Two new polyacetylenes from the roots of .

In this study, two new polyacetylenes were isolated from the extract portion with ethyl acetate from roots by implementing various chromatography techniques, such as the use of silica gel under normal-phase conditions, MPLC, molecular sieving utilising Sephadex LH-20, and purification through semi preparative HPLC. Their structures were elucidated as ()-[()-deca-8-en-4,6-diynyl]-3-(4-hydroxy-3-methoxyphenyl) acrylate () and 6 -(4, 12)-tetradeca-4,12-dien-8,10-diyne-1,6-diyldiacetate () by multiple spectroscopic techniques such as 1D and 2D NMR, UV, IR, and HR ESI MS.

[Two new sesquiterpenoids from Aucklandiae Radix and their farnesoid X receptor agonist activity].

Various chromatographic methods were comprehensively applied to study the chemical composition of the ethyl acetate extract from Aucklandiae Radix. The structures of all compounds were identified by analyzing their physicochemical properties and using spectroscopic methods. Two new sesquiterpenoids, named auclappsines A and B(1 and 2) were isolated and identified.

Two new polyacetylenes from the roots of .

Two new polyacetylenes, (5,12)-14-hydroxytetradeca-5,12-dien-8,10-diyn-1-yl 3-methylbut-2-enoate () and 5, 1'-(6,12)-1-hydroxytetradeca-6,12-dien-8,10-diyn-5-yl 2-methylbutanoate (), together with two known ones, (2,8)-12--methylbutyryltetradeca-2,8-diene-4,6-diyne-1,14-diol (), and ()-5-[5-(but-3-en-1-yn-1-yl)thiophen-2-yl]pent-2-en-4-yn-1-yl acetate () were isolated from the ethyl acetate extract of the roots of by various chromatographic methods, such as normal phase silica gel column, MPLC, and semi-preparative HPLC. Their structures were identified by kinds of spectroscopic methods including 1D NMR, 2D NMR, IR, UV, HR-ESI-MS, and ECD. Compound exhibited cytotoxic activities in HepG2 and Huh7 cells with IC values of 20.

Chemical constituents from the roots of .

A new benzofuran-type neolignan (), two new phenylpropanoids ( - ), and one new C steroid () were isolated from the ethyl acetate extract of the roots of by chromatographic methods, including silica gel, ODS column chromatography, MPLC, and semi-preparative HPLC. Their structures were identified as dolosougenin A (), ()-3-isopropylpentyl ()-3-(4-hydroxy-3-methoxyphenyl) acrylate (), ()-3-isopropylpentyl ()-3-(4-hydroxy-3-methoxyphenyl) acrylate () and dolosoucin A () through various spectroscopic techniques including 1D NMR, 2D NMR, IR, UV, HR ESI MS, ORD, and computational ORD methods.

Ni-catalyzed arylation of alkynes with organoboronic acids and aldehydes to access stereodefined allylic alcohols.

A new, efficient and practical method for the three-component arylative coupling of aldehydes, alkynes and arylboronic acids has been developed through nickel catalysis. This transformation provides diverse -selective tetrasubstituted allylic alcohols without the use of any aggressive oragnometallic nucleophiles or reductants. Moreover, benzylalcohols are viable coupling partners oxidation state manipulation and arylative coupling in one single catalytic cycle.

Regio- and chemoselective hydroamination of unactivated alkenes with anthranils NiH-catalysis.

A NiH-catalyzed polarity-reversed hydroamination of β,γ-, γ,δ- and δ,ε-unsaturated alkenes with electrophilic anthranils was developed. This reaction proceeds in a highly regio- and chemoselective manner to afford γ, δ and ε-arylamines bearing a carbonyl or alcohol functionality with 100% atom efficiency. Preliminary mechanistic studies indicate that the chemoselectivity is controlled by the base and the alcohol product is derived from the base-catalyzed hydrosilylation of the CO bond.

Activation of Cannabinoid Receptor 1 in GABAergic Neurons in the Rostral Anterior Insular Cortex Contributes to the Analgesia Following Common Peroneal Nerve Ligation.

The rostral agranular insular cortex (RAIC) has been associated with pain modulation. Although the endogenous cannabinoid system (eCB) has been shown to regulate chronic pain, the roles of eCBs in the RAIC remain elusive under the neuropathic pain state. Neuropathic pain was induced in C57BL/6 mice by common peroneal nerve (CPN) ligation.

[Two new triterpeneoids from roots of Dolomiaea souliei].

The present study analyzed the chemical constituents in dried roots of Dolomiaea souliei. Chromatographic methods, such as normal-phase, and reversed-phase column chromatography, TLC, and preparative HPLC, were applied to separate and purify the petroleum ether extract of D. souliei.

Schisanwilsonins H and I, two new dibenzocyclooctane lignans from the fruits of .

Two new dibenzocyclooctane lignans, schisanwilsonins H () and I (), together with eight known compounds gomisin J (), wulignan A (), gomisin S (), tigloylgomisin P (), gomisin O (), (-)-gomisin K (), rubschisantherin () and wuweizisu C () were isolated from the 95% ethanol extract of the fruits of . exhibited anti-HBV activity with potency against HBsAg and HBeAg secretion by 37.1% and 32.

Regular Aerobic Exercise Attenuates Pain and Anxiety in Mice by Restoring Serotonin-Modulated Synaptic Plasticity in the Anterior Cingulate Cortex.

Purpose: Clinical studies found that regular aerobic exercise has analgesic and antianxiety effects; however, the underlying neural mechanisms remain unclear. Multiple studies have suggested that regular aerobic exercise may exert brain-protective effects by promoting the release of serotonin, which may be a pain modulator. Anterior cingulate cortex (ACC) is a key brain area for pain information processing, receiving dense serotonergic innervation.

Lignans from the seeds of Herpetospermum pedunculosum and their farnesoid X receptor-activating effect.

The seeds of Herpetospermum pedunculosum (Ser.) C.B.

[A novel alkaloid from Corydalis tomentella].

The chemical constituents in the ethyl acetate extract of Corydalis tomentella was isolated and purified with normal and reversed phase silica gel column chromatography, Sephadex LH-20, MCI, and semi-preparative HPLC. The compound structures were identified based on spectroscopic experiments and reported papers. Finally, eighteen compounds(1-18) were obtained from C.

Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism.

Background: Cholestasis is characterized by accumulation of bile components in liver and systemic circulation. Restoration of bile acid homeostasis via activating farnesoid x receptor (FXR) is a promising strategy for the treatment of cholestasis. FXR-SHP (small heterodimer partner) axis plays an important role in maintaining bile acid homeostasis.

Ptehoosines A and B: Two new sesamin-type sesquilignans with antiangiogenic activity from Pterocephalus hookeri (C.B. Clarke) Höeck.

Two undescribed sesamin-type sesquilignans ptehoosines A (1) and B (2), together with 4 known lignans (3-6), were isolated from Pterocephalus hookeri (C.B. Clarke) Höeck which was widely used as traditional Tibetan medicine for treatment of rheumatoid arthritis.

Dolominol a and B, two new neolignans from (Franch.) C.Shih.

Two new neolignans, dolominol A (1) and dolominol B (2), together with 12 known lignans, -(7,8)-guaiacyl-glycerol--O-4'-dihydroconiferyl ether (3), -(7,​8)-1-​(4-​hydroxy-​3-​methoxyphenyl)​-​2-{4-[()-​3-​hydroxy-​1-​propen​yl)​]-​2-​methoxyphenoxy}-1,​3-​propanediol (4), (-)-dihydrodehydrodiconiferyl alcohol (5), (-)-massoniresinol (6), vladinol D (7), syringaresinol (8), prinsepiol (9), medioresinol (10), (+)-pinoresinol (11), 2-guaicyl-4-oxo-6-catechyl-3,7-dioxabicyclo [3.3.0]octane (12), cycloolivil (13), isolariciresinol (14) were isolated from (Franch.

Musculoskeletal Pain during the Menopausal Transition: A Systematic Review and Meta-Analysis.

Musculoskeletal pain (MSP) is one of the most severe complaints in women undergoing menopause. The prevalence of MSP varied when taking the menopausal state and age factor into consideration. This study investigated the prevalence of MSP in perimenopausal women and its association with menopausal state.

Two new lignans from .

, its peels possessed better special flavour, as well as various bioactivities, such as anti-inflammatory, anti-microbial and anti-tumour. In our chemical investigation on the peels of , two new lignans ( and ) and three known lignans (-) were isolated by silica gel column chromatography, ODS column and preparative HPLC and their structures were established as zanthlignans A and B (), (-)-asarinin (), phylligenin () and planispine A () through various spectroscopic techniques including UV, IR, HR-ESI-MS, NMR and CD methods.

Berberisides A-D: three novel prenylated benzoic acid derivatives and a clerodane glycoside from aherndt.

Three undescribed prenylated benzoic acid derivatives berberisides A-C () and a new clerodane glycoside berberiside D () were isolated from Aherndt. Their structures were elucidated on the basis of extensive NMR and HR-ESI-MS analysis. The cytotoxic activities of all isolates were studied against lung carcinoma A549, hepatocellular carcinoma HepG2 and breast carcinoma MDA-MB-231 cell lines.

Cytotoxic triterpenoid saponins from .

Two new cycloartane glycosides, cycloatriosides A and B (), and a new oleanolic acid glycoside, thaliatrioside A (), along with known triterpenoids () were isolated from . The structures of the new compounds were established as 3---D-galactopyranosyl (20, 24 )-3,16,25,29-tetrahydroxy-20,24-epoxycycloartane-29-O--D-glucopyranoside (), 3---D-glucopyranosyl-(1→2)--arabinopyranosyl-3,22,30-trihydroxycycloart-24-en-21-oic acid -L-arabinopyranosyl-(1→6)--D-glucopyranoside () and 3--[-L-rhamnopyranosyl-(1→3)--D-xylopyranosyl-(1→3)--L-rhamnopyranosyl-(1→2)--L-arabinopyranosyl]-oleanolic acid 28---D-glucopyranosyl ester () on the basis of extensive NMR and HR-ESI-MS analyses, along with acid hydrolysis. Their cytotoxic activities against human lung cancer cells A549 and human breast cancer cells MDA-MB-231 were evaluated using MTT method.

Quantitative Characterization of Ginsenoside Biotransformation in Inflorescences and Leaves by Online Two-Dimensional Liquid Chromatography Coupled to Mass Spectrometry.

inflorescences (PNI) and leaves (PNL) are commonly used as folk medicine and food supplements. In this study, an online two-dimensional hydrophilic interaction × reversed-phase liquid chromatography coupled to linear trap quadropole mass spectrometry method was developed to determine 24 ginsenosides, including two novel compounds, in PNI and PNL extracted by water and methanol. Our data demonstrated that ginsenosides Rd, Rc, Rb2, Rb3, Rb1, Ra2, Ra1, and Ra3 in both PNI and PNL extracted by water rather than methanol can be transformed to ginsenoside F2, notoginsenoside Fe, ginsenoside Rd2, notoginsenoside Fd, gypenoside XVII, PN02, PN01, and PN03, respectively, by selectively cleaving the β-(1→2)-glucosidic linkage at the C-3 position.

Structural characterization and immunoregulatory activity of two polysaccharides from the rhizomes of Atractylodes lancea (Thunb.) DC.

A neutral polysaccharide (ALP-1) and an acidic polysaccharide (ALP-3) were purified from Atractylodes lancea (Thunb.) DC. ALP-1 exhibited a linear backbone composed of (2 → 1)-linked β-d-fructofuranose.

A new neolignan glycoside from .

One new neolignan glycoside, dolomiside A (), together with 11 known phenylpropanoid glycosides were isolated from (Franch.) Shih. The structures of these isolates were determined by UV, CD, HR-ESI-TOFMS, 1D and 2D NMR analysis.

Bacillamidins A⁻G from a Marine-Derived .

Seven long-chain amides, including five previously undescribed bacillamidins A⁻E (⁻) and two previously reported synthetic analogs, bacillamidins F () and G (), were isolated from extracts of the marine-derived strain RJA1515. The structures of the new compounds were established by extensive analysis of 1D and 2D nuclear magnetic resonance (NMR) data as well as high resolution mass spectrometry (HRMS), and the absolute configurations of the stereogenic carbons of ⁻ were established by comparison of the calculated and the experimental electronic circular dichroism (ECD) spectra. The cytotoxic and antimicrobial activities of ⁻ were evaluated.