Fabrication and characterization of novel β-sitosterol-loaded O/W Pickering emulsions stabilized by edible insects protein/chitosan complex coacervates: Retention and stability evaluation.

This study investigates the application of edible insect protein-chitosan (IP/CS) complex coacervates, derived from T. molitor, A. domesticus, and L.

Stabilization of double emulsions with anthocyanins using a plant protein-inositol hexaphosphate complex.

Background: Double emulsions (water-in-oil-in-water - W/O/W) offer a promising strategy for encapsulating sensitive bioactive compounds like anthocyanins. Their performance depends on the choice of stabilizing agents and the structural integrity of the interfacial layer, particularly under processing conditions such as spray drying. This study combined plant-based proteins (pea and rice) with inositol hexaphosphate (IP6) to investigate their coacervation behavior and effectiveness in stabilizing anthocyanin-loaded double emulsions.

Green sample preparation for anthocyanin extraction from purple corn: analytical evaluation of pressurized liquid and ultrasound-assisted extraction using sustainable solvents.

This study presents an analytical comparison of two extraction techniques-pressurized liquid extraction (PLE) and ultrasound-assisted extraction (UAE)-for the recovery of anthocyanins from purple corn using sustainable solvents (o-phosphoric acid, ethanol, and water). Twelve anthocyanins were identified using high-performance liquid chromatography with ultraviolet detection and tandem mass spectrometry, confirming method specificity and selectivity. Both extraction methods were optimized using multivariate experimental designs and validated following U.

Follow-up of the re-evaluation of silver (E 174) as a food additive (EFSA-Q-2023-00169).

Silver (E 174) is a food colour that was re-evaluated by the EFSA ANS Panel (2016). The ANS Panel concluded that the information available then, was insufficient to assess the safety of silver as food additive. The major issues included limited characterisation of silver E 174 (e.

Assessment of Lipid Peroxidation Products in Adult Formulas: GC-MS Determination of Carbonyl and Volatile Compounds Under Different Storage Conditions.

The occurrence of carbonyl compounds and volatile organic compounds (VOCs) in adult formulas is a critical issue in product safety and quality. This research manuscript reports the determination of targeted and untargeted carbonyl compounds and VOCs in adult formulas stored at different temperatures (room temperature, 4 °C, and 60 °C) over one month. Gas chromatography-mass spectrometry was utilized for the sample analysis.

Edible insect protein concentrates: Optimized salt-assisted extraction methods evaluation.

This study explores the extraction of proteins from edible insects such as Tenebrio molitor, Acheta domesticus, and Locusta migratoria using alkaline extraction and acid precipitation (AEAP) as a control method and evaluates the influence of salting-in (NaCl-assisted), salting-out ((NH₄)₂SO₄), and combined salting-in/out techniques on the resulting protein quality and functional properties. We hypothesized that salt-assisted methods would enhance protein extraction efficiency and functionality compared to AEAP. Molecular size distribution analysis confirmed that the salting-out method preferentially aggregated smaller proteins (<30 kDa).

Advancing protein hydrolysis and phytosterol encapsulation: Emerging trends and innovations in protein-based microencapsulation techniques - A comprehensive review.

Phytosterols represent a diverse and complex category of lipophilic bioactive compounds, exhibiting excellent pro-healthy properties. However, their consumption in daily diets is insufficient, and their application in food production is hindered by challenges such as low water solubility, high reactivity, and rapid degradation. The adoption of different protein or their structural modification as hydrolysates as wall material into microencapsulation techniques can be associated with improved solubility, enhanced bioaccessibility, increased bioavailability, and an extension of shelf life.

Polysaccharide-potato protein coacervates for enhanced anthocyanin bioavailability and stability.

Anthocyanins (ACNs) possess strong antioxidants, anti-cancer, anti-obesity, anti-diabetic, and anti-inflammatory properties but are limited use by their susceptibility to environmental factors. This study aims to overcome these limitations by developing and assessing a novel coacervate system, consisting of potato protein isolate (PPI) combined with various polysaccharides, to stabilize and encapsulate anthocyanins from black carrot concentrate The polysaccharides included in this system include inulin, gum Arabic, guar gum, pectin, and soluble fiber. The coacervate system's effectiveness in maintaining stability and increasing the bioavailability of anthocyanins was evaluated compared to conventional soybean protein-based systems.

Rapid and efficient high-performance liquid chromatography-ultraviolet determination of total amino acids in protein isolates by ultrasound-assisted acid hydrolysis.

This study presents the optimization and validation of an ultrasound-assisted acid method for the HPLC-UV determination of amino acids in plant-based proteins. The research focuses on enhancing hydrolysis efficiency and reducing environmental impact. Ultrasound treatment significantly accelerated hydrolysis by creating cavitation, which increases local pressure and temperature, leading to faster reaction rates.

Advances in Chromatographic Analysis of Phenolic Phytochemicals in Foods: Bridging Gaps and Exploring New Horizons.

Chromatographic analysis of phenolic phytochemicals in foods has significantly advanced over the past decade (2014-2024), meeting increasing demands for precision and efficiency. This review covers both conventional and advanced chromatographic techniques used for detecting phenolic phytochemicals in foods. Conventional methods like High-Performance Liquid Chromatography, Ultra High-Performance Liquid Chromatography, Thin-Layer Chromatography, and Gas Chromatography are discussed, along with their benefits and limitations.

Essential Oil Nanoemulsions-A New Strategy to Extend the Shelf Life of Smoothies.

Over the years, consumer awareness of proper, healthy eating has increased significantly, but the consumption of fruits and vegetables remains too low. Smoothie drinks offer a convenient way to supplement daily diets with servings of fruits and vegetables. These ready-to-eat beverages retain the nutritional benefits of the raw ingredients from which they are made.

Microencapsulation of green tea polyphenols: Utilizing oat oil and starch-based double emulsions for improved delivery.

The stability and bioavailability of green tea polyphenols, crucial for their health benefits, are compromised by environmental sensitivity, limiting their use in functional foods and supplements. This study introduces a novel water-in-oil-in-water double emulsion technique with microwave-assisted extraction, significantly enhancing the stability and bioavailability of these compounds. The primary objective of this study was to assess the effectiveness of several encapsulating agents, such as gum Arabic as control and native and modified starches, in improving encapsulated substances' stability and release control.

Microencapsulation of Essential Oils Using Faba Bean Protein and Chia Seed Polysaccharides via Complex Coacervation Method.

The aim of this study was to develop microcapsules containing juniper or black pepper essential oils, using a combination of faba bean protein and chia seed polysaccharides (in ratios of 1:1, 1:2, 2:1). By synergizing these two polymers, our goal was to enhance the efficiency of essential oil microencapsulation, opening up various applications in the food industry. Additionally, we aimed to investigate the influence of different polymer mixing ratios on the properties of the resulting microcapsules and the course of the complex coacervation process.

Simultaneous determination of six catechins and caffeine in tea and wine using salting-out assisted liquid-liquid extraction and high-performance liquid chromatography with ultraviolet detection.

Catechins, renowned for their antioxidant properties and health benefits, are commonly present in beverages, particularly tea and wine. An efficient and cost-effective salting-out assisted liquid-liquid extraction (SALLE) method has been developed and validated for the simultaneous determination of six catechins and caffeine in tea and wine samples using high-performance liquid chromatography-ultraviolet (HPLC-UV). This method demonstrates outstanding performance: linearity (1-120 µg/mL, r > 0.

A Review of Quantitative and Topical Analysis of Anthocyanins in Food.

Anthocyanins, a subclass of flavonoids known for their vibrant colors and health-promoting properties, are pivotal in the nutritional science and food industry. This review article delves into the analytical methodologies for anthocyanin detection and quantification in food matrices, comparing quantitative and topical techniques. Quantitative methods, including High-performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS), offer precise quantification and profiling of individual anthocyanins but require sample destruction, limiting their use in continuous quality control.

Deep eutectic solvents for the extraction of polyphenols from food plants.

Deep Eutectic Solvents (DESs) offer a promising, sustainable alternative for extracting polyphenols from food plants, known for their health benefits. Traditional extraction methods are often costly and involve toxic solvents. This review discusses the basic concepts, preparation techniques, and factors influencing the effective and safe use of DESs in polyphenol extraction.

Microencapsulation of Juniper and Black Pepper Essential Oil Using the Coacervation Method and Its Properties after Freeze-Drying.

Essential oils are mixtures of chemical compounds that are very susceptible to the effects of the external environment. Hence, more attention has been drawn to their preservation methods. The aim of the study was to test the possibility of using the classical model of complex coacervation for the microencapsulation of essential oils.

Novel Protein Sources for Applications in Meat-Alternative Products-Insight and Challenges.

Many people are increasingly interested in a vegetarian or vegan diet. Looking at the research and the available options in the market, there are two generations of products based on typical proteins, such as soy or gluten, and newer generation proteins, such as peas or faba beans, or even proteins based on previously used feed proteins. In the review, we present the characteristics of several proteins that can be consumed as alternatives to first-generation proteins used in vegan foods.

Evaluation of Modified Atmosphere Packaging in Combination with Active Packaging to Increase Shelf Life of High-in Beta-Glucan Gluten Free Cake.

Modified atmosphere packaging and active packaging were combined to prolong the shelf life and quality of the clean label, gluten-free (GF), yeast-leavened cakes enriched in oat fiber preparation. Star anise, cinnamon bark, and clove essential oils were used as emitters of active substances. The following concentrations of gases were chosen: 0% CO/100%/N (MAP), 60% CO/40% N (MAP), and approx.

Green Extraction of Carotenoids from Fruit and Vegetable Byproducts: A Review.

Carotenoids are characterized by a wide range of health-promoting properties. For example, they support the immune system and wound healing process and protect against UV radiation's harmful effects. Therefore, they are used in the food industry and cosmetics, animal feed, and pharmaceuticals.

Development of Gluten-Free Muffins with β-Glucan and Pomegranate Powder Using Response Surface Methodology.

More consumers are being diagnosed with celiac disease or diseases in which wheat products should be avoided. For this reason, it is important to increase the range of gluten-free products available. In this study, it was decided to optimize the technology for the creation of a muffin with β-glucan (BG) and pomegranate (PG), while establishing water share (WT), using the response surface methodology.

Plant-Based (Hemp, Pea and Rice) Protein-Maltodextrin Combinations as Wall Material for Spray-Drying Microencapsulation of Hempseed () Oil.

Conscious consumers have created a need for constant development of technologies and food ingredients. This study aimed to examine the properties of emulsions and spray-dried microcapsules prepared from hempseed oil by employing a combination of maltodextrin with hemp, pea, and rice protein as carrier materials. Oil content in the microcapsules was varied at two levels: 10 and 20%.

The impact of different levels of oat β-glucan and water on gluten-free cake rheology and physicochemical characterisation.

The demand for new gluten-free (GF) products is still very crucial issue in food industry. There is also a need for bioactive compounds and natural alternatives for food additives. For now, not only providing structure without gluten is major challenge, but also high sensory acceptance and nutritional value are on the top.

The Effect of Natural Antioxidants on Quality and Shelf Life of Beef and Beef Products.

Oxidation processes are the major cause of deterioration of meat quality and shelf life of meat products, leading to negative changes in nutritive value and in sensory and physicochemical properties of meat. Until now, the synthetic antioxidants like butylated hydroxyl anisole have been commonly used to prevent oxidation, which however may cause potential human health risks and increase toxicity of the product. However, natural antioxidants can be the alternative solution for this problem since plants and plant materials are rich in bioactive compounds (as natural antioxidants) with potential health beneficial effects.

Microencapsulation of Oil Using Selected Soluble Fractions of Dietary Fiber as the Wall Material.

The aim of the study was to prove the usefulness of microencapsulation of oil regarding its vulnerability to oxidation caused by oxygen, temperature, and other factors. Pectin, inulin, gum arabic, and β-glucan, each of them mixed with maltodextrin, were used as wall materials and their appropriability to reduce oxidation of the core material was examined. Microcapsules were prepared by spray drying, which is the most commonly used and very effective method.