Atlantic Ocean within the family Sparidae.

The systematics and conservation management of the Red pandora (Pagellus bellottii) remain challenging due to the overlapping morphology and distributions with closely related congeners in the Eastern Atlantic Ocean and Mediterranean Sea. These challenges are further exacerbated by population declines driven by intensive coastal fisheries and a paucity of molecular data. In this study, high-throughput sequencing was utilized to assemble the first complete mitogenome of P.

Mitogenomic Profiling of (Cypriniformes: Cyprinidae) and Its Phylogenetic Placement Within the Clade "Poropuntiinae".

The systematic status of the genus remains ambiguous due to a lack of comprehensive morphological and molecular evidence. Hence, this study represents the novel mitogenome of to characterize its architecture and clarify its phylogenetic placement within the clade "Poropuntiinae." The circular mitogenome was 16,571 base pairs in length and comprised 37 genes and a control region (CR).

Concurrent intravenous immunoglobulin and platelet transfusion for refractory alloimmune thrombocytopenia in patients undergoing allogeneic hematopoietic stem cell transplantation.

Background: Severe refractory alloimmune thrombocytopenia is a challenging and life-threatening complication in patients with hematologic disorders who are undergoing allogeneic hematopoietic stem cell transplantation. This study aimed to investigate the utility of continuous intravenous immunoglobulin and platelet transfusions as a therapeutic approach for alloimmune thrombocytopenia in patients undergoing allogeneic transplants.

Methods: A single-center retrospective analysis was conducted of ten adult allogeneic transplant patients hospitalized with transfusion-refractory alloimmune thrombocytopenia.

Tailoring of Prematurely Released Polyketide Intermediates in Piericidin Biosynthesis.

Piericidins make up a group of microbial-derived polyketide natural products with a wide variety of biological activities. Their biosynthetic gene clusters (BGCs) have been identified in several strains of . In , the piericidin BGC splits into two loci consisting of genes that encode six modular (type I) polyketide synthases and five post polyketide synthase (PKS) modification enzymes.

Multidimensional Determinants of Functional Status in Patients with Heart Failure.

Introduction: Functional status is a predictor of rehospitalization and mortality in patients with heart failure (HF). The purpose of this study was to test the variables in the Multidimensional Model of Functional Status (MMFS) as determinants of functional status.

Methods: Using structural equation modelling, we analysed data from 520 patients with HF to determine the best multivariate model of functional status.

Harnessing de-oiled seed-anchored-CuO nanoparticles for adsorptive removal of crystal violet dye with comprehensive mechanistic insights.

A novel solution is introduced by harnessing the power of de-oiled L. seeds (DO/S) and enhancing them with copper oxide-loaded nanoparticles (DO/S NPs) to effectively eradicate the toxic dye crystal violet (triarylmethane dye) from aqueous solutions. The presence of peaks at 653.

Role of an Auxiliary Donor and Ethynyl π-Spacer in a New Phenothiazine Derivative as a Dye for Dye-Sensitized Solar Cells.

A new donor-donor-π-acceptor 2-cyano-3-(7-(2-(4-methoxyphenyl)ethynyl)-10-ethyl-10H-phenothiazin-3-yl)acrylic acid (PEDCA) has been synthesized in good yield and was characterized using nuclear magnetic resonance (NMR) and Fourier transform infrared spectroscopy (FTIR) spectroscopies. The obtained phenothiazine (PTZ) derivative was explored in dye-sensitized and tandem dye-sensitized solar cells (TDSCs). For reference purposes, the devices based on commercial organometallic dye N719 and a similar PTZ-based dye but without auxiliary donor and ethynyl π-spacer, that is, 2-cyano-3-(10-ethyl-10H-phenothiazin-3-yl)acrylic acid (PEC) were used.

Machine learning driven dashboard for chronic myeloid leukemia prediction using protein sequences.

The prevalence of Leukaemia, a malignant blood cancer that originates from hematopoietic progenitor cells, is increasing in Southeast Asia, with a worrisome fatality rate of 54%. Predicting outcomes in the early stages is vital for improving the chances of patient recovery. The aim of this research is to enhance early-stage prediction systems in a substantial manner.

Impact of FLT3 inhibitors on the outcomes of FLT3-ITD mutated acute myeloid leukemia following allogeneic hematopoietic stem cell transplant: A systematic review and meta-analysis.

Background: Acute Myeloid Leukemia (AML) with FLT3-ITD mutations is associated with high post-transplant relapse rates. FLT3 inhibitor (FLT3i) maintenance therapy following allogeneic hematopoietic stem cell transplantation (allo-HCT) has emerged as a promising strategy to improve outcomes in this high-risk population.

Methods: We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) evaluating FLT3i maintenance therapy versus standard of care (SOC) after allo-HCT in patients with FLT3-ITD-mutated acute myeloid leukemia (AML).

Towards digital transformation: A Quality by Design approach to plant-based nanostructured lipid carriers formulation.

This study establishes a streamlined, scalable, and reproducible workflow for the sustainable and computationally guided development of nanostructured lipid carriers (NLCs) by integrating Quality by Design and molecular simulations with sustainable approaches in formulating plant-based NLCs loaded with curcumin (CUR) as a model natural molecule. Our approach aims to accelerate the screening, optimization, and development processes of nanoformulations while maintaining drug loading and stability and adhering to green chemistry principles. A combined-mixture process and a 3 Factorial/RSM experimental design were used.

Policies and programmes to improve preconception nutrition in South Asia.

The health and health behaviours of women before conception significantly influence maternal and child health outcomes. Despite growing evidence supporting preconception nutrition care, data on the implementation of related policies and programmes remains limited. This paper reviews public policies and programmes delivering preconception nutrition interventions in eight South Asian countries, targeting married pre-pregnant women aged 15-49 years and identifies the systems bottlenecks in programme implementation.

A New Mixed Biased Estimator for Ill-Conditioning Challenges in Linear Regression Model With Chemometrics Applications.

In linear regression models, the ordinary least squares (OLS) method is used to estimate the unknown regression coefficients. However, the OLS estimator may provide unreliable estimates in non-orthogonal models. This article introduces a novel mixed-biased estimator to address the challenges posed by the non-orthogonal model.

Attitudes and perspectives of oncologists about measurable residual disease testing in multiple myeloma: initiative for standardizing guidelines.

Measurable residual disease (MRD) testing is emerging as a crucial prognostic and treatment-guiding tool in multiple myeloma (MM). Despite its potential, routine clinical adoption remains inconsistent. This study aimed to explore oncologists' perspectives on MRD testing and identify barriers and facilitators to its clinical integration.

Glucuronoxylan hemicellulose-based manganese oxide nanoparticles for enhanced bactericidal, wound healing, and photocatalytic potential.

Hemicelluloses are promising candidates for synthesizing nanosystems for potential biomedical and photocatalytic applications. Glucuronoxylan (hemicellulose)-capped manganese oxide nanoparticles (GX-MnO NPs) were synthesized from quince ( M.) seed hydrogel.

Enhancing faculty teaching performance through constructive leadership with a mediating role of job satisfaction.

This study explored the impact of constructive leadership styles including transformational leadership (TLS), authentic leadership (ALS), and servant leadership (SLS) on faculty teaching performance (FTP), with job satisfaction (JS) acting as a critical mediator. Using a cross-sectional design and convenience sampling, data were collected from 346 faculty members across six universities in Lahore, Pakistan. Structural equation modeling (SEM) and regression analysis revealed that all three leadership styles significantly enhanced FTP, with transformational leadership showing the strongest influence.

Mitogenomic Characterization and Phylogenetic Insights of the Ornamental Sail-Fin Molly (Poecilia velifera) in Non-Native Indonesian Waters.

The ornamental fish Poecilia velifera (Sail-fin molly, Poeciliidae) has spread widely to various non-native ecosystems around the world, far from its native habitat in the Yucatan Peninsula, Mexico. Despite the availability of some partial mitochondrial and nuclear genetic information, the complete mitogenomic structure and its variation remain unknown for this species, which is essential for a comprehensive genetic characterization and detailed phylogenetic investigation. This study applied next-generation sequencing to generate the de novo mitogenome of morphologically identified P.

Improved Photodynamic Therapy of Hepatocellular Carcinoma via Surface-Modified Protein Nanoparticles.

Photodynamic therapy (PDT) has evolved as a reliable therapeutic modality for cancer. However, the broad application of the technique is still limited because of poor bioavailability and the non-selective distribution of photosensitizers within host tissues. Herein, zein, a natural corn protein, was functionalized with glycyrrhetinic acid (GA) and polyethylene glycol (Z-PEG-GA) as a targeting platform for liver cancer cells.

Quality of Experience (QoE) in Cloud Gaming: A Comparative Analysis of Deep Learning Techniques via Facial Emotions in a Virtual Reality Environment.

Cloud gaming has rapidly transformed the gaming industry, allowing users to play games on demand from anywhere without the need for powerful hardware. Cloud service providers are striving to enhance user Quality of Experience (QoE) using traditional assessment methods. However, these traditional methods often fail to capture the actual user QoE because some users are not serious about providing feedback regarding cloud services.

Semisynthesis and biological activities of derivatives of cyclocommunol from fruit peel.

(breadfruit) is an economically important species of the Moraceae family found throughout the tropics. While breadfruit pulp is widely used and processed into various food products, the peel waste is currently underutilised. Our chemical study of breadfruit peel has led to the isolation of the major constituent cyclocommunol ().

Comparative evaluation of management practices among large-scale broiler farms of Punjab, Pakistan.

Limited research is available on the management practices of commercial broiler chicken production in Pakistan. Notably, the rapid expansion of environmentally controlled broiler farms in Punjab in recent years remains underexplored, with scarce information on this growing enterprise. The current study compared the management practices at 100 commercial broiler farms in Punjab, Pakistan, among different regions.

Outcomes with Allogeneic Hematopoietic Stem Cell Transplantation in Therapy Related Myeloid Neoplasms: A Systematic Review and Meta-Analysis.

Therapy-related myeloid neoplasms (t-MN), which include acute myeloid leukemia (t-AML), myelodysplastic syndrome (t-MDS), and myelodysplastic/myeloproliferative neoplasms are secondary malignancies occurring as a late complication following chemotherapy or radiation therapy for an antecedent disorder. Allogeneic hematopoietic stem cell transplant (allo-HCT) is a potentially curative treatment option in t-MN patients. This systematic review and meta-analysis aimed to explore the outcomes of allo-HCT in t-MN.

Demystifying the role of MMP9 and TIMP-1 as markers of lung imaging and functional abnormality of COVID-19.

Introduction: This study aimed to analyze the levels of MMP-9 and TIMP-1 as biomarkers for identifying lung anatomical and functional abnormalities in coronavirus disease 2019 (COVID-19).

Methodology: Adult COVID-19 patients hospitalized between October and December 2021 were included in the study. MMP-9 and TIMP-1 levels were measured from the blood.

Genomic, Molecular Networking-Based Metabolomic, and Bioactivity Profiling of Actinobacteria from Undisturbed Caves in Pakistan.

Caves are a unique ecosystem that harbor diverse microorganisms, and provide a challenging environment to the dwelling microbial communities, which may boost gene expression and can lead to the production of inimitable bioactive natural products. In this study, we obtained 59 actinobacteria from four different caves located in Bahadurkhel, District Karak, Pakistan. On the basis of taxonomic characteristics, 30 isolates were selected and screened for secondary metabolites production and bioactivity profiling.