Binding interactions between ar-turmerone and human serum albumin: mechanistic and biophysical insights.

Ar-turmerone, a major bioactive compound in , has been reported to exhibit anti-inflammatory, antiproliferative, antifungal, antioxidant, and anticancer properties. This study investigated its interaction with human serum albumin (HSA) using spectroscopic, microscopic, and computational approaches to characterize its binding properties, which are crucial for its distribution and transport in the bloodstream. Fluorescence spectroscopy showed a 34% decrease in HSA fluorescence intensity at 90 μM ar-turmerone, accompanied by a 2 nm blue shift, suggesting a transition of the protein's fluorophores' microenvironment to a slightly nonpolar state.

Integrative analysis of the anti-diabetic inhibitor GW9508 binding to human serum albumin: computational, spectroscopic and ITC insights.

GW9508 (GW) is a promising anti-diabetic agent with potential to regulate glucose metabolism. This study explored its binding interaction with human serum albumin (HSA), the major blood transport protein, using modeling, isothermal titration calorimetry (ITC), and various spectroscopic techniques. Molecular docking suggested that GW preferentially binds to subdomain IIA (Site I) of HSA, stabilized by hydrogen bonding, hydrophobic interactions, and van der Waals forces.

Synthesis of new nicotintaldehyde derivatives via Pd(0) catalyzed suzuki coupling with structural characterization and co-combined computational-experimental evaluation against oral pathogens.

A highly efficient Pd-built catalytic system for the Suzuki Miyaura coupling of nicotinaldehyde was developed under gentle reaction conditions. Analytical techniques included FTIR, Uv-Visible, H NMR, and C NMR, whereas computational analysis used during the investigation involved DFT (density functional theory), Molecular docking, PASS, ADMET and drug likeness. In the current investigation, new nicotinaldehyde derivatives were synthesized via a Pd(0)-catalyzed Suzuki coupling reaction.

Characterization of dovitinib-human serum albumin association potential through optical spectroscopic and in silico approaches.

The multitargeted receptor tyrosine kinase inhibitor, dovitinib (DTB) is reported to have a broad variety of pharmacological properties, which can be potentially useful in the treatment of various cancer entities. Association of DTB with the carrier protein, human serum albumin (HSA) was explored via experimental and in silico techniques. The reduction results of HSA fluorescence along with the hyperchromic effect in HSA's absorption signal observed with added DTB affirmed the DTB-HSA complex formation.

Immediate effects of muscle energy technique on pain and range of motion in patients with chronic non-specific neck pain: A randomized controlled trial.

Objective: To determine the immediate effects of muscle energy technique and conventional physical therapy compared to conventional physical therapy alone on pain and range of motion (ROM) in patients with chronic mechanical neck pain.

Methods: A randomized controlled trial (RCT) was conducted at Physical Therapy Department Hayatabad Medical Complex Peshawar from March 2022 to August 2022. A total of 50 subjects, aged 18 to 50 years with non-specific neck pain for ≥ 3 months were included.

Investigation of the interaction between bromocriptine and human serum albumin with spectroscopic techniques and computational approaches.

Fluorescence and absorption spectroscopy coupled with molecular docking techniques were chosen to reveal the molecular relationship between bromocriptine (BRC), a dopamine agonist, and human serum albumin (HSA), the major carrier protein in the bloodstream. The gradual decrease of Stern-Volmer constant (K) with increasing temperatures noted that BRC induced quenching in HSA fluorescence occurred a static quenching mechanism, thus indicating the formation of complexes between BRC and HSA. The changes observed in the UV-vis absorption spectra of HSA as a result of BRC addition and the fact that the biomolecular quenching rate constants (K) calculated for the BRC-HSA interaction are significantly larger than the highest value of the dynamic quenching rate constant also support this conclusion.

Mitigating maize stalk rot disease: harnessing Bacillus subtilis PM57 and Bacillus cereus PM38 as biocontrol allies against Erwiniacarotovora.

Maize bacterial stalk rot, caused by Erwinia carotovora pv. zeae (EC) is a significant threat to maize production, and applicable biocontrol measures are lacking. This study aims to identify a potent biocontrol agent against Erwinia carotovora and enhance plant growth under stress conditions.

Probing the articaine-human serum albumin interaction and influences of paracetamol and caffeine on the interaction by spectroscopy, voltammetry, and bioinformatics.

Interaction between a local anesthetic drug, articaine (ART) and human serum albumin (HSA) was investigated in the absence and presence of paracetamol (PAR) and caffeine (CAF) using spectroscopic, voltammetric, and computational techniques for the first time. The results demonstrated that increasing concentrations of ART in HSA solution led to a decrease in HSA fluorescence signal, indicating the ART-HSA complex formation via the static quenching mechanism. The binding strength of the complex was moderate (binding constant, K = 5.

Genome-Wide Identification and Expression Profile of () Gene Family in L.

The biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are essential for sesquiterpenes and triterpenes, respectively, is primarily governed by the mevalonate pathway, wherein () plays a pivotal role. This study identified eight members of the FPS gene family in , designated -, through bioinformatics analysis, revealing their distribution across several chromosomes and a notable tandem gene cluster. The genes exhibited strong hydrophilic properties and key functional motifs crucial for enzyme activity.

Impact of a Medication Reconciliation Improvement Package on Adherence to Medication Reconciliation Among Internal Medicine Physicians: A Quality Improvement Project in a Lower-Middle Income Country.

Medication reconciliation is one of the best measures to prevent medication-related errors at the time of admission and discharge of patients. We conducted a quasi-experimental study to evaluate the impact of a Medication reconciliation improvement package (intervention) on adherence to medication reconciliation at the time of admission in Department of Internal Medicine. The study included all adult patients admitted to internal medicine from August 2019 to December 2020.

An ultra-dense linkage map identified quantitative trait loci corresponding to fruit quality- and size-related traits in red goji berry.

Goji berries are a small-fruited shrub with industrial importance whose fruit considered beneficial in both fresh and dried forms. Current germplasms of goji berries include small fruits with a short shelf life, less sweet and bitter taste, and a lack of appropriate genetic information. This study aimed to employ whole genome resequencing to generate an ultra-dense bin linkage map and to elucidate the genetic basis of goji fruit quality and size using quantitative trait loci (QTL) mapping analysis in a cross-pollinated hybrid population.

Performance analysis of high voltage disc insulators with different profiles in clean and polluted environments using flashover, withstand voltage tests and finite element analysis.

Severe pollution-induced flashovers on insulators present a pressing challenge to power system safety. The frequent failure of high-voltage insulators, particularly in the polluted environments of Pakistan, poses a critical concern. This paper investigates the impact of insulator profile on reducing pollution flashovers, testing two designs as per IEC standard 60383 and simulated using the Finite Element Method in COMSOL Multiphysics®.

Geographical targeting of active case finding for tuberculosis in Pakistan using hotspots identified by artificial intelligence software (SPOT-TB): study protocol for a pragmatic stepped wedge cluster randomised control trial.

Introduction: Pakistan has significantly strengthened its capacity for active case finding (ACF) for tuberculosis (TB) that is being implemented at scale in the country. However, yields of ACF have been lower than expected, raising concerns on its effectiveness in the programmatic setting. Distribution of TB in communities is likely to be spatially heterogeneous and targeting of ACF in areas with higher TB prevalence may help improve yields.

Characterization, anti-microbial, anti-oxidant and growth promoting effects of biogenically synthesized silver nanoparticles derived from Dicliptera bupleuroides Nees.

One of the most important areas of nanotechnology is the use of nanoparticles (NPs) for a variety of environmental and biological applications, with silver nanoparticles (Ag-NPs) gaining a lot attention due to their distinct properties. The current study deals with the synthesis of Ag-NPs from Dicliptera bupleuroides Nees leaf extract and to determine their antioxidant, antimicrobial potential and effects on wheat seed germination and growth. UV-Visible spectrum revealed a prominent absorption peak at 442 nm, elucidating the conformation of the Ag-NPs synthesis.

Microbial detoxification of chlorpyrifos, profenofos, monocrotophos, and dimethoate by a multifaceted rhizospheric Bacillus cereus strain PM38 and its potential for the growth promotion in cotton.

Bacillus genera, especially among rhizobacteria, are known for their ability to promote plant growth and their effectiveness in alleviating several stress conditions. This study aimed to utilize indigenous Bacillus cereus PM38 to degrade four organophosphate pesticides (OPs) such as chlorpyrifos (CP), profenofos (PF), monocrotophos (MCP), and dimethoate (DMT) to mitigate the adverse effects of these pesticides on cotton crop growth. Strain PM38 exhibited distinct characteristics that set it apart from other Bacillus species.

Deciphering the mechanistic role of Bacillus paramycoides (PM51) and Bacillus tequilensis (PM52) in bio-sorption and phyto-assimilation of Cadmium via Linum usitatissimum L. Seedlings.

Three Cd resistant bacterium's minimal inhibition concentrations were assessed and their percentages of Cd accumulation were determined by measurements using an atomic absorption spectrophotometer (AAS). The results revealed that two isolates Bacillus paramycoides (PM51) and Bacillus tequilensis (PM52), identified by 16S rDNA gene sequencing, showed a higher percentage of Cd accumulation i.e.

Hemorrhagic Ligamentum Flavum Cyst in the Adjacent Level of Lytic Spondylolisthesis - A Case of Diagnostic and Therapeutic Dilemma.

Introduction: Occurrence of hemorrhagic cyst inside ligamentum flavum is a very rare phenomenon and presents with back pain, radiculopathy, or neurogenic claudication. Various causes reported in the literature are trauma, anticoagulant therapy, and increased micromotion in the setting of unstable and degenerated motion segment.

Case Report: We report a case of 41-year-old male patient who presented with claudication pain in both lower limbs for the past 6 months associated with bilateral calf atrophy.

Multi-gene panel sequencing in highly consanguineous families and patients with congenital forms of skeletal dysplasias.

Skeletal dysplasias (SKDs) are a heterogeneous group of more than 750 genetic disorders characterized by abnormal development, growth, and maintenance of bones or cartilage in the human skeleton. SKDs are often caused by variants in early patterning genes and in many cases part of multiple malformation syndromes and occur in combination with non-skeletal phenotypes. The aim of this study was to investigate the underlying genetic cause of congenital SKDs in highly consanguineous Pakistani families, as well as in sporadic and familial SKD cases from India using multigene panel sequencing analysis.

Application of carboxymethylcellulose in combination with essential oils nano-emulsions edible coating for the preservation of kiwifruit.

The present research investigates the effectiveness of nano-emulsified coatings (C-1, C-2, and C-3) in preserving the kiwifruit at a temperature of 10 ± 2 °C with 90-95 % relative humidity (RH) for 30 days. The nano-emulsions were prepared from varied carboxymethyl cellulose (CMC) concentrations with different combinations of essential oils such as thyme, clove, and cardamom. Dynamic light scattering investigation with Zeta Sizer revealed that C-1, C-2, and C-3 nano-emulsions have nano sizes of 81.

The Problem of Occult Pyelonephritis: Acute Pyelonephritis in Patients Presenting Only with Lower Urinary Tract Symptoms.

Pyelonephritis is a prevalent diagnosis of emergency department visits. It commonly presents as flank pain and costovertebral tenderness with urinary symptoms. However, some cases occur without typical symptoms.

5-Fluorouracil-Loaded Hyaluronic Acid-Coated Niosomal Vesicles: Fabrication and Ex Vivo Evaluation for Skin Drug Delivery.

5-Fluorouracil (5-FU) is one of the most potent drugs against solid tumors. However, its parenteral administration is associated with systemic toxicity, while its topical application has limited percutaneous absorption. To overcome these limitations, the current study undertakes the formulation of 5-FU as niosomal vesicles that were coated with hyaluronic acid to improve its targeting efficiency for cancer cells.

Deciphering the binding mode and structural perturbations in floxuridine-human serum albumin complexation with spectroscopic, microscopic, and computational techniques.

The binding mode of antineoplastic antimetabolite, floxuridine (FUDR), with human serum albumin (HSA), the leading carrier in blood circulation, was ascertained using multi-spectroscopic, microscopic, and computational techniques. A static fluorescence quenching was established due to decreased K values with rising temperatures, suggesting FUDR-HSA complexation. UV-vis absorption spectral results also supported this conclusion.