Polyphasic discrimination of from closely related species and a whole-genome multilocus (wgMLST) scheme for the evaluation of diversity within this clade.

is an environmentally ubiquitous genus with significant roles in bioelectrochemical applications and human infections. However, identification problems involving , , and have been reported, potentially hindering research progress in these areas. In this study, we explored how to discriminate between these species.

Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic (ETEC) clones circulating in aquatic environments and in patients.

Diarrheal disease pathogens often spread through water-borne routes. Enterotoxigenic (ETEC) is a major bacterial agent causing diarrheal disease in children, adults, and travelers in endemic areas. In addition, ETEC is responsible for outbreaks of water and food-borne gasteroenteritis globally, ETEC isolates also show robust survival capacity in various environmental settings, including aquatic environments.

A hotspot of diversity: novel species isolated from Baltic Sea sediments delineate a sympatric species complex.

Two bacterial strains, SP1S1-4 and SP2S1-2, were isolated from sediment samples collected in the Stockholm archipelago in November 2021. Following whole-genome sequencing, these strains were identified as tentatively belonging to two novel genospecies, based on digital DNA-DNA hybridization, as implemented in the Type Strain Genome Server. , and were, in this order and within a narrow genomic relatedness range, their closest genotypic relatives.

Co-occurrence of ST412 isolates with hypermucoviscous and non-mucoviscous phenotypes in a short-term hospitalized patient.

Unlabelled: Hypermucoviscosity (HMV) is a phenotype that is commonly associated with hypervirulence in . The factors that contribute to the emergence of HMV subpopulations remain unclear. In this study, eight strains were recovered from an inpatient who had been hospitalized for 20 days.

Bacteriophage-resistant carbapenem-resistant Klebsiella pneumoniae shows reduced antibiotic resistance and virulence.

Phage therapy has shown great promise in the treatment of bacterial infections. However, the effectiveness of phage therapy is compromised by the inevitable emergence of phage-resistant strains. In this study, a phage-resistant carbapenem-resistant Klebsiella pneumoniae strain SWKP1711R, derived from parental carbapenem-resistant K.

sp. nov. and sp. nov., isolated from Baltic Sea water and sediments.

Two bacterial strains, SP1W3 and SP1S2-7, were isolated from samples of water and sediments collected in Vaxholm, a town located on the Stockholm archipelago in the Baltic Sea, in November 2021. The strains were identified as novel genomic species within the genus , based upon comparative analysis of whole genome sequence data. Strain SP1W3 (genome size, 5.

Coexistence of tigecycline resistance genes with two novel -carrying and -carrying transposons in a plasmid.

Introduction: Tigecycline and carbapenems are considered the last line of defense against microbial infections. The co-occurrence of resistance genes conferring resistance to both tigecycline and carbapenems in was not investigated.

Methods: A28 was isolated from hospital sewage.

Subinhibitory antibiotic concentrations promote the horizontal transfer of plasmid-borne resistance genes from to .

Horizontal gene transfer plays an important role in the spread of antibiotic resistance, in which plasmid-mediated conjugation transfer is the most important mechanism. While sub-minimal inhibitory concentrations (sub-MIC) of antibiotics could promote conjugation frequency, the mechanism by which sub-MIC levels of antibiotics affect conjugation frequency is not clear. Here, we used SW1780 carrying the multi-drug resistance plasmid pSW1780-KPC as the donor strain, to investigate the effects of sub-MICs of meropenem (MEM), ciprofloxacin (CIP), cefotaxime (CTX), and amikacin (AK) on conjugational transfer of pSW1780-KPC from SW1780 to J53.

Respiration-induced biofilm formation as a driver for bacterial niche colonization.

Depending on their physiology and metabolism, bacteria can carry out diverse redox processes for energy acquisition, which facilitates adaptation to environmental or host-associated niches. Of these processes, respiration, using oxygen or alternative terminal electron acceptors, is energetically the most favorable in heterotrophic bacteria. The biofilm lifestyle, a coordinated multicellular behavior, is ubiquitous in bacteria and is regulated by a variety of intrinsic and extrinsic cues.

Isolation and Characterization of vB_kpnM_17-11, a Novel Phage Efficient Against Carbapenem-Resistant .

Phages and phage-encoded proteins exhibit promising prospects in the treatment of Carbapenem-Resistant (CRKP) infections. In this study, a novel phage vB_kpnM_17-11 was isolated and identified by using a CRKP host. vB_kpnM_17-11 has an icosahedral head and a retractable tail.

sp. nov., a bacterium isolated from river water used for household purposes in Boane District, Mozambique.

A Gram-negative rod with a single polar flagellum was isolated from a freshwater reservoir used for household purposes in Boane District, near Maputo, Mozambique, and designated as strain DB1. Growth was observed at 30-42 °C (optimum, 30-37 °C) and with 0.5-1.

Comparative Genomics of Cyclic di-GMP Metabolism and Chemosensory Pathways in Shewanella algae Strains: Novel Bacterial Sensory Domains and Functional Insights into Lifestyle Regulation.

spp. play important ecological and biogeochemical roles, due in part to their versatile metabolism and swift integration of stimuli. While spp.

Complete Genome Sequence and Methylome of the Type Strain of Shewanella algae.

We report the complete genome sequence and base modification analysis of the Shewanella algae type strain CECT 5071 (= OK-1 = ATCC 51192 = DSM 9167 = IAM 14159). The genome is composed of a single chromosome of 4,924,764 bp, with a GC content of 53.10%.

Regulation of colony morphology and biofilm formation in Shewanella algae.

Bacterial colony morphology can reflect different physiological stages such as virulence or biofilm formation. In this work we used transposon mutagenesis to identify genes that alter colony morphology and cause differential Congo Red (CR) and Brilliant Blue G (BBG) binding in Shewanella algae, a marine indigenous bacterium and occasional human pathogen. Microscopic analysis of colonies formed by the wild-type strain S.

Reduction of alternative electron acceptors drives biofilm formation in Shewanella algae.

Shewanella spp. possess a broad respiratory versatility, which contributes to the occupation of hypoxic and anoxic environmental or host-associated niches. Here, we observe a strain-specific induction of biofilm formation in response to supplementation with the anaerobic electron acceptors dimethyl sulfoxide (DMSO) and nitrate in a panel of Shewanella algae isolates.

A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid.

The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E.

Nitrate Metabolism Modulates Biosynthesis of Biofilm Components in Uropathogenic and Acts as a Fitness Factor During Experimental Urinary Tract Infection.

To successfully colonize a variety of environments, bacteria can coordinate complex collective behaviors such as biofilm formation. To thrive in oxygen limited niches, bacteria's versatile physiology enables the utilization of alternative electron acceptors. Nitrate, the second most favorable electron acceptor after oxygen, plays a prominent role in the physiology of uropathogenic (UPEC) and is abundantly found in urine.

Whole-Genome Sequencing Redefines Taxonomy.

The genus encompasses a diverse group of Gram negative, primarily aquatic bacteria with a remarkable ecological relevance, an outstanding set of metabolic features and an emergent clinical importance. The rapid expansion of the genus over the 2000 s has prompted questions on the real taxonomic position of some isolates and species. Recent work by us and others identified inconsistencies in the existing species classification.

The 9-Fluoren Vinyl Ether Derivative SAM461 Inhibits Bacterial Luciferase Activity and Protects From Luminescent Vibriosis.

is a major pathogen in aquaculture. It is a causative agent of the so-called "luminescent vibriosis," a life-threatening condition caused by bioluminescent spp. that often involves mass mortality of farmed shrimps.

Multilocus sequence typing of Shewanella algae isolates identifies disease-causing Shewanella chilikensis strain 6I4.

The genus Shewanella is rapidly expanding, with new species being discovered frequently. Four species have been identified as pathogenic to humans, with Shewanella algae being most relevant. Evaluation of the clinical significance of Shewanella spp.

spp. infections in Gran Canaria, Spain: retrospective analysis of 31 cases and a literature review.

Introduction: spp. can cause severe skin and soft-tissue infections, gastrointestinal infections, otitis and bacteraemia, generally upon contact with seawater or consumption of raw seafood. Recently, a new condition termed 'patera foot' characterized by acute skin and soft-tissue infection has been described in irregular immigrants arriving to the Canary Islands, Spain, in rudimentary boats.

In vitro interactions of Acanthamoeba castellanii Neff and Vibrio harveyi.

Free-living amoebae (FLA) are opportunistic protozoa widely distributed in the environment. They are frequently found in water and soil samples, but they have also been reported to be associated with bacterial human pathogens such as Legionella spp. Campylobacter spp or Vibrio cholerae among others.