Inflammation awakens dormant cancer cells by modulating the epithelial-mesenchymal phenotypic state.

The awakening of dormant disseminated cancer cells appears to be responsible for the clinical relapses of patients whose primary tumors have been successfully cured months and even years earlier. In the present study, we demonstrate that dormant breast cancer cells lodged in the lungs reside in a highly mesenchymal, nonproliferative phenotypic state. The awakening of these cells is not triggered by a cancer cell-autonomous process.

Anethole addition during in vitro follicle culture and oocyte in vitro maturation improves cumulus-oocyte complexes quality and embryo production rates.

In vitro follicle culture (IVFC) supports the development of in vitro-grown cumulus-oocyte complexes (COCs), but oocyte maturation and embryo production rates from these COCs are often lower than those of in vivo-derived counterparts. Anethole, a natural antioxidant, has shown potential to improve follicle development and oocyte maturation in vitro. However, the optimal timing of anethole supplementation during IVFC and/or in vitro maturation (IVM), and its impact on developmental outcomes relative to in vivo-derived COCs, remains to be clarified.

The efficacy of trans-anethole supplementation during in vitro culture of follicles, oocytes and embryos: A meta-analysis approach.

This study aimed to evaluate the effects of trans-anethole supplementation on in vitro reproductive outcomes using a meta-analysis approach. A total of 128 records were retrieved from four major scientific databases. After screening and eligibility assessment, 6 studies were included in the final analysis.

A specific, stable, and accessible LAMP assay targeting the HSP70 gene of .

Diagnostic delays prevent most Chagas disease patients from receiving timely therapy during the acute phase when treatment is effective. qPCR-based diagnostic methods provide high sensitivity during this phase but require specialized equipment and complex protocols. More simple and cost-effective tools are urgently needed to optimize early Chagas disease diagnosis in low-income endemic regions.

SLO co-opts host cell glycosphingolipids to access cholesterol-rich lipid rafts for enhanced pore formation and cytotoxicity.

Unlabelled: Streptolysin O (SLO) is a virulence determinant of group A (), the agent of streptococcal sore throat and severe invasive infections. SLO is a member of a family of bacterial pore-forming toxins known as cholesterol-dependent cytolysins, which require cell membrane cholesterol for pore formation. While cholesterol is essential for cytolytic activity, accumulating data suggest that cell surface glycans may also participate in the binding of SLO and other cholesterol-dependent cytolysins to host cells.