Publications by authors named "Mahmoud Sitohy"

Following publication, concerns were brought to the attention of the Editorial Office regarding image inconsistencies within this study [...

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Introduction: This study investigated the tryptic hydrolysis of β-lactoglobulin (BLG) for 30, 60, 90, and 120 min at 1/200 E/S (enzyme/substrate ratio, w/w) to prepare potentially anticarcinogenic peptides.

Methods: The properties of hydrolysates were characterized, including degree of hydrolysis, free amino acids, SDS-PAGE, FTIR, and antioxidant activity employing DPPH-assay, β-carotene/linoleic acid, and FRAP assay.

Results: BLG tryptic hydrolysate produced after 60 min hydrolysis recorded the highest antioxidant activity, and LCMS analysis revealed 162 peptides of molecular masses ranging from 800 to 5671Da, most of them are of hydrophobic nature.

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  • - The study examined the effectiveness of Cowpea-legumin (CPL) in inhibiting antibiotic-resistant foodborne pathogens (FBP) and its impact on the quality and shelf-life of Longissimus thoracis et lumborum (LTL) steaks during 15 days of chilling at 4°C.
  • - Antibiotic-resistant Salmonella enterica and E. coli showed MICs of 25 and 3.125 mg/mL with CPL, while antibiotic-susceptible bacteria were effectively suppressed at 0.1 mg/mL.
  • - CPL not only enhanced the quality and color of the LTL steaks but also exhibited significant antioxidant and antibacterial properties, suggesting its potential as an effective preservative in combating antibiotic resistance
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  • The study highlights the urgent need for new antimicrobial and anticancer therapies due to the prevalence of life-threatening diseases and ineffective treatments.
  • Bee venom was collected and melittin extracted, then further broken down into bioactive peptides through hydrolysis and analyzed for their medicinal properties.
  • Results showed that the melittin fractions had strong antibacterial and anticancer effects on specific cancer cells while sparing normal cells, suggesting they may serve as effective new therapies for combating infections and cancer.
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  • * The study extracted anthocyanin-rich extracts from pomegranate peel, chili pepper fruit, and flowers, analyzing their biochemical composition and effectiveness through various assays.
  • * Results showed that pomegranate peel had the highest total phenolic content and exhibited significant antioxidant and antibacterial activities, while all extracts demonstrated the potential to inhibit cancer cell proliferation effectively.
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As antibiotics cannot inhibit multidrug-resistant bacteria (MDR), continuous research is mandatory to find other antibacterials from natural resources. Native legume proteins and their modified forms exhibited broad spectra of high antimicrobial activities. Sixteen bacterial isolates were mapped for antibiotic resistance, showing resistance in the range of (58-92%) and (42-92%) in the case of the Gram-negative and Gram-positive bacteria, respectively.

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The antibacterial and anti-biofilm activities of the 11S globulins isolated from lupin seeds (), and its methylated derivative (M11S), were investigated against seven pathogenic gram-positive and gram-negative bacteria. The MIC of 11S ranged from 0.1 to 4.

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The yellow polyphenolic pigment known as curcumin, originating from the rhizome of the turmeric plant L., has been utilized for ages in ancient medicine, as well as in cooking and food coloring. Recently, the biological activities of turmeric and curcumin have been thoroughly investigated.

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Heavy metal accumulation and pathogenic bacteria cause adverse effects on aquaculture. The active surface of selenium (Se) nanoparticles can mitigate these effects. The present study used Se-resistant AS12 to fabricate biological Se nanoparticles (Bio-SeNPs).

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The main target of this work is to discover new protein fractions from natural resources with high antibacterial action. The 7S and 11S globulin fractions, as well as the basic subunit (BS), were isolated from lupine seeds (), chemically characterized, and screened for antibacterial activity against seven pathogenic bacteria. SDS-PAGE revealed molecular weights ranging from 55 to 75 kDa for 7S globulin, 20-37 kD for 11S globulin, and 20 kD for the BS.

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Background: The world is facing an extraordinarily unprecedented threat from the COVID-19 pandemic triggered by the SARS-CoV-2 virus. Global life has turned upside down, and that several countries closed their borders, simultaneously with the blockage of life cycle as a result of the shutdown of the majority of workplaces except the food stores and some few industries.

Main Body: In this review, we are casting light on the nature of COVID-19 infection and spread, the persistence of SARS-CoV-2 virus in food products, and revealing the threats arising from the transmission of COVID-19 in food environment between stakeholders and even customers.

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Introduction: Biofungicides with low toxicity and high efficiency are a global priority for sustainable agricultural development. Phytohormone salicylic acid (SA) is an ancient medicine against various diseases in humans and activates the immune system in plants, but little is known of its function as a biofungicide.

Objectives: Here, Fusarium oxysporum, the causal agent of devastating Fusarium wilt and immunodepressed patients, was used as a model system to explore whether SA can enter the pathogen cells and suppress key targets of the pathogen.

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In recent years, significant progress has been achieved in genome editing applications using new programmable DNA nucleases such as zinc finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs) and the clustered regularly interspaced short palindromic repeats/Cas9 system (CRISPR/Cas9). These genome editing tools are capable of nicking DNA precisely by targeting specific sequences, and enable the addition, removal or substitution of nucleotides via double-stranded breakage at specific genomic loci. CRISPR/Cas system, one of the most recent genome editing tools, affords the ability to efficiently generate multiple genomic nicks in single experiment.

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Sixty-six (66) bacterial isolates were withdrawn from separate clinical samples of hospitalized patients with various clinical infections. Conventional bacteriological tests identified the species of all isolates, and standard microbiological techniques differentiated them into CoPS or CoNS. Their biofilm development was followed by an analysis via the MTP (microtiter tissue culture plates) technique, and we then investigated the presence/absence of and , which were qualified in the top-30 potent biofilm-forming isolates.

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Crustacean waste is one of the most severe issues, posing significant environmental and health risks. This study aims to improve managing marine waste by isolating chitosan from  by devising a new methodology, incorporating technical steps, e.g.

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  • The COVID-19 pandemic originated in Wuhan at the end of 2019, prompting urgent research into the virus's origins and the development of effective vaccines.
  • This study compares the genomes of SARS-CoV-2 (the virus causing COVID-19), SARS-CoV (2002), and MERS (2012), noting genetic differences that influence viral behavior and vaccine development.
  • The analysis reveals the role of specific genetic changes in the spike protein, revealing how COVID-19 variants emerged, and discusses various vaccine technologies that have been developed to combat the disease.
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The use of nanomaterials alone or in composites with proteins is a promising alternative to inhibit pathogenic bacteria. In this regard, this study used seed proteins from both fenugreek ( L.) (FNP) and mung bean () (MNP), with silver nanoparticles (Ag-NPs) and nanocomposites of either Ag-NPs plus FNP (Ag-FNP) or Ag-NPs plus MNP (Ag-MNP) as inhibitory agents against pathogenic bacteria.

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The present study examines the impacts of supplementing yogurt with 1% whey protein concentrate (WPC), Ca-caseinate (Ca-CN) and Spirulina platensis on the physiological performance of V-line rabbits receiving diets containing yogurt (at a dose of 5 g/kg body weight/day) and the different meat quality aspects. The results show that fat content was highest ( < 0.05) in yogurt fortified with Spirulina powder, but protein (%) was highest in yogurt enriched with WPC.

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A new preservation approach is presented in this article to prolong the lifetime of raw chicken meat and enhance its quality at 4 °C via coating with highly soluble kidney bean protein hydrolysate. The hydrolysates of the black, red, and white kidney protein (BKH, RKH, and WKH) were obtained after 30 min enzymatic hydrolysis with Alcalase (E/S ratio of 1:100, hydrolysis degree 25-29%). The different phaseolin subunits (8S) appeared in SDS-PAGE in 35-45 kD molecular weight range while vicilin appeared in the molecular weight range of 55-75 kD.

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Cowpea seed protein hydrolysates (CPH) were output from cowpea seeds applying alcalase from . CPH with an elevated level of hydrolysis was fractionated by size exclusion chromatography (SEC). Both CPH and SEC-portions showed to contain antimicrobial peptides (AMPs) as they inhibited both Gram-positive bacteria, such as LMG10470 , .

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Lettuce () was grown using a foliar spray with whey protein hydrolysate (WPH) as opposed to normal nitrate fertilization. Lettuce juice was prepared from lettuce cultivated without any fertilization, nitrate fertilization, or WPH. Sixty weaned, 4-week-old male V-line rabbits with an average 455 ± 6 g body weight were randomly divided into 4 groups ( = 15) and administered different lettuce juices.

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