A fluorescent molecular rotor for biomolecular imaging analysis.

Biological macromolecules play a crucial role in information transmission, energy storage and structural support, and are closely related to the physiological state of living organisms. Disruption of the macromolecular homeostasis or anomalies of cellular microenvironment can precipitate cellular dysfunction, potentially leading to a spectrum of diseases including diabetes, neurodegenerative disorders, inflammatory conditions, and cancer. Consequently, aberrations in biological macromolecules have emerged as pivotal biomarkers linked to a myriad of diseases.

Mitochondria-targeted fluorescent probe for imaging viscosity in hepatic ischemia-reperfusion injury cell model.

The viscosity of the cell microenvironment is a parameter that affects cell physiological processes. A fluorescent probe X-V was designed to detect the viscosity changes of a hepatic ischemia-reperfusion injury (HIRI) cell model with high selectivity and sensitivity. The fluorescence emission wavelength is 615 nm and the Stokes shift can be up to 125 nm, which can be used not only for intracellular viscosity changes stimulated by different drugs but also for the detection of cell viscosity changes in the HIRI cell model.