CD3ε of a pan T cell marker involved in mouse keratitis.

Aim: To explore whether CD3ε is involved in the adaptive immunity of () keratitis in mice and the role of innate and adaptive immunity in it.

Methods: Mice models of keratitis were established by intra-stromal injection and corneal epithelial scratching. Subconjunctival injections of natamycin, wedelolactone, LOX-1 inhibitor (poly I) or Dectin-1 inhibitor (laminarin) were used to treat mice with keratitis.

CCPG1 involved in corneal infection.

Aim: To investigate whether non-canonical autophagy transport receptor cell cycle progression 1 (CCPG1) is involved in the corneal antifungal immune response.

Methods: Human corneal epithelial cells (HCECs) and human myeloid leukemia mononuclear cells (THP-1) macrophages stimulated by () were used as cell models. The expression of CCPG1 mRNA was detected by qRT-PCR.

Thymol Protects against Aspergillus Fumigatus Keratitis by Inhibiting the LOX-1/IL-1β Signaling Pathway.

Objective: To explore the anti-inflammatory effects and mechanisms of action of thymol in Aspergillus fumigatus (A. fumigatus) keratitis.

Methods: The minimum inhibitory concentration of thymol against A.

Comparison of clinical outcomes after femtosecond laser keratomileusis in eyes with low or high myopia.

Aim: To compare the clinical results of femtosecond (FS) laser keratomileusis (LASIK) in high myopic patients and low myopic patients.

Methods: This study included 212 myopic eyes undergoing LASIK using a VisuMax 500kHz FS laser. All treated eyes were assigned to one of two groups according to preoperative manifest spherical refraction: low myopia group (A, >-4.

High-mobility group box1 as an amplifier of immune response and target for treatment in keratitis.

Aim: To determine the roles of high-mobility group box1 (HMGB1) in pro-inflammation, host immune response and its potential target for treatment in () keratitis.

Methods: Expression of HMGB1 was tested in C57BL/6 normal and infected corneas. Dual immunostaining tested co-expression of HMGB1 with TLR4 or LOX-1.

The expression of lacrimal androgen-binding proteins in mice keratitis.

Aim: To investigate the expression of lacrimal androgen-binding proteins (ABPs) in mice () keratitis.

Methods: mice model from different gender was developed by intra-stromal injection. The expression of lacrimal ABPs in lacrimal gland specimens from keratitis mice was detected by the quantitative polymerase chain reaction (qRT-PCR).

Early expression of PTX3 in infected rat cornea.

Aim: To investigate the expression of pentraxin 3 (PTX3) in rat corneal epithelium at the early stage of () infection.

Methods: A total of 50 Wistar rats were randomly divided into control group, Sham group and experimental group (fungal keratitis group, FK group). The right eye was chosen as the experiment one and infected by .

Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse keratitis.

Aim: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with () keratitis.

Methods: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of . Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10.

Production of interleukin-1β related to mammalian target of rapamycin/Toll-like receptor 4 signaling pathway during infection of the mouse cornea.

Aim: To elucidate the effect of rapamycin on regulating the production of interleukin (IL)-1β in ()-induced keratitis and to verify whether the expression of IL-1β in keratitis is associated with the mammalian target of rapamycin (mTOR)/Toll-like receptor 4 (TLR4) signaling pathway.

Methods: Fungal keratitis mouse models of susceptible C57BL/6 mice were established using . The mice were subsequently treated with rapamycin.

Boxb mediate BALB/c mice corneal inflammation through a TLR4/MyD88-dependent signaling pathway in keratitis.

Aim: To investigate whether high-mobility group box 1 (HMGB1) Boxb exacerbates BALB/c mice corneal immune responses and inflammatory through the Toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)-dependent signaling pathway in () keratitis.

Methods: The mice corneas were pretreated with phosphate buffer saline (PBS), Boxb before infection. The abdominal cavity extracted macrophages were pretreated with PBS, Boxb, TLR4 inhibitor (CLI-095), Dimethyl sulfoxide (DMSO) separately before hyphae stimulation.

Mincle in the innate immune response of mice fungal keratitis.

Aim: To investigate how macrophage inducible C-type lectin (Mincle) influences inflammation in mice fungal keratitis induced by ().

Methods: C57BL/6 mice were infected with after pretreated with Mincle agonist TDB or Mincle neutralizing antibody (MincleAb), taking DMSO or IgG as control group respectively. The cornea lesions were monitored with slit-lamp microscope and evaluated by clinical score.

Meta-analysis of the efficacy and safety of combined surgery in the management of eyes with coexisting cataract and open angle glaucoma.

Aim: To conduct a systematic review and quantitative Meta-analysis of the efficacy and safety of combined surgery for the eyes with coexisting cataract and open angle glaucoma.

Methods: We performed a systematic search of the related literature in the Cochrane Library, PubMed, EMBASE, Web of Science databases, CNKI, CBM and Wan Fang databases, with no limitations on language or publication date. The primary efficacy estimate was identified by weighted mean difference of the percentage of intraocular pressure reduction (IOPR%) from baseline to end-point, the percentage of number of glaucoma medications reduction from pre- to post-operation, and the secondary efficacy evaluations were performed by odds ratio (OR) and 95% confidence interval (CI) for complete and qualified success rate.

Role of vasoactive intestinal peptide in -infected cornea.

Aim: To investigate the anti-inflammatory role of vasoactive intestinal peptide (VIP) in () ketatitis.

Methods: Expression of VIP was tested by polymerase chain reaction (PCR) in C57BL/6 and BALB/c normal and infected corneas. C57BL/6 mice were pretreated with recombinant (r) VIP, while BALB/c mice were pretreated with VIP antagonist, and then infected with .

Expression of indoleamine 2,3-dioxygenase in a murine model of Aspergillus fumigatus keratitis.

Aim: To observe the presence and expression of indoleamine 2,3-dioxygenase (IDO) during the corneal immunity to Aspergillus fumigatus (A. fumigatus) in the murine models.

Methods: The murine model of fungal keratitis was established by smearing with colonies of A.

Expression of S100B during the innate immune of corneal epithelium against fungi invasion.

Aim: To explore the expression of S100B in corneal epithelial cells under Aspergillus stimulation both in vivo and in vitro.

Methods: Immortalized human corneal epithelial cells (HCECs) were exposed to inactive Aspergillus fumigatus (A. fumigatus) conidia at 0, 4, 8, 12, 16, and 24h respectively.

Comparison of cytotoxicity and wound healing effect of carboxymethylcellulose and hyaluronic acid on human corneal epithelial cells.

Aim: To investigate the cytotoxic effect on human corneal epithelial cells (HCECs) and the ability to faciliate corneal epithelial wound healing of carboxymethylcellulose (CMC) and hyaluronic acid (HA).

Methods: HCECs were exposed to 0.5% CMC (Refresh plus(®), Allergan, Irvine, California, USA) and 0.

Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells.

Aim: To evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF).

Methods: HCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h.

Expression of dectin-1 during fungus infection in human corneal epithelial cells.

Aim: To evaluate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus.

Methods: A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups.

Characteristics of ocular abnormalities in gout patients.

Aim: To characterize the clinical features of ocular surface in gout patients in coastal area of Shandong Province in China.

Methods: A total of 380 consecutive gout patients were examined from January 2011 to May 2011. According to the course of gout, patients were divided into group A (<5 years), B (5-10 years) and C (>10 years).

Regulation of interleukin 33/ST2 signaling of human corneal epithelium in allergic diseases.

Aim: To identify the function of ST2 and explore the role of IL-33/ST2 signaling in regulating the pro-allergic cytokine production in human corneal epithelial cells (HCECs).

Methods: Human corneal tissues and cultured primary HCECs were treated with IL-33 in different concentrations without or with different inhibitors to evaluate the expression, location and signaling pathways of ST2 in regulating production of pro-allergic cytokine and chemokine. The expression of mRNA was determined by reverse transcription and real time PCR, and protein production was measured by enzyme-linked immunosorbent assay (ELISA), immunohistochemical and immunofluorescent staining.

Dectin-1 expression at early period of Aspergillus fumigatus infection in rat's corneal epithelium.

Aim: To investigate the expression of dendritic cell-associated C-type lectin-1(dectin-1) at the early period of Aspergillus fumigatus infection in rat's corneal epithelium.

Methods: A total of 72 Wistar rats were randomly divided into three groups: A, B and C. The right eyes were chosen as experimental eyes.

Effect of corneal graft diameter on therapeutic penetrating keratoplasty for fungal keratitis.

Aim: To evaluate the effect of corneal graft diameter on therapeutic penetrating keratoplasty (PKP) for fungal keratitis.

Methods: A total of 116 patients (116 eyes) suffered from fungal keratitis underwent PKP at the Affiliated Hospital of Medical College Qingdao University from May 2006 to May 2010. They were divided into two groups according to the corneal graft diameter.

Nucleotide oligomerization domain 2 contributes to the innate immune response in THCE cells stimulated by Aspergillus fumigatus conidia.

Aim: To investigate the expression of nucleotide oligomerization domain 2 (NOD2) in the immortalized human corneal epithelial cell line (THCE), and its role in the innate immune response triggered by inactive Aspergillus fumigatus (Af) conidia.

Methods: The normal THCE cells were investigated as controls. After incubation with inactive Af conidia for 0.

Early expression of surfactant proteins D in Fusarium solani infected rat cornea.

Aim: To investigate the early expression of surfactant proteins D(SP-D) in Fusarium solani infected rat cornea.

Methods: Wistar rats were divided into group A, B and C randomly. The right eyes were chosen as the experiment one.