A Mixed-Linkage-Selective β-1,4-Glucanase from : Functional Characterization and Mechanistic Insights into Substrate Cleavage Specificity.

Macrofungal β-glucanases hold promise for the targeted bioconversion of dietary polysaccharides, yet their functional diversity and mechanistic specificity remain limited. In this study, a β-1,4-glucanase (PTRBGL4) was identified from genome via CAZy and dbCAN3-guided mining and heterologously expressed in . Biochemical assays revealed that PTRBGL4 exhibits low activity toward typical β-1,4-linked substrates, but demonstrates high specificity for mixed-linked β-1,3-1,4-glucans, including oat and barley β-glucans.

Influence of antidepressants on stream microbiota: Consequences for leaf itter breakdown and nutrient cycling.

As an emerging pollutant, antidepressants in wastewater have received extensive attention due to their metabolic stability and antimicrobial activity in aquatic systems. However, the scarcity of experimental data limits the validation of their potential impacts on ecosystem functionality. This study examined the effects of fluoxetine and amitriptyline (0-100 ng L) on microbial decomposers in stream ecosystems.

Polystyrene size-dependent impacts on microbial decomposers and nutrient cycling in streams.

The particle size of plastic is one of the most important factors influencing its ecotoxicity, but we are unclear about the effect of polystyrene (PS) particle size on microbial decomposers and consequent nutrient cycling in streams. Here, using microcosm experiments, we assessed how three PS sizes (50 nm, 1 μm, and 20 μm) influenced the process and consequences of leaf litter decomposition. Under acute exposure to 1 μm and 20 μm PS, fungal biomass significantly decreased, but microbial biomass significantly increased, indicating compensations may work between fungi and other microbial decomposers.