Hypoxia-induced tumor cell-intrinsic PLAU activation drives immunotherapy resistance in collagenic lung adenocarcinoma.

Background: Hypoxia, as one of the hallmarks in the progression of solid tumors, has been established as critically important for tumor adaptive survival; however, its role in mediating tumor microenvironment remodeling in advanced lung adenocarcinoma (LUAD) remains incompletely defined.

Methods: Bioinformatics methods were conducted to establish a "hypoxia gene module" and perform molecular subtyping of advanced LUAD patients.Western blotting and immunohistochemistry analyses were performed to elucidate the role of PLAU-PLAUR (also known as uPA-uPAR) signaling activation in hypoxia-associated "Collagenic" LUAD development.

Genome-wide analysis of AP2/ERF transcription Factors in reveals their impact on orchid diversity.

is a significant traditional Chinese horticultural crop, valued both economically and ornamentally. The APETALA2/ethylene response factor (AP2/ERF) transcription factors play crucial roles in regulating growth, development, cell differentiation, and responses to both biotic and abiotic stresses in plants. However, the regulatory functions of AP2/ERF factors in remain poorly understood.

Genome-wide identification and functional characterization of LBD gene family in four Cymbidium species (Orchidaceae) and potential regulatory role of CsiLBD27 in floral development of Cymbidium sinense.

Background: The Lateral Organ Boundaries Domain (LBD) gene family, encompassing plant-specific LOB domain proteins, plays essential roles in various aspects of plant growth and development, and has continuously diversified its functions across numerous species. However, studies on LBD genes in the Orchidaceae family remain limited. To our knowledge, this is the first systematic investigation of the LBD gene family in Cymbidium, a genus that exhibits remarkable species and trait diversity within the Orchidaceae.

Genome-Wide Characterization, Comparative Analysis, and Expression Profiling of Genes Family in Four Species (Orchidaceae).

The SWEET (Sugar Will Eventually be Exported Transporters) protein family plays a key role in plant growth, adaptation, and stress responses by facilitating soluble sugar transport. However, their functions in remain poorly understood. This study identified 59 genes across four species, encoding conserved MtN3/saliva domains.

Identification of the "Collagen-Macrophage" sub-category of patients with colorectal cancer as an extension of the CMS4 subtype with THBS2 as a therapeutic target.

We identified a subset of patients with colorectal cancer (CRC) enriched with "collagen-TAMs," designated the CM class, using large integrated colon cancer transcriptome and single-cell transcriptome datasets. This classification system could be used as an extension of the traditional CMS classification system for CRC to guide more accurate classification and treatment.We also screened CAF-derived THBS2 as a potential biomarker for CM and found that it plays an important role in CRC disease models in vitro and in vivo, promoting tumor development and metastasis as well as TAM recruitment.

Centenary Progress on Orchidaceae Research: A Bibliometric Analysis.

Background: Research on orchids has experienced substantial growth since the early 20th century, reflecting their ecological and evolutionary significance.

Methods: This paper provides a comprehensive bibliometric analysis of orchid-related literature published between 1902 and 2024, based on data retrieved from the Web of Science Core Collection™ (WoS).

Results: The primary goal is to assess the global research landscape of orchids by identifying key authors, institutions, and journals, as well as major research themes in the field.

Phosphorylation of 399S at CsHsp70 of Cymbidium sinense is essential to maintain chlorophyll stability.

The Chinese orchids symbolise nobility and gentility in China, and the variation of leaf color makes Cymbidium sinense more diversified and valuable. However, its color variations especially at the protein level still remain largely unexplored. In this study, the proteomics and phosphoproteomics of Cymbidium sinense leaf color variation mutants were studied.

Integrated proteomic, transcriptomic, and metabolomic profiling reveals that the gibberellin-abscisic acid hub runs flower development in the Chinese orchid .

The seasonal flowering Chinese produce an axillary floral meristem and require a dormancy period during cold conditions for flower development. However, the bud activation mechanism remains elusive. This study evaluates the multi-omics across six stages of flower development, along with functional analysis of core genes to decipher the innate mechanism of floral bud initiation and outgrowth in the Chinese orchid .

Genome-Wide Identification, Expression, and Molecular Characterization of the Gene Family in Seven Orchid Species.

The orchid is one of the most distinctive and highly valued flowering plants. Nevertheless, the () gene family plays significant roles in the control of flowering, and its functions in Orchidaceae have been minimally explored. This research identified 68 potential genes within seven orchids' complete genome, divided into three groups (groups I, II, and III) via a phylogenetic tree.

Genome-wide association analysis identified molecular markers and candidate genes for flower traits in Chinese orchid ().

The orchid, the champagne of flowers, brings luxury, elegance, and novelty to nature. is a symbol of gigantic floral variability on account of wavering shapes and sizes of floral organs, although marker-trait association (MTA) has not been studied for its floral traits. We evaluated markers associated with 14 floral traits of through a genome-wide association study (GWAS) of 195 accessions.

Functional conservation and divergence of -like genes in floral development in .

is one of the most important traditional Chinese Orchids due to its unique and highly ornamental floral organs. Although the ABCDE model for flower development is well-established in model plant species, the precise roles of these genes in are not yet fully understood. In this study, four -like genes were isolated and identified from .

The Integrated mRNA and miRNA Approach Reveals Potential Regulators of Flowering Time in .

Orchids are among the most precious flowers in the world. Regulation of flowering time is one of the most important targets to enhance their ornamental value. The beauty of is its year-round flowering, although the molecular mechanism of this flowering ability remains masked.

Genome-wide identification of WRKY gene family and the importance of its Group III members in response to abiotic stress.

Transcription factors (TFs) of the WRKY family play pivotal roles in defense responses and secondary metabolism of plants. Although WRKY TFs are well documented in numerous plant species, no study has performed a genome-wide investigation of the WRKY gene family in . In the present work, we found 64 WRKY (CsWRKY) TFs, and they were further divided into eight subgroups.

The Transcriptome Profiling of Flavonoids and Bibenzyls Reveals Medicinal Importance of Rare Orchid .

Orchids are very important flowering plants that spend long juvenile phases before flowering. Along with aesthetic importance, they are rich sources of medicinal components. However, their long reproductive cycle is the major hurdle to study the medicinal efficacy.

Organ-Specific Gene Expression Reveals the Role of the 396 Module in Flower Development of the Orchid Plant .

Orchids are some of the most popular ornamental plants worldwide. Orchid floral morphology has increasingly attracted horticultural and commercial attention. Although multiple genes have been shown to be involved in the formation of the orchid flower, the underlying multi-level regulatory networks are largely unknown.

Genetic insights into the regulatory pathways for continuous flowering in a unique orchid Arundina graminifolia.

Background: Manipulation of flowering time and frequency of blooming is key to enhancing the ornamental value of orchids. Arundina graminifolia is a unique orchid that flowers year round, although the molecular basis of this flowering pattern remains poorly understood.

Results: We compared the A.

Stage Specificity, the Dynamic Regulators and the Unique Orchid .

Orchids take years to reach flowering, but the unique bamboo orchid () achieves reproductive maturity in six months and then keeps on year round flowering. Therefore, studying different aspects of its growth, development and flowering is key to boost breeding programs for orchids. This study uses transcriptome tools to discuss genetic regulation in five stages of flower development and four tissue types.

The genome of Cymbidium sinense revealed the evolution of orchid traits.

The Orchidaceae is of economic and ecological importance and constitutes ˜10% of all seed plant species. Here, we report a genome physical map for Cymbidium sinense, a well-known species belonging to genus Cymbidium that has thousands of natural variation varieties of flower organs, flower and leaf colours and also referred as the King of Fragrance, which make it arose into a unique cultural symbol in China. The high-quality chromosome-scale genome assembly was 3.

Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops.

Versatile protoplast platforms greatly facilitate the development of modern botany. However, efficient protoplast-based systems are still challenging for numerous horticultural plants and crops. Orchids are globally cultivated ornamental and medicinal monocot plants, but few efficient protoplast isolation and transient expression systems have been developed.

Transcriptome Analysis Reveals Clues into leaf-like flower mutant in Chinese orchid .

The floral morphology of , a well-known orchid in China, has increasingly attracted horticultural and commercial attention. However, the molecular mechanisms that regulate flower development defects in mutants are poorly understood. In this work, we examined a domesticated variety of named 'CuiYuMuDan', or leaf-like flower mutant, which lacks typical characteristics of orchid floral organs but continues to produce sepal-to leaf-like structures along the inflorescence.

Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in Orchids.

Protoplast systems have been proven powerful tools in modern plant biology. However, successful preparation of abundant viable protoplasts remains a challenge for orchids. Herein, we established an efficient protoplast isolation protocol from orchid petals through optimization of enzymatic conditions.

Identification and Characterization of NPR1 and PR1 Homologs in orchids in Response to Multiple Hormones, Salinity and Viral Stresses.

The plant () and () genes play fundamental roles in plant immunity response, as well as abiotic-stress tolerance. Nevertheless, comprehensive identification and characterization of and homologs has not been conducted to date in orchids, a valuable industrial crop cultivated as ornamental and medicinal plants worldwide. Herein, three -like (referred to as , , and ) and two ( and ) genes were genome-widely identified from orchids.

Comparative Metabolomic Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Leaf Color Development of 'Red Sun'.

The colorful leaf is an important ornamental character of (), especially the red leaf, which has always been attracted by breeders and consumers. However, little is documented on the formation mechanism of the red leaf of . In this study, the changing patterns of flavonoid-related metabolites, corresponding enzyme activities and genes expression in the leaves of 'Red Sun' from red to yellow and finally to green was investigated.

Low-temperature-induced changes in the transcriptome reveal a major role of CgSVP genes in regulating flowering of Cymbidium goeringii.

Background: Cymbidium goeringii is one of the most horticulturally important and popular ornamental plants in the orchid family (Orchidaceae). It blooms in winter during January-March and a period of low temperature is necessary for its normal flowering, otherwise there is flower bud abortion, which seriously affects the economic benefits. However, the molecular mechanism underlying winter-blooming behavior in C.