Publications by authors named "Cassandra Bazile"
Article Synopsis
- * Current research seeks to understand T1D and develop new treatments, including immune system modulation, β-cell regeneration, and advanced insulin delivery methods like closed-loop pumps and stem cell therapies.
- * Recent studies suggest that TNF-α inhibitors could help preserve β-cell function in people with advanced T1D, prompting experts to convene and plan pivotal clinical trials to further evaluate their effectiveness.
While apoptosis plays a role in B-cell self-tolerance, its significance in preventing autoimmunity remains unclear. Here, we report that dysregulated B cell apoptosis leads to delayed onset autoimmune phenotype in mice. Our longitudinal studies revealed that mice with B cell-specific deletion of pro-apoptotic Bim ( ) have an expanded B cell compartment with a notable increase in transitional, antibody secreting and recently described double negative (DN) B cells.
View Article and Find Full Text PDFMany proteins regulate the expression of genes by binding to specific regions encoded in the genome. Here we introduce a new data set of RNA elements in the human genome that are recognized by RNA-binding proteins (RBPs), generated as part of the Encyclopedia of DNA Elements (ENCODE) project phase III. This class of regulatory elements functions only when transcribed into RNA, as they serve as the binding sites for RBPs that control post-transcriptional processes such as splicing, cleavage and polyadenylation, and the editing, localization, stability and translation of mRNAs.
View Article and Find Full Text PDFRNA binding proteins (RBPs) orchestrate the production, processing, and function of mRNAs. Here, we present the affinity landscapes of 78 human RBPs using an unbiased assay that determines the sequence, structure, and context preferences of these proteins in vitro by deep sequencing of bound RNAs. These data enable construction of "RNA maps" of RBP activity without requiring crosslinking-based assays.
View Article and Find Full Text PDFArticle Synopsis
- Around 12% of global human cancers are linked to infections from oncogenic viruses, including Kaposi's sarcoma herpesvirus (KSHV), which is associated with cancers like Kaposi's sarcoma and Primary Effusion Lymphoma (PEL).
- KSHV infection leads to chronic inflammation that facilitates cancer development, with the NF-κB transcription factor being persistently activated in infected cells through viral oncogenes vFLIP and vGPCR.
- The study identifies Cell Adhesion Molecule 1 (CADM1) as crucial for maintaining chronic NF-κB activation and survival of KSHV-infected cells, highlighting its interaction with vFLIP and vGPCR as key mechanisms in the virus
Article Synopsis
- Many RNA binding proteins (RBPs) interact with specific RNA sequences, but only 15%-40% of these motifs are actively bound in living organisms.
- A study using 12,000 mouse RNA sequences showed that the binding strength of RBPs like MBNL1 and RBFOX2 in laboratory settings correlated with their binding in real biological contexts, as well as their roles in development and splicing evolution.
- The primary factor influencing RBP binding is the RNA's secondary structure, with evidence that mutations improving motif accessibility can enhance protein binding and regulatory functions.