A Novel Highly Sensitive Derivatization-Free Spectrofluorimetric Approach for Analysis of the Antifungal Terconazole in Dosage Forms, Biological Fluids, and Environmental Water Samples: Assessment of Greenness and Blueness Profiles.

In the current study, the triazole antifungal terconazole (TRZ) was determined using a novel, extremely sensitive, and environmentally friendly spectrofluorimetric approach. A methanolic solution of TRZ exhibited strong native fluorescence at 373 nm when excited at 241 nm. With a correlation coefficient of 0.

Deciphering the binding mechanism of tafamidis to calf thymus DNA: multimodal spectroscopic, thermodynamic, and computational perspectives.

The interaction between small molecules and biological macromolecules is a crucial area of research with significant implications across various scientific disciplines. Tafamidis is a clinically approved drug for transthyretin-mediated amyloidosis, yet its molecular interactions with biological macromolecules such as DNA remain unexplored. Investigating such interactions is crucial for understanding its broader pharmacodynamic profile and potential off-target effects.

Unraveling the molecular interaction of Larotrectinib with calf thymus DNA: A comprehensive study using multi-spectroscopic, thermodynamic, and computational techniques.

The study of the interaction between small molecules and biological macromolecules is a critical area of research with significant implications across various scientific fields. Larotrectinib, a tropomyosin kinase inhibitor, is used to treat patients with solid tumors harboring neurotrophic tyrosine receptor kinase (NTRK) gene fusions. In this investigation, the interaction between larotrectinib and calf thymus DNA (ctDNA) was thoroughly examined using a combination of techniques, including UV-Vis spectrophotometry, spectrofluorimetry, viscosity measurements, ionic strength variation, thermodynamic analysis, molecular dynamics simulations, and docking studies.

D-optimal candexch algorithm-enhanced machine learning UV-spectrophotometry for five-analyte determination in novel anti-glaucoma formulations and ocular fluids: four-color sustainability framework with NQS assessment and UN-SDG integration.

The novel anti-glaucoma ophthalmic preparation containing latanoprost, netarsudil, and benzalkonium chloride has posed a significant challenge due to its complexity and the lack of environmentally sustainable quantification methods, with only a single published method available for its quantification that lacks environmental consideration. This study aims to address this crucial gap by presenting a novel and sustainable approach using machine learning-enhanced UV-spectrophotometric chemometric models for the concurrent quantification of latanoprost, netarsudil, benzalkonium chloride, and two related compounds in ophthalmic preparations and aqueous humour. A strategic multi-level, multi-factor experimental design creates a 25-mixture calibration set for four models (PLS, GA-PLS, PCR, and MCR-ALS).

Eco-friendly QbD-optimized chromatographic method for simultaneous analysis of metronidazole and nicotinamide with applications in permeation, stability, and sustainability evaluation.

Metronidazole (MTZ) and nicotinamide (NCT) are co-formulated in Anaero® gel to treat skin conditions like acne and rosacea through combined antimicrobial and anti-inflammatory effects. This study presents the first validated in vitro permeation test (IVPT) method using Franz cells to assess the gel's drug release profile. Chromatographic separations were conducted with an RP-HPLC-photodiode array (PDA) system equipped with an ODS column using gradient elution of ethanol and phosphate buffer (10 mM, pH 3.

Jute Mallow Leaves Biomass as a Green Source for the Microwave-Assisted Synthesis of N,S-Carbon Quantum Dots: Application to the Sensitive Sensing of the Anticancer Drug Afatinib in Environmental Water Samples.

This research presents a novel and efficient microwave-assisted method for synthesizing nitrogen- and sulfur-doped carbon quantum dots (N,S-CQDs) using Corchorus olitorius leaves (Jute mallow) as a sustainable green carbon source and thiourea as a nitrogen and sulfur source. The method achieves remarkable efficiency, enabling the synthesis of N,S-CQDs in just 50 s. Diverse characterization approaches validated the distinctive optical and morphological characteristics of the N,S-CQDs.

Genetic polymorphisms of ABCC2 and CBR3 can influence the efficacy and toxicity of doxorubicin therapy in Egyptian patients with non-Hodgkin lymphoma.

This study investigated the impact of single nucleotide polymorphisms (SNPs) in genes involved in doxorubicin (DOX) transport and metabolism on clinical outcomes and toxicity in Egyptian patients with non-Hodgkin lymphoma. Ninety-two patients received at least six DOX treatment cycles. SNP genotyping was performed using real-time PCR with high-resolution melting analysis.

A novel ultrasensitive derivatization-free synchronous fluorescence approach for the simultaneous analysis of propofol and nalbuphine in human plasma and dosage forms: Compliance with greenness and blueness metrics.

This study presents a very sensitive and eco-friendly synchronous spectrofluorimetric method for the simultaneous quantification of propofol (PRP) and nalbuphine (NAL) for the first time. The technique used the intrinsic fluorescence characteristics of the two drugs, providing enhanced sensitivity and specificity. The two drugs were assessed simultaneously at 217 nm and 281 nm for PRP and NAL, respectively, with a synchronous wavelength difference (Δλ) of 80 nm.

Targeted synthesis of a trimethoxyphenyltetrahydropyrimidine analogue designed as a DNA intercalator: , multi-spectroscopic, thermodynamic, and approaches.

Based on the rational design of DNA intercalators and Topo-II inhibitors and taking into consideration the main pharmacophoric features of doxorubicin (Dox) as a reference standard, we theoretically designed novel substituted tetrahydropyrimidine analogues (T). The designed analogues (T) were investigated for their inhibitory potential towards the hybrid DNA and Topo-II target receptor using molecular docking. Interestingly, the theoretically designed analogue T with a 3,4,5-trimethoxy phenyl side chain was found to be the superior candidate, achieving a binding score of -7.

Ultrafast green synthesis of silver nanoparticles as fluorescent nanosensors for determination of isoniazid and nitrofurantoin in biological fluids and pharmaceuticals.

The current study presents a green, straightforward, and ultrafast microwave-assisted method for the synthesis of Ag-NPs in only 40 s, using the Paeonia officinalis root extract as a reducing agent, for the first time. Characterization of the produced Ag-NPs was carried out using different spectroscopic and microscopic methods. They exhibited strong emission peak at 318 nm following excitation at 227 nm.

Unlocking the power of nanohybrids: A critical review on carbon nanomaterial-functionalized silver nanoparticles for advanced antimicrobial applications.

Over the last decades, nanotechnology has enabled the development of several inorganic nanoparticles with significant biocidal properties against diverse microorganisms. Silver nanoparticles (AgNPs) are among the most promising antimicrobial nanomaterials that have attracted substantial attention in various fields due to their low cost, low toxicity, biocompatibility, photo and chemical stability, easy preparation, high fluorescence, and tunability. Carbon nanomaterials (CNMs) are another appealing nanomaterial with antimicrobial qualities.

Rapid Microwave-Assisted Synthesis of Nitrogen-Doped Carbon Quantum Dots as Fluorescent Nanoprobe for Sensitive Estimation of Tizanidine in Human Urine and Dosage Forms: Application to Content Uniformity Testing.

This study introduces a novel sensitive and environment-friendly spectrofluorimetric method for determination of an important muscle relaxant drug, tizanidine, with no need for using organic solvents or any pre-derivatization steps. This strategy depends on tizanidine quenching effect on the native fluorescence of newly synthesized nitrogen-doped carbon quantum dots (N-CQDs) from Tetraclinis articulata extract, for the first time, using a microwave-assisted synthetic strategy in only 4 min. Full characterization of the synthesized N-CQDs was performed by different microscopic and spectroscopic techniques.

Ultrasensitive analysis of the commonly abused CNS antitussive, dextromethorphan in biological fluids and dosage forms using a novel micellar-sensitized spectrofluorimetric approach: Compliance with greenness and blueness metrics.

The current study introduces the first micellar-enhanced spectrofluorimetric approach for the estimation of the commonly abused CNS antitussive, dextromethorphan (DXM) in its syrup and biological fluids. A micellar solution of sodium dodecyl sulfate (SDS) containing DXM showed high native fluorescence emission at 305 nm following excitation at 224 nm. Using SDS as a micellar system resulted in about a 2.

Leveraging Green Chromatography in Quality-by-Design-Driven Study for the Simultaneous Analysis of Essential Single-Pill Antidiabetic Drug Combinations.

Micellar liquid chromatography (MLC) has proven beneficial efficiency and ecological impact for routine quality control activities. In the proposed study, cyrene was investigated for the first time, together with other green additives, as a novel safe organic solvent in reversed-phase MLC. Quality-by-design (QbD) approach screened their effect on the separation performance.

A Novel Ultrasensitive Second Derivative Synchronous Spectrofluorimetric Approach for the Simultaneous Analysis of the Co-Administered Antihypertensives, Telmisartan and Carvedilol in Biological Fluids and Tablets: Greenness and Blueness Assessment.

Hypertension is the predominant risk factor for cardiovascular diseases and mortality. This study presents the first method for the simultaneous analysis of the co-administered antihypertensive drugs, Carvedilol (CAR) and Telmisartan (TEL) using a fast, highly sensitive, environmentally friendly, and cost-effective second derivative synchronous spectrofluorimetric approach. The fluorescence of CAR and TEL was quantified at 243 nm and 274.

HPLC-PDA and in vivo anti-inflammatory potential of isorhamnetin-3-O-β-D-glucoside from Zygophyllum simplex L.

Ethnopharmacological Relevance: Inflammation is a biological process in response to injury, resulting in altered blood flow, increased vascular permeability, tissue destruction, and the production of reactive oxygen species (ROS) and inflammatory mediators. Zygophyllum simplex L., a medicinal plant traditionally used in the Arabian Peninsula for inflammatory disorders, has demonstrated promising in vitro anti-inflammatory activity due to its phenolic content.

Eco-friendly four spectrophotometric approaches for the simultaneous determination of the recently FDA-approved combination, bupivacaine and meloxicam in pharmaceutical dosage forms.

In this study, bupivacaine (BUP) and meloxicam (MLX) were simultaneously assayed in their co-formulated ampoules without interference using four affordable, sensitive, and eco-friendly spectrophotometric methods. The assay of MLX at 359.3 nm over the concentration range of 1.

HPLC-PDA Analysis of Polyacetylene Glucosides from and Their Antibacterial and Antibiofilm Properties against .

: Bacterial resistance and virulence are challenges in treating bacterial infections, especially in . Plants of the Cass. genus are used traditionally to address a variety of diseases, including infections, but the potential bioactive compounds are unknown.

Design and synthesis of high quantum yield doped carbon nano probe derived from household sources for sensing of the anti-GERD drug pantoprazole.

This contribution aims to design and validate a new green, cheap, and fast approach for determining the anti-GERD drug pantoprazole in different matrices. New S and N-doped carbon nanomaterials (S,N-CNMs) have been prepared via microwave irradiation of a mixture of widely available household sources. Remarkably, the utilization of a blend of carbamide and thiocarbamide with table sugar yields S,N-CNMs exhibiting the utmost quantum yield (54 %), hydrophilicity, as well as stable, homogeneous, and diminutive particle size distribution.

A highly sensitive first derivative synchronous spectrofluorimetric approach for the simultaneous analysis of the anti-breast cancer co-administered drugs, letrozole and tramadol in dosage forms and human plasma at nanogram levels.

Letrozole is an anticancer medication prescribed for the management of estrogen receptor-positive breast cancer in postmenopausal women. Chronic pain is prevalent in patients receiving chemotherapy, leading to the use of adjuvant analgesics such as tramadol. This work introduces the first analytical approach for the concurrent quantification of letrozole and tramadol, two co-administered drugs, employing a rapid, highly sensitive, eco-friendly, and cost-effective first derivative synchronous spectrofluorimetric technique.

A sustainable sensitive spectrofluorimetric approach for the determination of the multipotent protein lactoferrin in different pharmaceuticals and infant milk formula: Compliance with greenness metrics.

The current study presents the first spectrofluorimetric approach for the estimation of lactoferrin, depending on the measurement of its native fluorescence at 337 nm after excitation at 230 nm, without the need for any hazardous chemicals or reagents. It was found that the fluorescence intensity versus concentration calibration plot was linear over the concentration range of 0.1-10.

Novel fluorescent probes based on sulfur and nitrogen co-doped carbon dots for determination of three N-substituted phenothiazine derivatives in dosage forms.

In the current work, sulfur and nitrogen co-doped carbon dots (S,N-CDs) as simple, sensitive, and selective turn-off fluorescent nanosensors were utilized for analysis of three phenothiazine derivatives, including acetophenazine (APZ), chlorpromazine (CPH), and promethazine (PZH). S,N-CDs were synthesized through a green one-pot microwave-assisted technique using widely available precursors (thiourea and ascorbic acid). HRTEM, EDX, FTIR spectroscopy, UV-Vis absorption spectroscopy, and fluorescence spectroscopy were used to characterize the as-synthesized CDs.

The effects of genetic polymorphism on toxicity and pharmacokinetics of methotrexate in Egyptian adult patients with leukaemia or lymphoma.

Polymorphisms in genes coding folate-metabolising enzymes might alter the pharmacokinetics and sensitivity for methotrexate "MTX".The aim of the study aimed to investigate the influence of MTHFR C677T, DHFR19 Ins/del, GGH -401 C > T, and MTR A2756G polymorphisms on MTX toxicity and pharmacokinetics in Egyptian patients with Acute lymphoblastic leukaemia (ALL) or Non-Hodgkin lymphoma (NHL).Fifty adult Egyptian patients with ALL and NHL, treated with high dose MTX, were prospectively enrolled in the study.

Formulation of Deformable Liponiosomal Hybrid of Repaglinide: In vitro Characterization and Evaluation of the Anti-Diabetic Effect.

Purpose: The current study sought to create novel deformable liponiosomal hybrids (LNHs) as a viable RPG delivery system. Repaglinide (RPG) is an effective anti-hyperglycemic drug. However, its limited solubility may limit its therapeutic applicability.