Transcriptome mapping of unfertilized pea flowers under high temperature: insights into gene regulatory networks genomics resources.

Heat stress is the most significant environmental constraint on pea production, and heat tolerance mechanisms are mediated through a variety of pathways. Pea (Pisum sativum L.) cultivar Arka Chaitra (AC) is considered a heat tolerant variety, whereas cultivar Matar Ageta (MA) is heat sensitive.

Prevalence, antimicrobial resistance and biofilm forming ability of in milk, animal handlers and slurry samples from dairy herds of Punjab, India.

Antimicrobial-resistant serves as an indicator for monitoring the occurrence of antimicrobial resistance (AMR) at the human, animal, and environmental interface. The present study employs a 'One Health' framework to determine the prevalence, phenotypic and genotypic characterisation of the AMR profile and biofilm forming ability of isolates from milk, hand swabs and slurry samples of 405 dairy herds in Punjab, India. An overall prevalence of was 34.

Automated framework for comprehensive usability analysis of healthcare websites using web parsing.

In the digital age, hospital websites are essential for providing healthcare information and services. This research introduces an automated tool, WUAHP, created in Python utilizing BeautifulSoup for HTML parsing. This facilitates the extraction of structural and content-based components essential for usability assessment.

Comparative analysis of semantic-segmentation models for screen film mammograms.

Accurate segmentation of mammographic mass is very important as shape characteristics of these masses play a significant role for radiologist to diagnose benign and malignant cases. Recently, various deep learning segmentation algorithms have become popular for segmentation tasks. In the present work, rigorous performance analysis of ten semantic-segmentation models has been performed with 518 images taken from DDSM dataset (digital database for screening mammography) with 208 mass images ϵ BIRAD3, 150 mass images ϵ BIRAD4 and 160 mass images ϵ BIRAD5 classes, respectively.

Nutrient and Potentially Toxic Metal Concentrations in Wheat and Rice Fodder from the World Renowned Ramsar Site.

The present study was conducted to analyze the contents of various macronutrients, micronutrients and potentially toxic metals (PTMs) in wheat and rice shoot samples collected from villages around Harike wetland, India. In wheat shoots, levels of most macronutrients and in rice shoots levels of Na, S and P were found to be below the normal requirement levels. Among the micronutrients, the contents of Cu, Co, Fe and Mn were found to be higher and those of Se were lower than the normal requirement levels in both wheat and rice shoots.

Functional Outcome of Intraarticular Osteotomy and Osteosynthesis for Calcaneal Malunion with Salvageable Subtalar Joint: A Retrospective Case Series.

Background And Objective: Calcaneal malunion, a frequent complication of calcaneal fractures, disrupts hindfoot anatomy, causing pain, deformity, stiffness, and gait abnormalities. While conservative management can be effective in selected cases, surgical intervention is often required. This study evaluates the efficacy of intraarticular osteotomy and osteosynthesis as a joint-preserving procedure to manage calcaneal malunion and prevent subtalar arthrodesis.

Suspected Emphysematous Cellulitis of the Face Secondary to Untreated Nondisplaced Angle of the Mandible Fracture: A Case Report.

Facial cellulitis with palpable crepitus is a rare complication following a nondisplaced angle of mandible fracture. We report a case of a male in his mid-20s who presented with suspected emphysematous cellulitis of the face 3 days after an assault. Clinical examination revealed diffuse facial swelling with palpable subcutaneous crepitus over the left cheek and submandibular region.

Machine learning approach for optimizing usability of healthcare websites.

In today's digital era, hospital websites serve as crucial informational resources, providing patients with easy access to medical services. Ensuring the usability of these websites is essential, as it directly impacts users' ability to navigate and retrieve vital medical information. Despite the recognized importance of website usability in the healthcare sector, there is a notable lack of empirical studies leveraging machine learning to assess this usability.

Outcomes of Surgical Management of Late Presenting Intra-Articular Distal Femoral Physeal Fracture: A Multicentric Retrospective Case Series.

Introduction: Distal femur physeal fractures in children, particularly Hoffa fractures, are rare and prone to complications. This study aims to evaluate the outcomes of surgical intervention in children presenting with delayed intraarticular distal femur physeal fractures.

Materials And Methods: A multicentric retrospective study involving six pediatric patients with delayed presentation of distal femur physeal fractures was conducted.

A Protocol for High-efficiency Transformation and Genome Editing in Elite Wheat Cultivars.

CRISPR-Cas9-based editing offers a simple yet efficient method for introducing targeted mutations in the genes of interest. Low efficiency of transformation and a lack of reproducibility in editing genes in elite wheat cultivars are the bottlenecks in improving simply inherited traits. We describe a high-efficiency transformation protocol for editing elite wheat cultivars using JD633-GRF4-GIF1 CRISPR vector.

Study of radiation exposure to radon in groundwater using scintillation-based RnDuo technique: A statistical analysis for risk assessment.

Radon in household water, particularly from groundwater sources, is a well-known health concern. Radon in groundwater is typically produced by radium in the aquifer's rocks, which may be originated from geogenic uranium or be transported from distant areas. In this study, radon levels were measured for the first time in groundwater samples (N = 80) collected from the foothills of the Kumaun Himalaya using the scintillation-based RnDuo technique, with the aim to assess whether exposure to radon in the water poses a significant health risk to the general public.

Characterizing the Role of Endocannabinoid Receptor Cnr1 in Mouse Ovarian Granulosa Cells.

The endocannabinoid receptors Cnr1 and Cnr2 have been found in reproductive organs such as the oviduct and uterus. These receptors bind to endocannabinoids, the arachinodoylethanolamine (AEA) and arachinodoylglycerol (2-AG), respectively. Both cannbinoid receptors have been investigated for their role in implantation and fertilization.

Deep transfer learning based hierarchical CAD system designs for SFM images.

Present work involves rigorous experimentation for classification of mammographic masses by employing four deep transfer learning models using hierarchical framework. Experimental work is carried on 518 SFM images of DDSM dataset with 208, 150 and 160 images of probably benign, suspicious- malignant and highly malignant classes, respectively. ResNet50 model is used for generating segmented mass images.

The Scottish Retinal Detachment Study: 10-year outcomes after retinal detachment repair.

Objective: To address the paucity of long-term data on outcomes following rhegmatogenous retinal detachment (RRD) repair we aimed to establish the 10-year best corrected visual acuity (BCVA), redetachment rate and lens status for patients from the Scottish Retinal Detachment Study.

Subjects: Data from patients who presented with RRD during the original study were collected from clinical records 10 years after repair. Patients were excluded if lacking 10 year follow-up data, and excluded from visual acuity analysis in the case of significant co-morbid ophthalmic disease.

Occurrence, Multidrug Resistance, SCCmec Typing of Methicillin-Resistant Staphylococcus aureus from Farmed Eggs and Environment.

A comparative cross-sectional study was conducted on farmed eggs (n: 480) and environmental samples (n: 72; feed, water, and poultry droppings) from twenty-four deep litter and caged poultry layer farms across Punjab, India. The study noted a significantly higher occurrence of Staphylococcus aureus (31.67%; 95% CI 26.

Complications and blood loss after invasive treatments for small renal masses A systematic review.

Introduction: This systematic review and meta-analysis provides estimates of major complications and estimated blood loss (EBL) for open partial nephrectomy (OPN), conventional laparoscopic partial nephrectomy (LPN), and robot-assisted partial nephrectomy (RAPN). Additionally, it outlines the incidence of major complications associated with percutaneous thermal ablation (TA) in patients with small renal masses (SRMs).

Methods: We searched MEDLINE, EMBASE, and CINAHL from inception to the end of July 2023.

Banana and Guava dataset for machine learning and deep learning-based quality classification.

In the field of agriculture, the identification and classification of fruits have become necessary for sustainable growth in horticulture sectors. Recently, different types of advanced techniques have been used for the identification and classification of fruits. Machine learning is an emerging technology with robust application in different sectors that can be used for the classification of fruits.

Utilizing Short Interspersed Nuclear Element as a Genetic Marker for Pre-Harvest Sprouting in Wheat.

Pre-harvest sprouting (PHS) is a complex abiotic stress caused by multiple exogenous and endogenous variables that results in random but significant quality and yield loss at the terminal crop stage in more than half of the wheat-producing areas of the world. Systematic research over more than five decades suggests that addressing this challenge requires tools beyond the traditional genetic manipulation approach. Previous molecular studies indicate a possible role of epigenetics in the regulation of seed dormancy and PHS in crops, especially through RNA-directed DNA methylation (RdDM) pathways mediated by Argonaute (AGO) proteins.

Occurrence, Multidrug Resistance, and Multilocus Sequence Typing of Extended-Spectrum Beta-Lactamase/AmpC-Producing from Farmed Eggs.

The present study evaluated the occurrence, antibiogram profile, and sequence types (STs) of multidrug resistant (MDR) from freshly laid eggs ( = 480), feed ( = 24), water ( = 24), poultry droppings ( = 24), and hand swab samples ( = 10) collected from 24 deep litter (DL) and caged poultry layer farms (12 per category) across Punjab, India. The overall contamination rate in DL and cage farms was 32% (95% confidence intervals [CI], 26.6-37.

MARS and RNAcmap3: The Master Database of All Possible RNA Sequences Integrated with RNAcmap for RNA Homology Search.

Recent success of AlphaFold2 in protein structure prediction relied heavily on co-evolutionary information derived from homologous protein sequences found in the huge, integrated database of protein sequences (Big Fantastic Database). In contrast, the existing nucleotide databases were not consolidated to facilitate wider and deeper homology search. Here, we built a comprehensive database by incorporating the non-coding RNA (ncRNA) sequences from RNAcentral, the transcriptome assembly and metagenome assembly from metagenomics RAST (MG-RAST), the genomic sequences from Genome Warehouse (GWH), and the genomic sequences from MGnify, in addition to the nucleotide (nt) database and its subsets in National Center of Biotechnology Information (NCBI).