Comparative metabolomic analysis of an alternative biosynthetic pathway to pseudosugars in Actinosynnema mirum DSM 43827.

Through a combination of genome mining, gene knockouts, and comparative metabolomic studies, a new biosynthetic pathway to validoxylamine A has been discovered in DSM 43827. The study revealed the rich diversity of biosynthetic pathways to pseudosugar-containing natural products.

Mutasynthesis of fluorinated pactamycin analogues and their antimalarial activity.

A mutasynthetic strategy has been used to generate fluorinated TM-025 and TM-026, two biosynthetically engineered pactamycin analogues produced by Streptomyces pactum ATCC 27456. The fluorinated compounds maintain excellent activity and selectivity toward chloroquine-sensitive and multidrug-resistant strains of malarial parasites as the parent compounds. The results also provide insights into the biosynthesis of 3-aminobenzoic acid in S.