Amlodipine inhibits the proliferation and migration of esophageal carcinoma cells through the induction of endoplasmic reticulum stress.

Background: L-type calcium channels are the only protein channels sensitive to calcium channel blockers, and are expressed in various cancer types. The Cancer Genome Atlas database shows that the mRNA levels of multiple L-type calcium channel subunits in esophageal squamous cell carcinoma tumor tissue are significantly higher than those in normal esophageal epithelial tissue. Therefore, we hypothesized that amlodipine, a long-acting dihydropyridine L-type calcium channel blocker, may inhibit the occurrence and development of esophageal cancer (EC).

[Effects of TOFA on growth of Eca109 and KYSE-450 cells in human esophageal squamous cell carcinoma].

To investigate the effects of 5-tetradecanoxy 2-furanic acid (TOFA) on cell proliferation, cell cycle and apoptosis of esophageal squamous cell carcinoma (ESCC) cells. Eca-109 cells and KYSE-450 cells were divided into control group (DMSO) and experimental group (TOFA), respectively. The cells (4×10 cells/100 μl) were inoculated into 96-well plates with 5 multiple wells at each concentration.

[Influence of Substrate Exposure Level on ANAMMOX Microbial Activity and Biomass].

Substrate exposure levels are vital for the growth and metabolism of ANAMMOX microorganisms, and their effects on growth characteristics of ANAMMOX sludge during the enrichment process have been rarely reported. Using two continuous flow stirred reactors and the process of a gradually developing nitrogen load, the changes in biomass and activity, as well as nitrogen removal efficiency of the reactors were investigated under high substrate exposure level culture mode (R1:effluent NH-N and NO-N concentrations were 40-60 mg·L) and low substrate exposure level culture mode (R2:effluent NH-N and NO-N concentrations were 0-20 mg·L). The results showed that the high substrate exposure level culture mode was more beneficial to the improvement of nitrogen removal performance of the ANAMMOX reactor.

[Granular Characteristics of Anaerobic Ammonia Oxidation Sludge During the Recovery Process].

Operation instability has become one of the factors restricting the application of the anaerobic ammonia oxidation (ANAMMOX) process. Under the condition that the substrate is not suppressed, the effects of the substrate concentration on the granulation and activity of ANAMMOX granular sludge in the recovery process were studied by restoring the activity of ANAMMOX sludge, which was derived from early-stage operation instability of the continuous stirred tank reactor (CSTR). The results show that the activity of ANAMMOX sludge was recovered and the denitrification capacity increased significantly after 126 days of operation.

[Effect of Temperature and pH on Nitrogen Conversion in Feammox Process].

In recent years, the oxidation of NH using Fe(Ⅲ) as an electron acceptor under anaerobic conditions (Feammox) has received significant research attention. In this study, the effect of pH and temperature on nitrogen conversion during the Feammox process was studied through activity recovery of Feammox sludge acclimated by anaerobic ammonium oxidation (ANAMMOX) sludge. Results showed that after 40 d operation, activity of Feammox sludge was recovered.

[Inhibitory Effects of Phosphate and Recovery on a Nitrification System].

The effect of phosphate concentration on nitrification was studied by using a stabilization nitrosation system, which was started up in a continuous flow reactor by inoculating sludge from a municipal wastewater treatment plant. The results showed that the nitrification system was started successfully after operating for 14 days. The conversion rate of ammonia nitrogen reached 92.

[Analysis of CANON Process Start-up with Fiber Carrier].

A CANON reactor with fiber carrier was started up by seeding nitrification sludge and ANAMMOX sludge to study the operating characteristics of a fiber carrier. The results showed that total nitrogen removal load rose from 0.09 kg·(m·d) to 0.

[Influence of Phosphate on Nitrogen Removal Efficiency of ANAMMOX Sludge].

In this study, the effect of phosphate concentration on the nitrogen removal efficiency of ANAMMOX sludge was researched. The inhibition kinetic parameters were fitted and the abundance of ANAMMOX bacteria in the reactor before and after phosphate inhibition was analyzed by Real-time PCR. The short-term experiments showed that there was no significant effect on the nitrogen removal efficiency of ANAMMOX sludge when phosphate concentration was less than 30mg·L.

[Start-up and Capacity Enhancement of a Partial Nitrification Pilot Reactor in Continuous Flow].

The continuous flow reactor was used to treat simulated ammonia nitrogen wastewater by inoculating the sludge after filtration and adding a suspended filler. Regulations of free ammonia (FA), free nitrous acid (FNA), and dissolved oxygen (DO) in the reactor were the key to achieving a successful start-up of the pilot scale nitrosation reactor. The results show that the enrichment of ammonium oxidizing bacteria (AOB) and the elimination of nitrite oxidizing bacteria (NOB) are achieved by adjusting the operational mode of high DO, low DO, FA, and FNA in the reactor operation.

[Removal of Nitrogen from Alcohol Wastewater by PN-ANAMMOX].

An integrated partial nitrification anaerobic ammonia oxidation reactor was used to explore the feasibility of nitrogen removal from recycled ethanol wastewater. The results show that the integrated partial nitrification-anaerobic ammonia oxidation (PN-ANAMMOX) reactor was started successfully after 40 d under the conditions of pH 7.8±0.

[Ammoniac Waste Gas Treatment Based on ANAMMOX Process].

The feasibility of ammoniac waste gas treatment by partial nitrification(PN)-anaerobic ammonia oxidation(ANAMMOX) reactor was studied. The PN-ANAMMOX reactor was successfuly started up after 60 d operation in conditions of low dissolved oxygen control(0.2 mg·L), pH 7.

Berberine displays antitumor activity in esophageal cancer cells .

Aim: To investigate the effects of berberine on esophageal cancer (EC) cells and its molecular mechanisms.

Methods: Human esophageal squamous cell carcinoma cell line KYSE-70 and esophageal adenocarcinoma cell line SKGT4 were used. The effects of berberine on cell proliferation were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

High DEPTOR expression correlates with poor prognosis in patients with esophageal squamous cell carcinoma.

Objective: The disheveled, Egl-10, and pleckstrin (DEP) domain containing mammalian target of rapamycin (mTOR)-interacting protein (DEPTOR) is a binding protein containing mTOR complex 1 (mTORC1), mTOR complex 2 (mTORC2), and an endogenous mTOR inhibitor. DEPTOR shows abnormal expressions in numerous types of solid tumors. However, how DEP-TOR is expressed in esophageal squamous cell carcinoma (ESCC) remains elusive.

[Incorporation of TV regularization in deconvolution for partial volume correction in PET imaging].

We propose a method using total variation (TV) regularization in deconvolution for partial volume correction in PET imaging. In the degraded image model, we used TV regularization procedure in Van Cittert (VC) and Richardson-Lucy (RL) deconvolution algorithms. These methods were tested in simulated NCAT images and images of NEMA NU4-2008 IQ phantom and tumor-bearing mouse scanned by Simens Invoen microPET.