Evaluation Strategies for Tissue-engineered Tracheas: From Characterization to Assessment.

The trachea plays a critical role in maintaining airway patency, ventilation, and mucociliary clearance, supported by its unique anatomical and structural features. Tracheal defects resulting from congenital anomalies, malignancies, trauma, or prolonged intubation present significant clinical challenges. Traditional reconstruction methods, such as end-to-end anastomosis and patch grafts, are often limited by technical feasibility and suboptimal outcomes.

Artificial uterus with fluidics-driven system using material-switching digital light processing 3D bioprinting.

Despite various attempts to replicate complex organ structures using 3D bioprinting technologies, the fabrication of a tissue-engineered endometrium with integrated vasculature remains a significant challenge in the field. In this study, we developed three bioinks by combining glycidyl methacrylate-modified GelMA (GelMAGMA) hydrogel precursor with endometrial stem cells, stromal cells, and endothelial cells to create a vascularized endometrial construct. Utilizing a one-step material-switching DLP 3D bioprinter capable of multi-material printing, we successfully fabricated an engineered endometrial construct with a vascular channel extending through both the functional and basal layers.

A Novel Porous Coating Method of Commercially Pure Titanium Using Silk Fibroin and UV Light for Biomedical Implant Applications.

Surface coatings for titanium implants have been actively investigated using numerous materials to improve biocompatibility and osteointegration. This study developed a novel porous coating method for titanium implants; we coated commercially pure titanium (Cp-Ti) using the photocurable properties of methacrylated silk fibroin (SilMA). Surface morphologies and alloying chemistry after coating were investigated by scanning electron microscopy (SEM) and EDS, while the biocompatibility of the SilMA-coated Cp-Ti was evaluated by the CCK-8 and live/dead assays.

enhancement of CD8+ T cell activity using functionalized hydrogel encapsulating tonsil-derived lymphatic endothelial cells.

This study investigates a method for programming immune cells using a biomaterial-based system, providing an alternative to traditional cell manipulation techniques. It addresses the limitations of engineered adoptive T cell therapies, such as T cell exhaustion, by introducing a gelatin-hyaluronic acid (GH-GMA) hydrogel system. We characterized tonsil mesenchymal stem cells (TMSCs), lymphatic endothelial cells (T-LECs), stimulated T-CD8 T cells (STCs), and GH-GMA biomaterials.

Transcriptomic Changes in Human Tonsil-Derived Mesenchymal Stem Cells Across Culture Passages.

Background/objectives: Tonsil-derived mesenchymal stem cells (TMSCs) are in the limelight in regenerative medicine due to their high proliferation and differentiation potential. It is important to conduct studies to determine the optimal conditions for achieving the maximum yield while maintaining the optimal differentiation capacity of TMSCs.

Methods: This study explores the impact of serial subculture on TMSCs by analyzing gene expression at passages 2, 4, 6, and 8.

Highly water-soluble diacetyl chrysin ameliorates diabetes-associated renal fibrosis and retinal microvascular abnormality in db/db mice.

Background/objectives: Chronic or intermittent hyperglycemia is associated with the development of diabetic complications. Oxidative stress and inflammation can be altered by hyperglycemia in diverse tissues, including kidneys and eyes, and play a pivotal role in diabetic complications. Our previous studies showed that the water-insoluble 5,7-dihydroxyflvone chrysin effectively combats diabetic damages incurred in diabetic kidneys and retinas.

Aesculetin Accelerates Osteoblast Differentiation and Matrix-Vesicle-Mediated Mineralization.

The imbalance between bone resorption and bone formation in favor of resorption results in bone loss and deterioration of bone architecture. Osteoblast differentiation is a sequential event accompanying biogenesis of matrix vesicles and mineralization of collagen matrix with hydroxyapatite crystals. Considerable efforts have been made in developing naturally-occurring plant compounds, preventing bone pathologies, or enhancing bone regeneration.