Prevalence and Resistance Profiles of Pediatric Enterococcal Isolates: A Five-Year Update from Children's Medical Center Hospital, Tehran.

Background: In this study, attempts were made to evaluate the frequency of high-level gentamicin-resistant (HLGR) and vancomycin-resistant enterococci (VRE) and the prevalence and antibiotic resistance profile of enterococcal species isolated from pediatric patients referred to Children's Medical Center Hospital, Tehran, over five years.

Materials And Methods: A total of 404 enterococcal isolates from different patients referred to the Children's Medical Center between March 2016 and March 2021 were included in this cross-sectional study. Antimicrobial susceptibility testing was performed using standard methods according to the guidelines of the Clinical Laboratories Standards Institute (CLSI).

An overview of SARS-CoV2 natural infections in companion animals: A systematic review of the current evidence.

This systematic review provides a comprehensive overview of natural SARS-CoV-2 infections in companion animals. The findings show that these infections are relatively rare. Among the examined dogs, only 1.

Sub-minimum inhibitory concentrations (sub-MICs) of colistin on biofilm formation potency, adherence, and invasion to epithelial host cells: an experimental study in an Iranian children's referral hospital.

Here, we described the efficacy of colistin sub-minimum inhibitory concentrations (sub-MICs) on biofilm-forming activity, host epithelial cell adherence, and invasion capacity of strains collected from children admitted to the Children's Medical Center Hospital. Biofilm formation potency of clinical isolates was measured using a 96-well microtiter plate assay. Distribution of biofilm-related genes, including , , , , and , was detected by PCR.

candidemia and bacterial co-infection in a 3-month-old infant with biliary atresia.

Candidemia caused by rare and uncommon species is becoming more prevalent in pediatric healthcare settings, resulting in significant morbidity and mortality. One such species, , is resistant to fluconazole but highly susceptible to echinocandins. Here, we report the first documented case of candidemia in Iran that occurred in a male infant with biliary atresia who had been hospitalized for 2 months.

Evaluation of colonization index, molecular identification, and antifungal susceptibility pattern of species isolated from critically ill pediatric patients: A single-center study in Iran.

Background And Purpose: Given the high mortality rate of invasive candidiasis in hospitalized pediatric patients, it is crucial to establish a predictive system to achieve early diagnosis and treatment of patients who are likely to benefit from early antifungal treatment. This study aimed to assess the colonization index, species distribution, and antifungal susceptibility pattern of strains isolated from pediatric patients with high colonization index (CI).

Materials And Methods: This study was carried out at the Children's Medical Center in Tehran-Iran.

High frequency of colonization in critically ill pediatrics: A cross-sectional study in children's medical center, Tehran, Iran.

Background And Purpose: This study aimed to evaluate the species distribution and susceptibility pattern of the strains isolated from Candida colonization in pediatric patients staying at pediatric intensive care unit (ICU) and infant ICU of Children's Medical Center in Tehran, Iran.

Materials And Methods: This study was conducted in the Children's Medical Center in Tehran, Iran. In total, 440 samples from 56 patients with oral cavity, skin surrounded catheters, and ear, throat, nasal, and urine cultures were collected.

The first case of Wickerhamomyces anomalus fungemia in Iran in an immuneodeficient child, a review on the literature.

The incidence of invasive candidiasis in pediatric patients is increasing and is associated with significant morbidity and mortality. C. pelliculosa has been rarely reported as a human pathogen, however, it has been associated with serious nosocomial infections and clonal outbreaks with poor clinical outcomes in immunocompromised children were reported.

Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts.

Background: Multiple yeast species can cause human disease, involving superficial to deep-seated infections. Treatment of these infections depends on the accurate identification of causative agents; however, reliable methods are not available in many laboratories, especially not in resource-limited settings. Here, a new multiplex assay for rapid and low-cost identification of pathogenic yeasts is described.

South Asian (Clade I) Candida auris meningitis in a paediatric patient in Iran with a review of the literature.

Candida meningitis is a rare life-threatening yeast infection mostly involving immunocompromised or paediatric patients undergoing neurosurgical procedures or shunt placement. Due to difficulties in diagnosis because of diverse clinical manifestations, the number of patients affected is most likely underestimated. Therefore, the correct diagnosis may be delayed for months, and accurate species identification is highly recommended for administering appropriate antifungal therapy.

Prevalence of β-lactamase-encoding genes and molecular typing of Acinetobacter baumannii isolates carrying carbapenemase OXA-24 in children.

Background: β-Lactam antibiotics have been broadly used for the treatment of Acinetobacter baumannii infections, resulting in development of β-lactam inactivating β-lactamases. Here, we described antibiotic resistance rate, prevalence of β-lactamase-encoding genes, and clonal relationships of A. baumannii strains isolated from children referred to Children's Medical Center in Tehran, Iran, during 2019-2020.

Distribution of ciprofloxacin-resistance genes among ST131 and non-ST131 clones of isolates with ESBL phenotypes isolated from women with urinary tract infection.

Background And Objectives: sequence type 131 (ST131) is associated with extended-spectrum beta-lactamase (ESBL) production and fluoroquinolone resistance. This study aimed to investigate the prevalence of ST131, ESBL, and plasmid-mediated quinolone resistance (PMQR) genes in the ciprofloxacin-resistant (CIP) and ESBL producers from women with UTI.

Materials And Methods: The CIP-resistant ESBL producing (CIP/ESBL) isolates were screened for ST131-by specific PCR of and .

and as causes of pediatric candiduria: A study using gene size polymorphism.

Background: species cause a wide spectrum of disease entities. and -members of complex-are currently gaining both clinical and epidemiologic significance.

Materials And Methods: Totally, 150 pediatric isolates that had previously been identified as species complex based on a positive germ tube test were included.

Characterization of biofilm formation and virulence factors of isolates from paediatric patients in Tehran, Iran.

Objectives: can cause several infections. Its capability to form biofilm has been reported to be a vital property involved in the bacteria's pathogenesis. Various genes contributing to biofilm formation have not yet been completely clarified.

Distribution of Pathogenicity Island Markers and H-Antigen Types of O25b/ST131 Isolates from Patients with Urinary Tract Infection in Iran.

serogroup O25b-sequence type 131 ( O25b/ST131) is known as a multidrug-resistant organism with high virulence potential and has received attention internationally. We aim to investigate the prevalence of O25b/ST131 and the distribution of , pathogenicity island (PAI) markers, phylogenetic groups, and H-antigen typing in the O25b/ST131 isolated from patients with urinary tract infection (UTI) in Tehran, the capital of Iran. Seventy (26.

In Vitro Effects of Pumpkin () Seed Extracts on Protoscoleces.

Background: parasite causes a zoonotic disease which is important for public and veterinary health. Since pumpkin seeds ( sp.) are used as traditional vermifuge in Iran, they may be a potential herbal anthelmintic.

Serotype Distribution and Antibiotic Susceptibility of Isolates in Tehran, Iran: A Surveillance Study.

Introduction: Encapsulated strains cause high morbidity and mortality, mainly in countries with no pneumococcal conjugate vaccines (PCVs) immunization program. This study investigated the epidemiological changes of isolates including serotype distribution and antimicrobial susceptibility in Tehran, Iran.

Methods: A total of 80 samples were collected from patients admitted to Shariati hospital over two periods.

Involvement of in the occurrence of community and hospital acquired diarrhea, and its virulence diversity among the stool and the environmental samples.

Transmission of along the food chain could cause gastrointestinal infections. To show this involvement, the prevalence, putative virulence genotype, and antibiotic resistance phenotype of isolates from stool of 1482 patients with community and hospital acquired diarrhea were compared with 87 isolates from the environmental samples. The results showed infection with in 3.

Echinococcus multilocularis and Echinococcus granulosus in canines in North-Khorasan Province, northeastern Iran, identified using morphology and genetic characterization of mitochondrial DNA.

Background: Canids are definitive hosts of Echinococcus multilocularis and Echinococcus granulosus. This study aimed to survey these two Echinococcus species in canids of North-Khorasan Province, northeastern Iran, using morphological criteria and genetic characterization of mitochondrial DNA.

Methods: The carcasses of 106 canids, namely 61 jackals (Canis aureus), 23 foxes (Vulpes vulpes), 19 dogs (Canis familiaris) and three wolves (Canis lupus) were collected from the study area in 2013-2014 and examined for Echinococcus species.

Antimicrobial Resistance and Molecular Characteristics of Methicillin-resistant Isolates from Children Patients in Iran.

Introduction: Methicillin-resistant  (MRSA) causes high rates of mortality and a substantial burden to health systems worldwide. Here, we investigated the antimicrobial susceptibility and molecular characteristics of MRSA isolated from children referred to Children's Medical Center in Tehran.

Materials And Methods: A total of 98 MRSA isolates were collected from children.

Potential transmission sources of infection: detection of in various environmental samples.

Purpose: is one of the most common human infectious agents which may be transmitted via water. This study was designed to test presence via molecular methods in various aquatic environments as well as sewage sludge (SS) to understand the role of these environments in the pathogen's transmission.

Methods: specific primers for the 16S rRNA and A genes of were used in a nested and semi-nested PCR, respectively.

Species distribution and antifungal susceptibility profile of Candida isolates from blood and other normally sterile foci from pediatric ICU patients in Tehran, Iran.

As data on pediatric invasive candidiasis (IC) and the antifungal susceptibility pattern of associated isolates are scarce in Iran, this study aimed to determine species distribution and antifungal susceptibility profile of Candida species isolated from pediatric patients with suspected or documented IC. A total of 235 yeast strains recovered from normally sterile body fluids of patients admitted at the intensive care units of Children's Medical Centre, Tehran, Iran, were identified using CHROMagar Candida, molecular methods (ITS PCR-RFLP and sequencing), and MALDI-TOF. Susceptibility to amphotericin B, fluconazole, voriconazole, micafungin, and anidulafungin was determined according to the European on Antimicrobial Susceptibility testing reference microdilution method (EUCAST E.

Molecular analysis of accessory gene regulator functionality and virulence genes in Staphylococcus aureus derived from pediatric wound infections.

Staphylococcus aureus is a major human pathogen causing infections with high morbidity and mortality in both healthcare and community settings. The accessory gene regulator (Agr) is a key genetic element controlling the expression of numerous virulence factors in S. aureus.

Study on the relationship between the concentration and type of fungal bio-aerosols at indoor and outdoor air in the Children's Medical Center, Tehran, Iran.

Fungal bio-aerosols are of concern due to their adverse health effects, especially in indoor environments. The aim of this study was to evaluate the relationship between the concentration and type of fungal bio-aerosols in the indoor and outdoor of Children's Medical Center in Tehran, Iran. In the present descriptive-analytical study, the fungal bio-aerosols' concentrations in both indoor and outdoor of the hospital air were measured.

Rotavirus Infection Enhances Levels of Autoantibodies Against Islet Cell Antigens GAD65 and IA-2 in Children with Type 1 Diabetes.

Background: Some studies implicate rotavirus infection as a trigger for the development of type 1 diabetes mellitus (T1DM) in children, however findings are controversial.

Objectives: We investigated the link between rotavirus infection and autoantibodies against islet antigens and T1DM in children.

Methods: Serum samples from 80 new-onset diabetic and 80 nondiabetic children were screened for anti-rotavirus IgG, anti-GAD65 and anti-IA-2 autoantibodies using ELISA kits.