Production and Extraction of Red Bioactive Pigment from and Formulation of Bio-Lip Balm.

The current study is focused on the screening and isolation of a bioactive pigment producing actinomycete from paddy fields of Kerala, India. Among the five-pigment producing isolates obtained, a red colour pigmented actinomycetes was selected for further extraction and analysis. Optimization of production media enhanced the production of pigmented biomass.

Chitosan coated ZnO/Tio/GdO nano composites for improved rapamycin delivery in non-small cell lung cancer.

The increasing prevalence of Non-Small Cell Lung Cancer (NSCLC) demands innovative therapeutic strategies. Rapamycin, an mTORC1 inhibitor, shows promise in cancer therapy but is limited by drug resistance and low bioavailability. This study develops a novel rapamycin-loaded chitosan-coated ZnO/Tio/GdO nano-composite (RNC) incorporating ZnO, TiO₂, and Gd₂O₃ nanoparticles, coated with chitosan, to enhance rapamycin delivery and efficacy against A549 cancer cells.

Exploring the interactions of rapamycin with target receptors in A549 cancer cells: insights from molecular docking analysis.

Rapamycin, a macrocyclic antibiotic derived from the actinomycetes Streptomyces hygroscopicus, is a widely used immunosuppressant and anticancer drug. Even though rapamycin is regarded as a multipotent drug acting against a broad array of anomalies and diseases, the mechanism of action of rapamycin and associated pathways have not been studied and reported clearly. Also reports on the binding of rapamycin to cancer cell receptors are limited to the serine/threonine protein kinase mTORC1.

Molecular and therapeutic insights of rapamycin: a multi-faceted drug from Streptomyces hygroscopicus.

The advancement in pharmaceutical research has led to the discovery and development of new combinatorial life-saving drugs. Rapamycin is a macrolide compound produced from Streptomyces hygroscopicus. Rapamycin and its derivatives are one of the promising sources of drug with broad spectrum applications in the medical field.

Regulation of Runx2 and Its Signaling Pathways by MicroRNAs in Breast Cancer Metastasis.

As a major threat among women globally, breast cancer (BC) emerges as a primary research focus for several researchers. Although various therapeutic regimens are available, there is an increased chance of metastasis of BC cells, which raises the severity of this malignancy. Of multiple preferred secondary targets, metastasis to bone is extensively studied.

Assessment of monoamine neurotransmitters in the cortex and cerebellum of gamma-irradiated mice: A neuromodulatory role of .

Introduction: Radiation is an important tool in the diagnostic and curative treatment of many cancers. Ionizing radiation induces many biochemical changes in the cells. The present study was designed to estimate the level of neurotransmitters in the distinct brain tissue of Swiss albino mice before exposing gamma radiation.

An osteoinductive effect of phytol on mouse mesenchymal stem cells (C3H10T1/2) towards osteoblasts.

In recent years, phytochemicals have been widely researched and utilized for the treatment of various medical conditions such as cancer, cardiovascular diseases, age-related problems and are also said to have bone regenerative effects. In this study, phytol (3,7,11,15-tetramethylhexadec-2-en-1-ol), an acyclic unsaturated diterpene alcohol and a secondary metabolite derived from aromatic plants was investigated for its effect on osteogenesis. Phytol was found to be nontoxic in mouse mesenchymal stem cells (C3H10T1/2).

Defect engineering, microstructural examination and improvement of ultrafast third harmonic generation in GaZnO nanostructures: a study of e-beam irradiation.

Electron beam induced effects on defect engineering and structural, morphological and optical properties of Ga doped ZnO (GaZnO) nanostructures for improved ultrafast nonlinear optical properties are presented. A microstructural analysis was carried out based on the Scherrer, Williamson-Hall, and size-strain models. All three models reveal a peak broadening effect upon electron beam irradiation (EBI) and the crystallite size of the films shows a decrease of 30% compared to unirradiated nanostructures.

Modified Thermal, Dielectric, and Electrical Conductivity of PVDF-HFP/LiClO Polymer Electrolyte Films by 8 MeV Electron Beam Irradiation.

The polymer electrolyte films (poly((vinylidene fluoride)--hexafluoropropylene)/LiClO@90:10 w/w, PHL10) were prepared by solution-casting technique and the effect of various dosages of electron beam (EB) irradiation on structure, morphology, thermal, dielectric, and conductivity properties at various dosages. The atomic force microscope topography image shows substantial change in surface morphology due to irradiation and the modification of chemical bonds through chain scission process with increased EB dose was confirmed by Fourier transform infrared spectroscopy studies. NMR studies confirm the change in structural properties due to irradiation.

Inhibition of ERK1/2 or AKT Activity Equally Enhances Radiation Sensitization in B16F10 Cells.

Background: The aim of the study was to evaluate the radiation sensitizing ability of ERK1/2, PI3K-AKT and JNK inhibitors in highly radiation resistant and metastatic B16F10 cells which carry wild-type and .

Methods: Mouse melanoma cell line B16F10 was exposed to 1.0, 2.

Anticlastogenic, radiation antagonistic, and anti-inflammatory activities of in albino Wistar rat model.

The present study was undertaken to evaluate the anti-inflammatory activity and antigenotoxic effect of hydroalcoholic leaf extract of () in albino Wistar rats against whole body X-ray irradiation. Rats were orally administered with (25, 50, 100, 200, and 400 mg/kg body weight) of leaf extract for five days. On the fifth day after last administration, animals were exposed to whole body X-rays of 8 Gy.

Evaluation of the Potency of Kinetin on Radiation Induced Behavioural Changes in Swiss Albino Mice.

Introduction: According to the various independent studies conducted, it is well evident fact that radiation induces oxidative stress in the living system. It is also proved that this oxidative stress will lead to the various behavioural changes such as anxiety and memory impairment. Kinetin is one of the important plant cytokine with anti-aging properties.

NATURAL RADIONUCLIDE LEVELS IN SEDIMENTS OF CAUVERY RIVERINE ENVIRONMENT.

The paper presents the systematic measurement of activity concentrations of K, Ra and Th in sediment sample of Cauvery river of Karnataka, South India. Activity was measured using HpGe gamma ray spectrometer, and the mean values of K, Ra and Th in the sediment samples were found to be 144.31, 32.

Effect of electron-beam irradiation on antimicrobial, antibiofilm activity, and cytotoxicity of mouth rinses.

Background: Oral health diseases are common in all regions of the world. Mouth rinses are widely used generally by population as a port of daily oral care regimen. In addition to antimicrobial activity, mouth rinses possess certain cytotoxic effects.

Functional Genomic Investigation of the Molecular Biological Impact of Electron Beam Radiation in Lymphoma Cells.

Purpose: The biological response of electron beam radiation (EBR) in tumors remains underexplored. This study describes the molecular biological and genomic impact of EBR on tumor cells.

Methods: A mouse model bearing Dalton's lymphoma ascites cells was exposed to an 8-MeV pulsed electron beam, at a dose rate of 2 Gy/min using a microtron, a linear accelerator.

Haematopoietic, Antioxidant and Membrane Stabilizing Property of Diallyl Disulphide in Irradiated Mice.

Introduction: Diallyl disulphide is an organo-sulphur compound which is present in garlic and responsible for the characteristic odor of garlic. It is known for its anticancer and invitro membrane stabilizing properties.

Aim: The main aim was to evaluate the haematopoietic, antioxidant and membrane stabilizing property of diallyl disulfide in irradiated mice.

DOSE-RESPONSE STUDY USING MICRONUCLEUS CYTOME ASSAY: A TOOL FOR BIODOSIMETRY APPLICATION.

The present study is aimed at obtaining in vitro dose-response data for the induction of micronucleus (MN) and nucleoplasmic bridges (NPBs) in human lymphocytes using 60Co-gamma rays and 8 MeV pulsed electron beam. An attempt was made to validate the possibility of applying NPBs as new biodosimetry endpoint in the dose range of 0-6 Gy. A total of 1000 binucleated cells (BNCs) per dose point were evaluated for the frequency of MN and NPBs.

Changes in spectrochemical and catalytic properties of biopolymer anchored Cu(II) and Ni(II) catalysts by electron beam irradiation.

Chitosan (a biopolymer) anchored Cu(II) and Ni(II) Schiff base complexes, [M(OIAC)Cl2] (M: Cu/Ni and OIAC: ([2-oxo-1H-indol-3-ylidene]amino)chitosan) were electron beam irradiated by different doses (100 Gy, 1 kGy and 10 kGy). The electron beam has shown potential impact on biopolymer's support, in detail chain linking and chain scissoring, as evidenced by viscosity studies, FT-IR and X-ray diffraction spectroscopic techniques. Due to these structural changes, thermal properties of the complexes were found to be changed.

Antimicrobial activity and stability of electron beam irradiated dental irrigants.

Background: The electron beam (e-beam) radiation is considered as an effective means of sterilization of healthcare products as well as to induce the structural changes in the pharmaceutical agents/drug molecules. In addition to structural changes of pharmaceutical it also induces the formation of low molecular weight compounds with altered microbiological, physicochemical and toxicological properties. Among the several known medicaments, sodium hypochlorite (NaOCl) and chlorhexidine digluconate (CHX) are used as irrigants in dentistry to kill the pathogenic microorganisms like Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans and Candida albicans inhabiting the oral cavity.

Influence of electron beam irradiation on spectral, thermal, morphological and catalytic properties of Co(II) complex immobilized on chitosan's Schiff base.

This study was carried out to investigate the effect of electron beam irradiation on the spectral and catalytic properties of chitosan supported (ONClCl) tetra coordinated Co(II) complex, [Co(OIAC)Cl2]. The complex was subjected to electron beam irradiation of 100 Gy, 1 kGy and 10 kGy doses. Chain scission of chitosan was observed on irradiation at 100 Gy and 10 kGy and chain linking at 1 kGy as evidenced by viscosity and FT-IR spectroscopic studies.

The efficacy of nardostachys jatamansi against the radiation induced haematological damage in rats.

Introduction: Radiation is increasingly being used for medical purposes and it is an established weapon in the diagnosis and the therapy of cancer. An exposure to 1-2 Gys causes the NVD (Nausea, vomiting, diarrhoea) syndrome, whereas an exposure to 2-6 Gys causes the haematopoietic syndrome. The aim of the present study was to investigate the protective effect of the Nardostachys jatamansi root extract (NJE) on the radiation induced haematological damage in rats.

Photo-bio-synthesis of irregular shaped functionalized gold nanoparticles using edible mushroom Pleurotus florida and its anticancer evaluation.

A green chemistry approach to the synthesis of gold nanoparticles using edible mushroom Pleurotus florida (Oyster mushroom) by photo-irradiation method has been attempted. The mixture containing the aqueous gold ions and the mushroom extract was exposed to sunlight; this resulted in the formation of biofunctionalized gold nanoparticles. These nanoparticles were characterized using various techniques like UV-visible spectroscopy; X-ray diffraction studies, Energy dispersive X-ray analysis, Field emission scanning electron microscopy, Atomic force microscopy, Transmission electron microscopy and Fourier transform infrared spectrometry.

Dose rate effect on micronuclei induction in human blood lymphocytes exposed to single pulse and multiple pulses of electrons.

The effects of single pulses and multiple pulses of 7 MV electrons on micronuclei (MN) induction in cytokinesis-blocked human peripheral blood lymphocytes (PBLs) were investigated over a wide range of dose rates per pulse (instantaneous dose rate). PBLs were exposed to graded doses of 2, 3, 4, 6, and 8 Gy of single electron pulses of varying pulse widths at different dose rates per pulse, ranging from 1 × 10(6) Gy s(-1) to 3.2 × 10(8) Gy s(-1).

Dose rate effect of pulsed electron beam on micronucleus frequency in human peripheral blood lymphocytes.

The micronucleus assay in human peripheral blood lymphocytes is a sensitive indicator of radiation damage and could serve as a biological dosimeter in evaluating suspected overexposure to ionising radiation. Micronucleus (MN) frequency as a measure of chromosomal damage has also extensively been employed to quantify the effects of radiation dose rate on biological systems. Here we studied the effects of 8 MeV pulsed electron beam emitted by Microtron electron accelerator on MN induction at dose rates between 35 Gy min-1 and 352.

The effect of electron and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human blood lymphocytes.

The effect of electrons and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human peripheral blood lymphocytes was investigated to understand the relative biological effectiveness (RBE) of electrons compared with gamma rays. Blood samples were irradiated with an 8 MeV pulsed electron beam, at a mean instantaneous dose rate of 2.6 x 10(5) Gy s(-1).