A Layer-by-Layer Polycaprolactone/Chitosan-Based Biomimetic Hybrid Nanofibroporous Scaffold for Enhanced Skin Tissue Regeneration: Integrating Solution Blow Spinning and Freeze Casting Techniques.

Nanofibers, with their high surface area-to-volume ratio, elasticity, and mechanical strength, significantly enhance scaffold structures for skin tissue engineering. The present study introduces a unique method of combining solution blow spinning (SBS) and freeze casting to fabricate biomimetic hybrid nanofibroporous scaffolds (BHNS) using polycaprolactone (PCL) and chitosan (CH). The developed scaffolds mimic the fibrous porous natural extracellular matrix (ECM) architecture, promoting cell adhesion, proliferation, and matrix deposition.

Discovering hidden patterns: Association rules for cardiovascular diseases in type 2 diabetes mellitus.

Background: It is increasingly common to find patients affected by a combination of type 2 diabetes mellitus (T2DM) and coronary artery disease (CAD), and studies are able to correlate their relationships with available biological and clinical evidence. The aim of the current study was to apply association rule mining (ARM) to discover whether there are consistent patterns of clinical features relevant to these diseases. ARM leverages clinical and laboratory data to the meaningful patterns for diabetic CAD by harnessing the power help of data-driven algorithms to optimise the decision-making in patient care.

In vitro and in vivo assessment of curcumin-quercetin loaded multi-layered 3D-nanofibroporous matrix prepared by solution blow-spinning for full-thickness burn wound healing.

Burn wounds (BWs) cause impairment of native skin tissue and may cause significant microbial infections that demand immediate care. Curcumin (Cur) and quercetin (Que) exhibit antimicrobial, hemocompatibility, ROS-scavenging, and anti-inflammatory properties. However, its instability, water insolubility, and low biological fluid absorption render it challenging to sustain local Cur and Que doses at the wound site.

Biosensors integrated 3D organoid/organ-on-a-chip system: A real-time biomechanical, biophysical, and biochemical monitoring and characterization.

As a full-fidelity simulation of human cells, tissues, organs, and even systems at the microscopic scale, Organ-on-a-Chip (OOC) has significant ethical advantages and development potential compared to animal experiments. The need for the design of new drug high-throughput screening platforms and the mechanistic study of human tissues/organs under pathological conditions, the evolving advances in 3D cell biology and engineering, etc., have promoted the updating of technologies in this field, such as the iteration of chip materials and 3D printing, which in turn facilitate the connection of complex multi-organs-on-chips for simulation and the further development of technology-composite new drug high-throughput screening platforms.

Design strategies for composite matrix and multifunctional polymeric scaffolds with enhanced bioactivity for bone tissue engineering.

Over the past few decades, various bioactive material-based scaffolds were investigated and researchers across the globe are actively involved in establishing a potential state-of-the-art for bone tissue engineering applications, wherein several disciplines like clinical medicine, materials science, and biotechnology are involved. The present review article's main aim is to focus on repairing and restoring bone tissue defects by enhancing the bioactivity of fabricated bone tissue scaffolds and providing a suitable microenvironment for the bone cells to fasten the healing process. It deals with the various surface modification strategies and smart composite materials development that are involved in the treatment of bone tissue defects.

Target association rule mining to explore novel paediatric illness patterns in emergency settings.

Background And Aims: To assess the hospitalized sick children admitted to the pediatric emergency department (ED) and to find new patterns of clinical and laboratory attributes using association rule mining (ARM).

Methods: In this observational study, 158 children with median (IQR) age 11 months and a PRISM III score of 5 (2-9) were enrolled. Hotspot data mining method was applied to assess clinical attributes, lab investigations and pre-defined outcome parameters of children and their association in sick hospitalized children aged 1 month to 12 years.

Novel strategies for designing regenerative skin products for accelerated wound healing.

Healthy skin protects from pathogens, water loss, ultraviolet rays, and also maintains homeostasis conditions along with sensory perceptions in normal circumstances. Skin wound healing mechanism is a multi-phased biodynamic process that ultimately triggers intercellular and intracellular mechanisms. Failure to implement the normal and effective healing process may result in chronic injuries and aberrant scarring.

An image forensic technique based on JPEG ghosts.

The unprecedented growth in the easy availability of photo-editing tools has endangered the power of digital images. An image was supposed to be worth more than a thousand words, but now this can be said only if it can be authenticated or the integrity of the image can be proved to be intact. In this paper, we propose a digital image forensic technique for JPEG images.

Generation of graphene oxide and nano-bioglass based scaffold for bone tissue regeneration.

Graphene oxide (GO) offers a distinct opportunity in the field of biomedical engineering owing to its exceptionally high mechanical strength, excellent electrical conductivity, high optical transparency, and favorable biocompatibility. In this article, nanocomposite biocompatible GO-based scaffolds (chitosan/gelatin/nanobioglass/GO) Ch-G-NBG-GO were successfully fabricated through freeze drying technique (-40 °C) and evaluated for various physico-chemical and biological properties. The prepared Ch-G-NBG-GO composites have been investigated for their structural, physiochemical, and surface morphology via x-ray diffraction (XRD), high resolution scanning electron microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), energy-dispersive x-ray Spectroscopy and, differential scanning colorimetry (DSC) respectively.

Flavonoids as Promising Neuroprotectants and Their Therapeutic Potential against Alzheimer's Disease.

Alzheimer's disease (AD) is one of the serious and progressive neurodegenerative disorders in the elderly worldwide. Various genetic, environmental, and lifestyle factors are associated with its pathogenesis that affect neuronal cells to degenerate over the period of time. AD is characterized by cognitive dysfunctions, behavioural disability, and psychological impairments due to the accumulation of amyloid beta (A) peptides and neurofibrillary tangles (NFT).

Generation of hybrid tissue engineered construct through embedding autologous chondrocyte loaded platelet rich plasma/alginate based hydrogel in porous scaffold for cartilage regeneration.

Over the past decades, various attempts have been made to develop suitable tissue-engineered constructs to repair or regenerate the damaged or diseased articular cartilage. In the present study, we embedded Platelet rich plasma (PRP)/Sodium Alginate (SA) based hydrogel in porous 3D scaffold of chitosan (CH)/chondroitin sulfate (CS)/silk fibroin (SF) to develop hybrid scaffold for cartilage tissue construct generation with abilities to support shape recovery potential, facilitate uniform cells distribution and mimic gel like cartilage tissue extracellular matrix.The developed hybrid matrix shows suitable pore size (55-261 μm), porosity (77 ± 4.

Optimization and evaluation of ciprofloxacin-loaded collagen/chitosan scaffolds for skin tissue engineering.

A novel ciprofloxacin-loaded collagen-chitosan scaffold was developed for the treatment of wound using freeze drying method. The average pore size and porosity of developed scaffold was found to be around 125 µm and 91 ± 0.56%.

Design and evaluation of ciprofloxacin loaded collagen chitosan oxygenating scaffold for skin tissue engineering.

Hypoxia and sepsis are key concerns towards modern regenerative medicine. Oxygen generating biomaterials having antibacterial property aims to answer these concerns. Hypoxia promotes reactive oxygen species at the implant site that delays wound healing.

Ursolic acid attenuates oxidative stress in nigrostriatal tissue and improves neurobehavioral activity in MPTP-induced Parkinsonian mouse model.

Parkinson's disease (PD) is characterized by a slow and progressive degeneration of dopaminergic neurons in substantia nigra pars compacta (SNpc) region of brain. Oxidative stress and inflammation plays important role in the neurodegeneration and development of PD. Ursolic Acid (UA: 3β-hydroxy-urs-12-en-28-oic acid) is a natural pentacyclic triterpenoid found in various medicinal plants.