Mechanistic evaluation of Clerodendrum serratum anti-biofilm potency against Mycobacterium species.

Biofilm formation in Mycobacterium species significantly contributes to their pathogenicity and resistance to conventional antimicrobial therapies, posing a major challenge in clinical management. Plant-derived phytoconstituents have emerged as promising alternatives due to their diverse biological activities, including anti-biofilm properties. Clerodendrum serratum, a medicinal plant known for antimicrobial potential, offers a rich source of such bioactive compounds.

Correlation between wastewater-based substance use prevalence and syringe distribution in a harm reduction program in the United States.

A comprehensive, near-real-time assessment of substance use can offer critical insights to -improve the effectiveness of current harm reduction strategies aimed at preventing overdose fatalities. This study employed wastewater-based epidemiology to monitor psychostimulants, opioids, medications for opioid use disorder, antipsychotics, antidepressants, and cannabis over 12 months in a community operating a syringe services program -. On average, an estimated 44,000 monthly doses of methamphetamine (2660 ± 547 mg/d/1000 people), 41,600 doses of oxycodone (159 ± 109 mg/d/1000 people), 9600 doses of cocaine (688 ± 240 mg/d/1000 people), and 19,000 doses of venlafaxine (242 ± 47.

Synergistic efficacy of phages along with vancomycin for eradication of vancomycin-resistant biofilms.

Background: The upsurge of antibiotic resistance is a significant challenge to public health, and the dry pipeline of new antibiotics has prompted the discovery of alternative treatment approaches. () isolates are often multidrug-resistant, posing challenges to antibiotic therapy. Bacteriophage therapy is being explored as an alternative method to treat the growing population of antibiotic-resistant infections.

Induced antibiotic production against pathogenic Klebsiella pneumoniae by bacterial coculture.

The world is running short of effective antibiotics due to rapid development of antibiotic resistance against currently available antibiotics. The frequency of new antibiotic discovery from conventional axenic cultures has decreased significantly. In current study, azithromycin and ciprofloxacin supplemented plates were used as selection filter for isolation of antibiotic-producing bacteria from Gomti river sediments.

Exploring anticancer, antioxidant, and antimicrobial potential of , a fungal endophyte isolated from leaf callus.

Background: Endophytic fungi represent a compelling assemblage of microorganisms that inhabit plant tissues without inflicting any discernible detriment to the host organism. They foster a symbiotic association with their host plants, frequently conferring advantages such as augmented growth, enhanced resilience to stressors, and safeguarding against pathogens.

Study Design: is a medicinal tree of Dilleniaceae.

Evaluation of bacteriophage cocktail on urinary tract infection caused by colistin-resistant Klebsiella pneumoniae in mice model.

Objective: The colistin-resistant Klebsiella pneumoniae causes complicated urinary tract infections (UTIs). Of them, 73% of strains of K. pneumoniae formed moderate to strong biofilm.

A Randomized, Placebo-controlled, Double-blind Clinical Trial of Bacteriophage Cocktails in Chronic Wound Infections.

Background: Chronic wounds are prevalent globally at endemic proportions. The common features associated with chronic wounds are prolonged inflammatory phase, infection with multidrug-resistant (MDR) bacteria, and subsequent biofilm formation. The present randomized-controlled trial (RCT) study was undertaken on chronic wounds of ≥6 weeks longer duration using customized phages to evaluate the efficacy and safety of bacteriophage therapy.

Phage therapy: a revolutionary shift in the management of bacterial infections, pioneering new horizons in clinical practice, and reimagining the arsenal against microbial pathogens.

The recent approval of experimental phage therapies by the FDA and other regulatory bodies with expanded access in cases in the United States and other nations caught the attention of the media and the general public, generating enthusiasm for phage therapy. It started to alter the situation so that more medical professionals are willing to use phage therapies with conventional antibiotics. However, more study is required to fully comprehend phage therapy's potential advantages and restrictions, which is still a relatively new field in medicine.

Bacteriophage entrapped chitosan microgel for the treatment of biofilm-mediated polybacterial infection in burn wounds.

Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) bacteria are most commonly present in burn wound infections.

A Bacteriophage-Loaded Microparticle Laden Topical Gel for the Treatment of Multidrug-Resistant Biofilm-Mediated Burn Wound Infection.

Klebsiella pneumoniae is regarded as one of the most profound bacteria isolated from the debilitating injuries caused by burn wounds. In addition, the multidrug resistance (MDR) and biofilm formation make treating burn patients with clinically available antibiotics difficult. Bacteriophage therapy has been proven an effective alternative against biofilm-mediated wound infections caused by MDR bacterial strains.

A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Bacterial Infections in Burn Wounds.

Multidrug-resistant (MDR) () is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the -specific bacteriophages (BPABΦ1), loaded into the chitosan microparticles followed by dispersion in gel, and evaluated therapeutic efficacy against MDR clinical strains.

A novel approach for rapid and sensitive detection of Zika virus utilizing silver nanoislands as SERS platform.

To control the spread of the disease, the Zika virus (ZIKV), a flavivirus infection spread by mosquitoes and common in across the world, needs to be accurately and promptly diagnosed. This endeavour gets challenging when early-stage illnesses have low viral loads. As a result, we have created a biosensor based on surface-enhanced Raman scattering (SERS) for the quick, accurate, and timely diagnosis of the Zika virus.

Bacteriophage therapy for -induced urinary tract infection in rats.

The present study evaluates the efficacy of bacteriophage therapy for urinary tract infection (UTI) in rats. UTI was established by inoculating (100 μl) at a concentration of 1.5 × 10 CFU/ml per urethra via a cannula in different groups of rats.

Effect of four-layer dressing on the microbiological profile of venous leg ulcer.

Objective: Venous leg ulcer (VLU) is a chronic disease and has periods of exacerbation and remission. Various bandage systems-single-layered, double-layered and multiple-layered with elastic and non-elastic components-have been developed. The requirement for sustained pressure brought about the introduction of the four-layer bandage.

Bacteriophages Can Make a Difference in Water Quality: Evidence From a Community-Based Study From North India.

Background and objectives Diarrhoea is a preventable and treatable faecal-oral disease. Despite significant inputs from the health and non-health sector in the treatment and prevention of diarrhoea, it remains a significant contributor to under-five-years children mortality and exerts profound effects on their growth and development. Bacteriophage has the potential to prevent diarrhoea.

SERS based rapid and ultrasensitive detection of Japanese Encephalitis Virus.

Japanese encephalitis (JE) is a mosquito-borne flavivirus infection named Japanese Encephalitis Virus (JEV), prevalent in Asia-pacific countries, requires an accurate and rapid diagnosis to contain the outbreak of the disease. In cases of low viral load in early-stage infections, this task becomes difficult. Therefore, we have developed a surface-enhanced Raman spectroscopy (SERS) based biosensor for rapid, sensitive, and early-stage detection of JE antigen.

Immunological and safety profile of bacteriophage therapy: A pre-clinical study.

Aims: Numerous pre-clinical and clinical studies have recently demonstrated the significant role of phage therapy in treating multidrug-resistant bacterial infections. However, only a few researchers have focused on monitoring the phage-mediated adverse reactions during phage therapy. The present study aimed to demonstrated the oral acute and sub-acute toxicity of bacteriophages (Klebsiella pneumoniae XDR strain) in Charles Foster rats with special reference to immunological response and adverse effects.

Evaluation of Bacteriophage Cocktail on Septicemia Caused by Colistin-Resistant in Mice Model.

The emergence of resistance against last-resort antibiotics, carbapenem and colistin, in has been reported across the globe. Bacteriophage therapy seems to be one of the most promising alternatives. This study aimed to optimize the quantity and frequency of bacteriophage cocktail dosage/s required to eradicate the bacteria in immunocompetent septicemic mice.