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Article Abstract
Background: species cause a wide spectrum of disease entities. and -members of complex-are currently gaining both clinical and epidemiologic significance.
Materials And Methods: Totally, 150 pediatric isolates that had previously been identified as species complex based on a positive germ tube test were included. The isolates were cultured on CHROMagar medium to ensure their purity and the results of germ tube test. For definitive speciation, PCR amplification and size polymorphism of the () gene was used. The results of -PCR were confirmed by sequencing the amplified fragments for randomly selected isolates of and .
Results: All 150 isolates included in this study were reconfirmed as complex on chromogenic media. Based on the gene size polymorphism, 141 (94%) isolates were identified as , 2 (1.33%) as , and 1 (0.67%) as . The remaining 6 (4%) complex isolates were a mix of and . All isolates of and have been recovered from cases of candiduria.
Conclusion: , either alone or mixed with , could be a cause of candiduria among pediatric patients and should not be ignored.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595836 | PMC |
| http://dx.doi.org/10.3934/microbiol.2020017 | DOI Listing |
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