Molecular mechanism of NAD and NMN binding to the Nudix homology domains of DBC1.

Deleted in breast cancer 1 (DBC1) is a human nuclear protein that modulates the activities of various proteins involved in cell survival and cancer progression. Oxidized form of nicotinamide adenine dinucleotide (NAD) is suggested to bind to the Nudix homology domains (NHDs) of DBC1, thereby regulating DBC1-Poly (ADP-ribose) polymerase 1 (PARP1) interactions, resulting in the restoration of DNA repair. Using Nuclear Magnetic Resonance (NMR) and Isothermal Titration Calorimetry (ITC), we confirmed NAD and its precursor nicotinamide mononucleotide (NMN) both bind the NHD domain of DBC1 (DBC1).

Different Influences of Rare Earth Eu Addition on Primary Si Refinement in Hypereutectic Al-Si Alloys with Varied Purity.

The effect of alloying the Eu element on primary Si refinement in varied purity Al-16Si alloys was studied by scanning electron microscopy (SEM), thermal analysis, micro x-ray diffraction (μ-XRD), electron probe microanalysis (EPMA), and transmission electron microscopy (TEM). The results indicate that the P impurity element in hypereutectic Al-Si alloys has a great influence on the rare earths' refinement efficiency of primary Si. Coinstantaneous primary Si refinement and eutectic Si modification by Eu was obtained in high purity (HP) Al-16Si and commercial purity (CP) Al-16Si-0.

Development of a lateral flow immunochromatographic assay for rapid detection of Mycoplasma pneumoniae-specific IgM in human serum specimens.

Early diagnosis of Mycoplasma pneumoniae (MP) infection is crucial for prompt treatment and good patient outcome. However, serological tests to detect MP rapidly and conveniently are still lacking. This study aimed to use the fluorescent dye Alexa Fluor® 647 as the detection marker to develop a lateral flow immunochromatographic assay (LFIA) for detection of MP-specific IgM in serum specimen.

Relationships of mental health problems with stress among civil servants in Guangzhou, China.

Psychosocial problems and stress-related diseases account for a high proportion of health problems. This study aimed to assess stress status and mental health, and their relationships with each other among civil servants in China. In a cross-sectional study of 600 randomly selected civil servants in 2007, we assessed mental health via the Symptom Checklist 90 (SCL-90), and stress status via a Self-Rated Stress Scale.

Association of somatic symptoms with depression and anxiety in clinical patients of general hospitals in Guangzhou, China.

Objectives: In high-income countries, depression and anxiety are reported to be common reasons for patients visiting nonpsychiatric services. This study aimed to assess the rate of depression and anxiety, and their associations with somatic symptoms, in patients presenting to clinics of general hospitals in Guangzhou, China.

Methods: In a hospital-based cross-sectional study of 2408 randomly selected clinical patients from 15 general hospitals, we assessed depression and anxiety via the Hospital Anxiety and Depression Scale (HADS), somatic symptoms via the Patient Health Questionnaire 15-Item Somatic Symptom Severity Scale (PHQ-15) and patients' view of the impact of somatic symptoms on their life, job and social relationships.