Non-O1/O139 environmental Vibrio cholerae from Northern Cameroon reveals potential intra-/inter-continental transmissions.

Northern Cameroon in Central Africa has experienced recurring cholera outbreaks despite ongoing efforts to control the disease. While most cholera studies focus on O1 pandemic Vibrio cholerae strains, non-O1/O139 strains are increasingly recognized for their infection potential and dynamic relationships with O1 strains during outbreaks. Here we explore the genetic diversity and phylogenetic relationships of non-O1/O139 V.

O47 associated with a cholera-like diarrheal outbreak concurrent with seasonal cholera in Bangladesh.

The Ganges delta of the Bay of Bengal is a recognized hotspot for the emergence and spread of novel variants of . Despite being a diverse species, very little information is available concerning environmental and human-associated aspects of serogroups, other than the two major epidemic-related serogroups O1 and O139. This represents a crucial gap in understanding the spectrum of diversity, ecology, and epidemiology of the species influencing the dynamics of global cholera.

Capturing dynamic phage-pathogen coevolution by clinical surveillance.

Bacteria harness diverse defense systems that protect against phage predation, many of which are encoded on horizontally transmitted mobile genetic elements (MGEs). In turn, phages evolve counter-defenses, driving a dynamic arms race that remains underexplored in human disease contexts. For the diarrheal pathogen , a higher burden of its lytic phage, ICP1, in patient stool correlates with reduced disease severity.

The Stability of UV-Defluorination-Driven Crosslinked Carbon Nanotubes: A Raman Study.

Carbon nanotubes (CNTs) are often regarded as semi-rigid, all-carbon polymers. However, unlike conventional polymers that can form 3D networks such as hydrogels or elastomers through crosslinking in solution, CNTs have long been considered non-crosslinkable under mild conditions. This perception changed with our recent discovery of UV-defluorination-driven direct crosslinking of CNTs in solution.

Vibrio cholerae O1 associated with recent endemic cholera shows temporal changes in serotype, genotype, and drug-resistance patterns in Bangladesh.

Background: Despite the advancement in our understanding of cholera and its etiological agent, Vibrio cholerae, the prevention and treatment of the disease are often hindered due to rapid changes in drug response pattern, serotype, and the major genomic islands namely, the CTX-prophage, and related genetic characteristics. In the present study, V. cholerae (n = 172) associated with endemic cholera in Dhaka during the years 2015-2021 were analyzed for major phenotypic and genetic characteristics, including drug resistance patterns.

Genomic attributes of Vibrio cholerae O1 responsible for 2022 massive cholera outbreak in Bangladesh.

In 2022, one of its worst cholera outbreaks began in Bangladesh and the icddr,b Dhaka hospital treated more than 1300 patients and ca. 42,000 diarrheal cases from March-1 to April-10, 2022. Here, we present genomic attributes of V.

sp. nov., a novel member of the Cholerae clade.

A number of bacteria with close resemblance to have been isolated over the years by the Centres for Disease Control and Prevention (CDC), which could not be assigned a proper taxonomic designation on the basis of the results from preliminary identification methods. Nine such isolates have been found to share 16S rRNA gene identity exceeding 99 % with yet DNA-DNA hybridization (60.4-62.

Vibrio cholerae O1 El Tor strains linked to global cholera show region-specific patterns by pulsed-field gel electrophoresis.

Vibrio cholerae O1 El Tor, causative agent of the ongoing seventh cholera pandemic, is native to the aquatic environment of the Ganges Delta, Bay of Bengal (GDBB). Recent studies traced pandemic strains to the GDBB and proposed global spread of cholera had occurred via intercontinental transmission. In the research presented here, NotI-digested genomic DNA extracted from V.

Dynamic Subspecies Population Structure of Vibrio cholerae in Dhaka, Bangladesh.

Cholera has been endemic to the Ganges Delta for centuries. Although the causative agent, Vibrio cholerae, is autochthonous to coastal and brackish water, cholera occurs continually in Dhaka, the inland capital city of Bangladesh which is surrounded by fresh water. Despite the persistence of this problem, little is known about the environmental abundance and distribution of lineages of V.

Computational screening of 645 antiviral peptides against the receptor-binding domain of the spike protein in SARS-CoV-2.

The receptor-binding domain (RBD) of SARS-CoV-2 spike (S) protein plays a vital role in binding and internalization through the alpha-helix (AH) of human angiotensin-converting enzyme 2 (hACE2). Thus, it is a potential target for designing and developing antiviral agents. Inhibition of RBD activity of the S protein may be achieved by blocking RBD interaction with hACE2.

Population Analysis of Vibrio cholerae in Aquatic Reservoirs Reveals a Novel Sister Species ( sp. nov.) with a History of Association with Humans.

Most efforts to understand the biology of Vibrio cholerae have focused on a single group, the pandemic-generating lineage harboring the strains responsible for all known cholera pandemics. Consequently, little is known about the diversity of this species in its native aquatic environment. To understand the differences in the V.

Simultaneous Quantification of and with Its O1 Serogroup and Toxigenic Subpopulations in Environmental Reservoirs.

is a recently described aquatic bacterium and opportunistic pathogen, closely related to and often coexisting with To study the relative abundance and population dynamics of both species in aquatic environments of cholera-endemic and cholera-free regions, we developed a multiplex qPCR assay allowing simultaneous quantification of total and (including toxigenic and O1 serogroup) cells. The presence of was restricted to samples from regions that are not endemic for cholera, where it was found at 20% of the abundance of . In this environment, non-toxigenic O1 serogroup represents almost one-fifth of the total population.

A Vibrio cholerae Core Genome Multilocus Sequence Typing Scheme To Facilitate the Epidemiological Study of Cholera.

Core genome multilocus sequence typing (cgMLST) has gained popularity in recent years in epidemiological research and subspecies-level classification. cgMLST retains the intuitive nature of traditional MLST but offers much greater resolution by utilizing significantly larger portions of the genome. Here, we introduce a cgMLST scheme for , a bacterium abundant in marine and freshwater environments and the etiologic agent of cholera.

Draft Genome Sequences of Eight sp. Clinical Isolates from across the United States That Form a Basal Sister Clade to Vibrio cholerae.

We sequenced the genomes of eight isolates from various regions of the United States. These isolates form a monophyletic cluster clearly related to but distinct from Vibrio cholerae. Phylogenetic and genomic analyses suggest that they represent a basal lineage highly divergent from Vibrio cholerae or a novel species.

Draft Genome Sequences of Nine sp. Isolates from across the United States Closely Related to Vibrio cholerae.

We are reporting whole-genome sequences of nine Vibrio sp. isolates closely related to the waterborne human pathogen Vibrio cholerae. These isolates were recovered from sources, including human samples, from different regions of the United States.