Improvements in the Duckweed-Microbe Co-cultivation Method for the Stable and Efficient Isolation of Rarely Cultivated Bacteria Using Microfilter Membranes.

We recently proposed a novel microbial isolation technique, the "duckweed-microbe co-cultivation method", for isolating a wide variety of microbes, including rarely cultivated microbes. This method involves the inoculation of aseptic duckweed with environmental microbes followed by co-cultivation for a set period. Plants and their surrounding medium are then used as microbial sources for isolation in the conventional agar plate method.

Mechanisms of histopathologic vascular damage caused by pancreatic juice leakage: Implications for preventing hemorrhagic complications.

Background: This study presents a prospective experimental analysis aimed to investigate the enzymatic interactions that cause histologic changes in the vascular wall to help prevent bleeding complications after pancreatic surgery. Severe hemorrhage can result from the accumulation of pancreatic juice and digestive fluids around vascular stumps. However, the specific digestive enzymes and their interactions that cause histologic damage to vascular walls remain unclear.

Mechanochemical Synthesis of H Materials: Hydrogen-Rich Perovskite Oxyhydrides with Lattice Strain as an Ammonia Synthesis Catalyst.

Mixed-anion perovskite compounds containing hydride ions (H) are promising catalysts for ammonia synthesis under mild reaction conditions. Oxyhydride BaTiOH is a typical example, with a positive correlation between H concentration and catalytic activity; however, the previously reported topochemical synthesis achieved a maximum value of ∼0.6.

Exploration of Lithium-Ion Conductors Based on Local Coordination Environments Using Crystallographic Site Fingerprints.

The development of high-performance solid-state electrolytes for Li-ion batteries represents a critical challenge because many potential Li-containing compounds remain unexplored. In order to overcome this challenge, in this study, we utilized a semisupervised learning approach to streamline the discovery of novel Li-ion conductors by focusing on local coordination environments. Herein, we introduced four structure-representation descriptors to represent local coordination and applied agglomerative clustering to a data set of 3,835 Li-containing structures.

[A Case of Achieving Radical Cure While Preserving the Function of the Pancreatic Body in a Patient with Double Primary Tumors of Pancreatic Head Cancer and Pancreatic Tail NEN].

While pancreatic ductal adenocarcinoma (PDAC) originates from exocrine cells of the pancreas, pancreatic neuroendocrine neoplasms (PNENs) arise from pancreatic neuroendocrine cells. The concurrent presence of an exocrine pancreatic tumor and a pancreatic neuroendocrine tumor is referred to broadly as a pancreatic collision tumor. In cases where the lesions are spatially distant, they are termed as multiple primary tumors, a relatively uncommon occurrence.

A trade-off between migration and association energies for hydride-ion conductivity in the SrLiH-CaLiH-NaLiH system.

Hydride-ion (H) conductors have garnered much attention owing to their high ionic conductivity and potential applications such as batteries and fuel/electrolysis cells. Perovskite-type H conductors are known to exhibit relatively high ionic conductivity at room temperature. The present work demonstrated systematic material exploration within the SrLiH-CaLiH-NaLiH pseudo-ternary system.

LiNbHO: a new transition-metal oxyhydride with rock-salt-type structure.

Transition-metal oxyhydrides are an emerging class of functional materials; however, the known compounds have mostly been limited to perovskite-type sturctures. Here, we successfully synthesized LiNbHO, the first example of a rock-salt-type transition-metal oxyhydride, using mechanochemical methods. Galvanostatic charge/discharge tests revealed that LiNbHO functions as an electrode for lithium secondary batteries.

Crystal and Local Structures of PrCoD during Its Deuterium Absorption Process Determined by Neutron Diffraction.

We investigated crystal and local structures of PrCo, PrCoD and PrCoD by neutron diffraction. The determined structural model of PrCoD was the CeNi-type structure by Rietveld refinement, where the deuterium atoms occupied both the CaCu-type (I) and the MgZn-type cells. The deuterium contents in these cells were 0.

Successful Isolation of Diverse Verrucomicrobiota Strains through the Duckweed-Microbes Co-cultivation Method.

The "duckweed-microbes co-cultivation method" is a microbial isolation technique that effectively recovers diverse microbes, including rarely cultivated bacterial phyla, from environmental samples. In this method, aseptic duckweed and microbes collected from an environmental sample are co-cultivated for several days, and duckweed-associated microbes are then isolated from its roots using a conventional agar plate-based cultivation method. We herein propose several improvements to the method in order to specifically obtain members of the rarely cultivated bacterial phylum, Verrucomicrobiota.

Reduced-port robotic pancreaticoduodenectomy with optimized surgical field deployment: early results of single-site plus-two ports method.

Background: The adoption of Robotic Pancreaticoduodenectomy (RPD) is increasing globally. Meanwhile, reduced-port RPD (RPRPD) remains uncommon, requiring robot-specific techniques not possible with laparoscopy. We introduce a unique RPRPD technique optimizing surgical field exposure.

Crystal structure, microstructure, and mechanical properties of heat-treated oyster shells.

We investigated the crystal structure and mechanical properties of oyster shells subjected to heat treatment under increasing temperature conditions. The shell contained folia and chalky layers. The folia layer comprised two CaCO phases: 72.

A pediatric case of infliximab-resistant ulcerative colitis successfully treated using vedolizumab.

Pediatric ulcerative colitis is likely to be more severe than adult ulcerative colitis. Failure to thrive should be considered during therapy. A 10-year-old boy was diagnosed with ulcerative colitis based on his clinical presentation and colonoscopy and biopsy results.

Age-related change in mammographic breast density of women without history of breast cancer over a 10-year retrospective study.

Background: Women with higher breast density are at higher risk of developing breast cancer. Breast density is known to affect sensitivity to mammography and to decrease with age. However, the age change and associated factors involved are still unknown.

Complete Genome Sequence of sp. Strain TBR-22.

We report a complete genome sequence of a novel bacterial isolate, strain TBR-22, belonging to the class of the phylum , which was isolated from duckweed fronds. The genome expands our knowledge of the lifestyle of this abundant but rarely characterized phylum.

Draft Genome Sequence of Strain F-183.

Here, we report a draft genome sequence of a bacterial strain, F-183, isolated from a duckweed frond. Strain F-183 belongs to the family of the phylum , and its genomic information would contribute to understanding the ecophysiology of this abundant but rarely characterized phylum.

Microalgal transformation of food processing byproducts into functional food ingredients.

Large amounts of food processing byproducts (FPBs) are generated from food manufacturing industries, the second-largest portion of food waste generation. FPBs may require additional cost for post-treatment otherwise cause environmental contamination. Valorization of FPBs into food ingredients by microalgae cultivation can save a high cost for organic carbon sources and nutrients from medium cost.

Growth Promotion of Giant Duckweed (Lemnaceae) by sp. SP4 Through Enhancement of Nitrogen Metabolism and Photosynthesis.

Duckweeds (Lemnaceae) are representative producers in fresh aquatic ecosystems and also yield sustainable biomass for animal feeds, human foods, and biofuels, and contribute toward effective wastewater treatment; thus, enhancing duckweed productivity is a critical challenge. Plant-growth-promoting bacteria (PGPB) can improve the productivity of terrestrial plants; however, duckweed-PGPB interactions remain unclear and no previous study has investigated the molecular mechanisms underlying duckweed-PGPB interaction. Herein, a PGPB, sp.

Isolation and Characterization of -Associated Bacteria, sp. CA3 and sp. CN5, Capable of Promoting the Growth and Paramylon Production of under Mixotrophic Cultivation.

produces paramylon, which is a feedstock for high-value functional foods and nutritional supplements. The enhancement of paramylon productivity is a critical challenge. Microalgae growth-promoting bacteria (MGPB) can improve microalgal productivity; however, the MGPB for remain unclear.

Novel Plant-Associated Promotes Growth of Common Floating Aquatic Plants, Duckweeds.

Duckweeds are small, fast growing, and starch- and protein-rich aquatic plants expected to be a next generation energy crop and an excellent biomaterial for phytoremediation. Despite such an importance, very little is known about duckweed-microbe interactions that would be a key biological factor for efficient industrial utilization of duckweeds. Here we first report the duckweed growth promoting ability of bacterial strains belonging to the phylum , the members of which are known to inhabit soils and terrestrial plants, but their ecological roles and plant-microbe interactions remain largely unclear.

Comparative Analysis of Microbial Communities in Fronds and Roots of Three Duckweed Species: Spirodela polyrhiza, Lemna minor, and Lemna aequinoctialis.

The microbial communities inhabiting the fronds of duckweeds have not been investigated in as much detail as those on the roots. We herein examined the microbial communities in three duckweed species using 16S rRNA amplicon sequencing and compared them to those on the roots. The microbial compositions of the fronds were distinct from those of the roots in the three species.

Enhanced lipid productivity of Chlamydomonas reinhardtii with combination of NaCl and CaCl stresses.

Salinity (NaCl) stress treatment is a strategy to induce lipid accumulation in microalgae. This study aimed to investigate the effect of a combination of two salts (NaCl/CaCl) on lipid productivity of Chlamydomonas reinhardtii. C.

Enhanced production of biomass and lipids by via co-culturing with a microalga growth-promoting bacterium, sp. EG3.

Background: , a unicellular flagellated microalga, is regarded as one of the most promising species as microalgal feedstock for biofuels. Its lipids (mainly wax esters) are suitable for biodiesel and jet fuel. Culture of using wastewater effluent will improve the economics of biofuel production.