Mast cells modulate macrophage biology through release of prestored CSF1.

Background: Mast cells (MCs) are tissue-resident immune cells present in connective tissues throughout the body. They exert diverse functions in immunity by rapidly releasing a plethora of preformed mediators, including proteoglycans, cytokines, and proteases, which are stored in cytoplasmic granules.

Objective: We sought to systematically and globally identify MC-released protein mediators and elucidate their functions.

Sharing Pollinators and Viruses: Virus Diversity of Pollen in a Co-Flowering Community.

Co-flowering plant species frequently share pollinators, flower-inhabiting bacteria, and fungi, but whether pollen-associated viruses are shared is unknown. Given that pollen-associated viruses are sexually transmitted diseases, their diversity is expected to increase with pollinator sharing. We conducted a metagenomic study to identify pollen-associated viruses from 18 co-flowering plant species to determine whether 1) life history, floral traits, or pollination generalism were associated with viral richness, and 2) plants shared pollen-associated viruses.

Risk Factors of Long-Term Opioid Use After Elective Cervical and Lumbar Spine Surgery: A Michigan Spine Surgery Improvement Collaborative Study.

Background And Objectives: Given the current opioid epidemic and its impact on public health, long-term opioid use after elective spine surgery is a significant concern. Identifying risk factors of long-term opioid use after spine surgery is crucial for developing effective interventions to reduce opioid dependence in this patient population. The objective of this study was to identify risk factors associated with long-term opioid use after elective lumbar and cervical spine surgeries.

Intestinal helminths of bats in the States of Nayarit and Veracruz, Mexico, with redescription of Bidigiticauda vivipara Chitwood.

Although surveys on bat parasites in the Americas have been conducted, information on helminths remains limited. In Mexican states such as Nayarit and Veracruz only 1 and 8 helminth species have been reported in bats, respectively. In this study, we provide new helminthological records for bats using morphological techniques.

An extremely acidic environment microalga from Tinto River as a novel source of anti-inflammatory activity.

Coccomyxa onubensis (C. onubensis) is an acidotolerant microalga isolated from the extremely acidic Tinto River (Huelva), which contains high levels of metals in solution, mainly Fe and Cu. This makes C.

Equity Using Interventions for Pain and Depression (EQUIPD): A pilot randomized trial.

Despite increased calls for improved health equity, Black patients continue to experience worse pain and associated outcomes. Black patients are also offered fewer pain treatment options than White patients and report poorer quality communication with clinicians, including lower participation in shared decision-making. Comorbid depressive symptoms can impede effective pain management and participation in decision-making.

Immediate early splicing controls translation in activated T-cells and is mediated by hnRNPC2 phosphorylation.

The fast and transient induction of immediate early genes orchestrates the cellular response to various stimuli. These stimuli trigger phosphorylation cascades that promote immediate early gene transcription independent of de novo protein synthesis. Here we show that the same phosphorylation cascades also target the splicing machinery, inducing an analogous splicing switch that we call immediate early splicing (IES).

Aging platelets shift their hemostatic properties to inflammatory functions.

Platelets are crucial players in hemostasis and thrombosis but also contribute to immune regulation and host defense, using different receptors, signaling pathways, and effector functions, respectively. Whether distinct subsets of platelets specialize in these diverse tasks is insufficiently understood. Here, we used a pulse-labeling method in Mus musculus models for tracking in vivo platelet aging and its functional implications.

Hippocrepis fuelleborni (Digenea: Notocotylidae) from Argentina, morphological, molecular and phylogenetic studies.

Notocotylidae is a family with a cosmopolitan distribution, with 13 genera parasitizing birds and mammals. Four of these genera were registered in the Americas, and two parasitizing rodents in Argentina. In this paper, specimens of the genus Hippocrepis parasitizing Holochilus brasiliensis from Argentina are studied on the basis of morphological and molecular characteristics.

BDNF-TrkB signaling orchestrates the buildup process of local sleep.

Sleep debt accumulates during wakefulness, leading to increased slow wave activity (SWA) during sleep, an encephalographic marker for sleep need. The use-dependent demands of prior wakefulness increase sleep SWA locally. However, the circuitry and molecular identity of this "local sleep" remain unclear.

Sinusoidal Obstruction Syndrome/Veno-Occlusive Disease after Unmanipulated Haploidentical Hematopoietic Stem Cell Transplantation with Post-Transplantation Cyclophosphamide: A Study on Behalf of the Spanish Hematopoietic Stem Cell Transplantation and Cellular Therapy Group (GETH).

Sinusoidal obstruction syndrome/veno-occlusive disease (SOS/VOD) is a life-threatening complication after both autologous and allogeneic hematopoietic stem cell transplantation (HSCT). However, its characterization after haploidentical HSCT (haplo-HSCT) with post-transplantation cyclophosphamide (PT-Cy) is scarce. This study aimed to describe characteristics and outcomes of patients with SOS/VOD after haplo-HSCT with PT-Cy.

Disease flares with baricitinib dose reductions and development of flare criteria in patients with CANDLE/PRAAS.

Objectives: Patients with chronic atypical neutrophilic dermatosis with lipodystrophy and elevated temperature/proteasome-associated autoinflammatory syndrome (CANDLE/PRAAS) respond to the janus kinase inhibitor 1/2 inhibition with baricitinib at exposures higher than in rheumatoid arthritis. Baricitinib dose reductions to minimise exposure triggered disease flares which we used to develop 'flare criteria'.

Methods: Of 10 patients with CANDLE/PRAAS treated with baricitinib in an open-label expanded-access programme, baricitinib doses were reduced 14 times in 9 patients between April 2014 and December 2019.

Zoonotic nematode in the city of La Plata, Argentina: Report of a case of Calodium hepaticum in Rattus rattus.

This study describes a case of Calodium hepaticum (Trichinellida: Capillariidae) infection in an adult rat (Rattus rattus) from the periurban area of the city of La Plata in the province of Buenos Aires, Argentina. The rat was found with neurological signs (ataxia, lethargy, and episodes of unresponsiveness) in the food storage of a goat production facility. The liver was observed with hepatomegaly and diffuse and irregular yellowish-white spots appearing in striae or small nodules on the external surface and inside the liver.

High-sensitivity whole-genome recovery of single viral species in environmental samples.

Characterizing unknown viruses is essential for understanding viral ecology and preparing against viral outbreaks. Recovering complete genome sequences from environmental samples remains computationally challenging using metagenomics, especially for low-abundance species with uneven coverage. This work presents a method for reliably recovering complete viral genomes from complex environmental samples.

Ultrasound-Based Recovery of Anti-Inflammatory and Antimicrobial Extracts of the Acidophilic Microalga .

In the present study, the recovery of valuable molecules of proven anti-inflammatory and antimicrobial activity of the acidophilic microalga () were evaluated using green technologies based on ultrasound-assisted extraction (UAE). Using a factorial design (3 × 2) based on response surface methodology and Pareto charts, two types of ultrasonic equipment (bath and probe) were evaluated to recover valuable compounds, including the major terpenoid of , lutein, and the antimicrobial activity of the microalgal extracts obtained under optimal ultrasound conditions (desirability function) was evaluated versus conventional extraction. Significant differences in lutein recovery were observed between ultrasonic bath and ultrasonic probe and conventional extraction.

Malnutrition in COVID-19 survivors: prevalence and risk factors.

Background: Nutritional status is a critical factor throughout COVID-19 disease course. Malnutrition is associated with poor outcomes in hospitalized COVID-19 patients.

Aim: To assess the prevalence of malnutrition and identify its associated factors in COVID-19 survivors.

Exploring Social Determinants of Health in Healthy Aging Among Older Adults: A Qualitative Study.

Background: The U.S. population is aging and diversifying.

Association of the Stroke Ready Community-Based Participatory Research Intervention With Incidence of Acute Stroke Thrombolysis in Flint, Michigan.

Importance: Acute stroke treatment rates in the US lag behind those in other high-income nations.

Objective: To assess whether a hospital emergency department (ED) and community intervention was associated with an increased proportion of patients with stroke receiving thrombolysis.

Design, Setting, And Participants: This nonrandomized controlled trial of the Stroke Ready intervention took place in Flint, Michigan, from October 2017 to March 2020.

Alternative polyadenylation factor CPSF6 regulates temperature compensation of the mammalian circadian clock.

A defining property of circadian clocks is temperature compensation, characterized by the resilience of their near 24-hour free-running periods against changes in environmental temperature within the physiological range. While temperature compensation is evolutionary conserved across different taxa of life and has been studied within many model organisms, its molecular underpinnings remain elusive. Posttranscriptional regulations such as temperature-sensitive alternative splicing or phosphorylation have been described as underlying reactions.