Analyzing RNA Localization Using the RNA Proximity Labeling Method OINC-seq.

Thousands of RNAs are localized to specific subcellular locations, and these localization patterns are often required for optimal cell function. However, the sequences within RNAs that direct their transport are unknown for almost all localized transcripts. Similarly, the RNA content of most subcellular locations remains unknown.

Big1 is a cell cycle regulator linking cell size to basal body number.

Cell size control in dividing cells coordinates cell growth with cell division. In the ciliated protozoan, , there is a tight link between cell size and the cytoskeletal assemblies at the cell cortex organized around basal bodies (BBs). BBs dictate the distribution of ciliary units governing cell motility and are organized into 18-22 ciliary rows.

The Unkempt RNA-binding protein reveals a local translation program in centriole overduplication.

Excess centrosomes cause defects in mitosis, cell-signaling, and cell migration, and therefore their assembly is tightly regulated. The divergent Polo kinase, PLK4, controls centriole duplication at the heart of centrosome assembly, and elevated PLK4 levels promote centrosome amplification (CA), a founding event of tumorigenesis. Here, we investigate the transcriptional consequences of elevated PLK4 and find Unkempt (UNK), a gene encoding an RNA-binding protein with roles in mRNA translational regulation, to be one of only two upregulated mRNAs.

Z-Form Stabilization By The Zα Domain Of Adar1p150 Has Subtle Effects On A-To-I Editing.

The role of Adenosine Deaminase Acting on RNA 1 (ADAR1)'s Z-conformation stabilizing Zα domain in A-to-I editing is unclear. Previous studies on Zα mutations faced limitations, including variable ADAR1p150 expression, differential editing analysis challenges, and unaccounted changes in ADAR1p150 localization. To address these issues, we developed a Cre-lox system in ADAR1p150 KO cells to generate stable cell lines expressing Zα mutant ADAR1p150 constructs.

Significantly Lower Incidence of Patellar Clunk Syndrome Using a Highly Congruent Tibial Insert.

Patellar crepitus and patellar clunk syndrome are potential complications seen in patients undergoing total knee arthroplasty (TKA). The etiology of this phenomenon is incompletely understood. A retrospective chart review was performed to identify a consecutive series of patients who underwent primary TKA with either a traditional posterior-stabilized implant (group 1, 728 TKAs) or a cruciate substituting implant (group 2, 393 TKAs).