Recent progress in the identification and in vitro culture of skin organoids.

An organoid is a cell-based structure that shows organ-specific properties and shares a similar spatial organization as the corresponding organ. Organoids possess powerful capability to reproduce the key functions of the associated organ structures, and their similarity to the organs makes them physiologically relevant systems. The primary challenge associated with the development of skin organoids is the complexity of the human skin architecture, which encompasses the epidermis and the dermis as well as accessory structures, including hair follicles, sweat glands, and sebaceous glands, that perform various functions such as thermoregulation.

Optimal Formula of Angelica sinensis Ameliorates Memory Deficits in β-amyloid Protein-induced Alzheimer's Disease Rat Model.

Objective: Angelica (A.) sinensis is used as a traditional medical herb for the treatment of neurodegeneration, aging, and inflammation in Asia. A.

Hydrogen gas protects against delayed encephalopathy after acute carbon monoxide poisoning in a rat model.

: The protective effects of 2%-4% hydrogen gas in delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) have been previously reported. This study aimed to assess the neuroprotective effects of high concentration hydrogen (HCH) on DEACMP.: A total of 36 male Sprague-Dawley rats were divided into 3 groups.

Corneal Biomechanical Evaluation After Conventional Corneal Crosslinking With Oxygen Enrichment.

Purpose: To assess corneal biomechanical changes after conventional corneal crosslinking (CXL), with and without oxygen enrichment.

Methods: Sixty fresh porcine corneas were randomly divided into group 1 (control), group 2 (conventional CXL), and group 3 (conventional CXL in a high-oxygen environment during ultraviolet A [UVA] irradiation). After crosslinking, a 5-mm wide corneal strip was extracted using a double-bladed knife from 12 to 6'o clock.

IGFBP7 regulates sepsis-induced epithelial-mesenchymal transition through ERK1/2 signaling.

The epithelial-mesenchymal transition (EMT) process results in fibrosis of renal tubular epithelial cells and is of great importance in the development of acute kidney injury (AKI). Urinary IGF-binding protein-7 (IGFBP7) was obviously increased in AKI and is considered to be a biomarker for AKI. However, whether it has an effect on the inhibition of lipopolysaccharide (LPS)-induced EMT in human HK2 cells and on that of cecal ligation and puncture (CLP)-induced EMT in human HK2 cells and in mice remains to be elucidated.

Effects of CXCL4/CXCR3 on the lipopolysaccharide-induced injury in human umbilical vein endothelial cells.

Chemokines and inflammatory response of endothelial cells is crucial in the development and progression of inflammatory disease. Lipopolysaccharide (LPS) is a well-known factor to trigger inflammatory response and induce damage of endothelial cells. The present study used lipopolysaccharide (LPS)-treated human vascular endothelial cells (HUVECs) to investigate the function of chemokine CXC chemokine ligand 4 (CXCL4) and its receptor CXC chemokine receptor 3 (CXCR3) in inflammatory-induced endothelial injury.

Neuroprotective effects of methane-rich saline on experimental acute carbon monoxide toxicity.

Background: Methane has been reported to play a protective role in ischemia-reperfusion injury via anti-oxidation, anti-inflammatory and anti-apoptotic activities. This study was designed to determine the protective effects of methane-rich saline (MRS) on acute carbon monoxide (CO) poisoning.

Methods: A total of 36 male Sprague-Dawley rats were randomly divided into 3 groups: sham group, CO group and MRS group.