Exploring omics strategies for drug discovery from isolated from the marine ecosystem.

Marine are prolific producers of diverse bioactive secondary metabolites, making them vital for drug discovery. Traditional cultivation and bioassay-guided isolation techniques often lead to the rediscovery of the same compounds, revealing the limitations of these traditional approaches and emphasizing the need for more advanced methods. The emergence of omics technologies such as genomics, metagenomics, transcriptomics, and metabolomics has dramatically enhanced the ability to investigate microorganisms by providing detailed insights into their biosynthetic gene clusters, metabolic pathways, and regulatory mechanisms.

Structural and functional properties of bacterial communities associated with rootless duckweed (Wolffia globosa) and their effect on the Wolffia growth.

Background: Rootless duckweed, Wolffia globosa, is emerging as a high-protein and starch biomass resource for various applications. However, the microbiomes and functional properties associated with Wolffia across a wide range of microbial sources remain largely unexamined. This study investigates the structure and functioning of the Wolffia microbiome and its impact on plant growth.

Phosphoproteomic insights into the regulation of root length in rice ( L. cv. KDML 105): uncovering key events and pathways involving phosphorylated proteins.

Root is a crucial organ in terrestrial plants, with the maximum root length (MRL) trait of the root system positively correlated with both plant growth and adaptation. However, the mechanisms regulating root length remain inadequately understood due to the dynamics of root growth. Protein phosphorylation precisely regulates various biological processes, providing a pathway to investigate the complex regulatory mechanisms of roots.

Reclassification of Microbacterium chocolatum Takeuchi and Hatano 1998 as a Later Heterotypic Synonym of Microbacterium aurantiacum Takeuchi and Hatano 1998.

The taxonomic position of Microbacterium chocolatum was evaluated through 16S rRNA gene sequence and genome-based analyses. The pairwise similarity of the 16S rRNA gene sequence retrieved from the genome data between Microbacterium aurantiacum KACC 20510 and Microbacterium chocolatum JCM 12412 was 99.3%, while between Microbacterium kitamiense JCM 10270 and M.

Phytoplasma DNA Enrichment from Sugarcane White Leaves for Shotgun Sequencing Improvement.

Sugarcane white leaf (SCWL) disease, caused by Phytoplasma sacchari, poses a significant threat to sugarcane cultivation. An obligate parasite, phytoplasma is difficult to culture in laboratory conditions, making the isolation of its DNA from the massive amount of plant host DNA extremely challenging. Yet, the appropriate amount and quality of plant microbiome-derived DNA are key for high-quality DNA sequencing data.

Transcriptomic Profiling of Sugarcane White Leaf (SCWL) Canes during Maturation Phase.

Sugarcane white leaf (SCWL) disease, caused by Phytoplasma sacchari, results in the most damage to sugarcane plantations. Some SCWL canes can grow unnoticed through the maturation phase, subsequently resulting in an overall low sugar yield, or they can be used accidentally as seed canes. In this work, 12-month-old SCWL and asymptomatic canes growing in the same field were investigated.

Description of sp. nov., an endophytic actinobacterium producing naphthomycin A and its genome insight for discovering bioactive compounds.

Endophytic actinobacteria are a group of bacteria living inside plant tissue without harmful effects, and benefit the host plant. Many can inhibit plant pathogens and promote plant growth. This study aimed to identify a strain of as a novel species and study its antibiotics production.

A new analog of dihydroxybenzoic acid from sp. KR21-0001.

Actinomycetes are well-known as the main producers of bioactive compounds such as antibiotics, anticancers, and immunosuppressants. Screening of natural products from actinomycetes has been an essential part of several drug discovery programs. Finding such novel biologically active metabolites is immensely important because of their beneficial health effects.

Genome-based reclassification of , and the genera , and .

In the present study, the taxonomic positions of , and members of the genera , and were evaluated. The 16S rRNA gene sequence similarity between DSM 14745, DSM 23947 and members of the genera and were above the cut-off level (>95 %) for genus delineation. Amino acid identity (AAI) values and the results of phylogenomic analysis suggested that and the members of the genera , and belong to the same genus.

Genome-based reclassification of Zillig . 1996 as a later heterotypic synonym of Schleper . 1996.

In the present study, we attempt to clarify the taxonomic positions of and . The 16S rRNA gene sequence similarity between DSM 9789 and DSM9790 (99.4 %) was above the threshold value (98.

Transcriptome Landscapes of Salt-Susceptible Rice Cultivar IR29 Associated with a Plant Growth Promoting Endophytic Streptomyces.

Plant growth-promoting endophytic (PGPE) actinomycetes have been known to enhance plant growth and mitigate plant from abiotic stresses via their PGP-traits. In this study, PGPE Streptomyces sp. GKU 895 promoted growth and alleviated salt tolerance of salt-susceptible rice cultivar IR29 by augmentation of plant weight and declined ROS after irrigation with 150 mM NaCl in a pot experiment.

Actinobacteria-Plant Interactions in Alleviating Abiotic Stress.

Abiotic stressors, such as drought, flooding, extreme temperature, soil salinity, and metal toxicity, are the most important factors limiting crop productivity. Plants use their innate biological systems to overcome these abiotic stresses caused by environmental and edaphic conditions. Microorganisms that live in and around plant systems have incredible metabolic abilities in mitigating abiotic stress.

Dynamic Alteration of Microbial Communities of Duckweeds from Nature to Nutrient-Deficient Condition.

Duckweeds live with complex assemblages of microbes as holobionts that play an important role in duckweed growth and phytoremediation ability. In this study, the structure and diversity of duckweed-associated bacteria (DAB) among four duckweed subtypes under natural and nutrient-deficient conditions were investigated using V3-V4 16S rRNA amplicon sequencing. High throughput sequencing analysis indicated that phylum Proteobacteria was predominant in across duckweed samples.

Transfer of Bacillus tepidiphilus Narsing Rao et al. 2021 to the genus Peribacillus as Peribacillus tepidiphilus comb. nov.

In the present study, the taxonomic position of Bacillus tepidiphilus was re-evaluated. Bacillus tepidiphilus (B. tepidiphilus) SYSU G01002 showed the highest 16S rRNA gene sequence similarity with the type strain Peribacillus alkalitolerans (P.

sp. nov., an endophytic actinobacterium isolated from the surface-sterilized stem of a Thai medicinal plant, Burm. f.

An endophytic actinobacterium, strain CLES2, was discovered from the surface-sterilized stem of a Thai medicinal plant, Burm. f., collected from the Phujong-Nayoa National Park, Ubon Ratchathani Province, Thailand.

Draft genome sequence of sp. K4S16 and elucidation of the nonthmicin biosynthetic pathway.

sp. K4S16 (=NBRC 110471) is a producer of a novel tetronate polyether compound nonthmicin. Here, we report the draft genome sequence of this strain together with features of the organism and assembly, annotation and analysis of the genome sequence.

Sarpeptins A and B, Lipopeptides Produced by sp. KO-7888 Overexpressing a Specific SARP Regulator.

Whole genome analysis of sp. KO-7888 has revealed various pathway-specific transcriptional regulatory genes associated with silent biosynthetic gene clusters. A antibiotic regulatory protein gene, , located adjacent to a novel nonribosomal peptide synthetase (NRPS) gene cluster, was overexpressed in the wild-type strain.

Gordonia oryzae sp. nov., isolated from rice plant stems (Oryza sativa L.).

A novel endophytic actinomycete, designated strain RS15-1S, was isolated from surface-sterilized stems of Oryza sativa L. collected from Sisaket province, Thailand. The colony of strain was strong orange, catalase-positive and oxidase-negative.

Modulation of salt tolerance in Thai jasmine rice (Oryza sativa L. cv. KDML105) by Streptomyces venezuelae ATCC 10712 expressing ACC deaminase.

1-Aminocyclopropane-1-carboxylate (ACC) deaminase is a plant growth promoting (PGP) trait found in beneficial bacteria including streptomycetes and responsible for stress modulation. The ACC deaminase gene, acdS, of S. venezuelae ATCC 10712 was cloned into an expression plasmid, pIJ86, to generate S.

Molecular Communications Pulse-Based Jamming Model for Bacterial Biofilm Suppression.

Studies have recently shown that the bacteria survivability within biofilms is responsible for the emergence of superbugs. The combat of bacterial infections, without enhancing its resistance to antibiotics, includes the use of nanoparticles to quench the quorum sensing of these biofilm-forming bacteria. Several sequential and parallel multi-stage communication processes are involved in the formation of biofilms.

Enhancement of the efficiency of Cd phytoextraction using bacterial endophytes isolated from Chromolaena odorata, a Cd hyperaccumulator.

Phytoextraction is a technique using a hyperaccumulator to remove heavy metals from soil. The efficiency of heavy metal uptake can be enhanced by the inoculation of endophytes. In this study, we isolated and identified 23 endophytes from Chromolaena odorata, a cadmium (Cd) hyperaccumulator that consisted of 19 bacteria, 2 actinomycetes and 2 fungi.

Actinomadura barringtoniae sp. nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn.

A novel actinomycete strain, designated GKU 128, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach.