Drivers of anoxia in a eutrophic lagoon with clams farming (Sacca di Goro Lagoon, Italy): risk evaluation via multivariate statistics and timescales analysis.

The anoxia risk in a shallow coastal lagoon (Sacca di Goro (SG), Italy) experiencing multiple pressures (clam aquaculture, anthropogenic nutrient inputs, climate change) was assessed by combining timescales of anoxia onset, benthic nutrient turnover, horizontal and vertical transport, multivariate statistics, and Geographic Information System (GIS). The biogeochemical and transport timescales were obtained by experimental measurements and physical modeling. To this purpose, daily oxygen (O) and nutrient fluxes [dissolved inorganic phosphorus (DIP), ammonium (NH), dissolved reactive silica (SiO)] were measured in the benthic and pelagic compartments at six areas of the lagoon catching its complex spatial heterogeneity due to riverine and marine inputs, clams farming, depths, flushing and vertical exchange times, amount and quality of organic matter.

Leveraging Active Learning to Establish Efficient In Vitro Transcription and Translation from Bacterial Chromosomal DNA.

Gene expression is a fundamental aspect in the construction of a minimal synthetic cell, and the use of chromosomes will be crucial for the integration and regulation of complex modules. Expression from chromosomes in vitro transcription and translation (IVTT) systems presents limitations, as their large size and low concentration make them far less suitable for standard IVTT reactions. Here, we addressed these challenges by optimizing lysate-based IVTT systems at low template concentrations.