Validation study of spectrophotometric direct peptide reactivity assay (spectro-DPRA), a newly modified in chemico skin sensitization test method.

This study was conducted to evaluate the accuracy and reliability of the Spectro-DPRA, an enhanced method for the OECD TG 442C (direct peptide reactivity assay, DPRA), serving as an alternative to animal testing for skin sensitization. The validation of Spectro-DPRA was executed across four participating laboratories (Lab 1, Lab 2, Lab 3, and Lab 4, the latter only participating in the proficiency test) adhering to GLP principles. It covered transferability, proficiency, within laboratory and between laboratory reproducibility tests, and a predictive capacity test using 40 additional substances.

Optimal placement of inherent zeros in polynomial-based motion profiles for minimizing residual vibration and travel time under system uncertainties.

Various industrial processes require motion profiles that determine machine movement over time for workpiece transfer, with the goal of minimizing transfer time to enhance productivity. However, pursuing the maximum speed can trigger excessive motion-induced vibration, which can reduce the manufacturing efficiency, accuracy and operational lifespan of the system. This study proposes a generalized motion profile optimization method that considers the strategic placement of inherent zeros within the motion profile: each inherent zero contributes to the reduction of residual vibration or optimization of arrival time.

Analysis of the dislocation activity of Mg-Zn-Y alloy using synchrotron radiation under tensile loading.

An understanding of deformation behavior and texture development is crucial for the formability improvement of Mg alloys. X-ray line profile analysis using the convolutional multiple whole profile (CMWP) fitting method allows the experimental determination of dislocation densities separately for different Burgers vectors up to a high deformation degree. A wider use of this technique still requires exploration and testing of various materials.

Experimental development of levitation control for a high-accuracy magnetic levitation transport system.

In this paper, we present the experimental levitation control development in a high-accuracy magnetic levitation transport system. With this levitation control implementation, the input and output of sub-systems can be verified through a real-time system. The levitation control loop has a fast response, and the control algorithms are easily implemented.

Quorum sensing-dependent metalloprotease VvpE is important in the virulence of Vibrio vulnificus to invertebrates.

Vibrio vulnificus, a Gram-negative bacterium, is an opportunistic human pathogen responsible for fatal septicemia caused by contaminated sea foods in eastern Asia. Quorum sensing (QS) is a cell-density dependent gene regulation mechanism that controls the expression of many virulence genes in various bacteria and V. vulnificus has been also suggested to express their virulence genes through the QS system.

Interspecies signaling through QscR, a quorum receptor of Pseudomonas aeruginosa.

The QS machinery of Pseudomonas aeruginosa, an opportunistic human pathogen, consists of three acyl-homoserine lactone (acyl-HSL) signaling systems, LasR-I, RhlR-I, and QscR. QscR, known as an orphan receptor and a repressor of other QS systems, operates its own regulon using N-3-oxododecanoyl HSL (3OC12), which is synthesized by LasI, as its signal. In this study, we addressed the role of QscR in interspecies communication.

Growth phase-differential quorum sensing regulation of anthranilate metabolism in Pseudomonas aeruginosa.

Pseudomonas quinolone signal (PQS) plays a role in the regulation of virulence genes and it is intertwined in the las/rhl quorum sensing (QS) circuits of Pseudomonas aeruginosa. PQS is synthesized from anthranilate by pqsA-D and pqsH whose expression is influenced by the las/rhl systems. Since anthranilate can be degraded by functions of antABC and catBCA, PQS synthesis might be regulated by the balance between the expression of the pqsA-D/phnAB, pqsH, antABC, and catBCA gene loci.