Rogueing or Rescuing? A Potential New Management Approach for Roses Infected with Rose Rosette Virus.

Roses ( spp.) are among the most economically and culturally significant flowering plants worldwide. However, rose cultivation faces a critical threat from rose rosette disease (RRD), which is caused by (rose rosette virus, RRV), a negative-sense RNA virus transmitted by the eriophyid mite .

Myricetin Amplifies Glucose-Stimulated Insulin Secretion via the cAMP-PKA-Epac-2 Signaling Cascade.

: Myricetin, a natural bioflavonoid, is reported as an anti-diabetic agent since it possesses the ability to inhibit α-glucosidase activity, stimulate insulin action and secretion, manage ROS, and prevent diabetes complications. Myricetin was identified as a new insulin secretagogue that enhances glucose-stimulated insulin secretion and seems like a better antidiabetic drug candidate. Here, we explored the insulinotropic mechanism(s) of myricetin in mice islets and .

Marine Fungi-Derived Compounds as Promising KRas Localization Blockers: Structural Insights Into PDE6δ Inhibition.

Cancer, often driven by genetic mutations like KRas activation, is a leading global cause of mortality. This study identified potential inhibitors of PDE6δ, a key chaperone required for KRas membrane localization, from marine fungi-derived compounds. Through molecular docking, molecular dynamics (MD) simulations, MMGBSA free energy calculations, and free energy landscape (FEL) analyses, three promising compounds were identified: CMNPD29003, CMNPD9449, and CMNPD30443.

Nuclearporin subcomplex Nup98/Rae1 is vital for maternal-to-zygotic transition during early embryonic development.

The Nup98/Rae1 nuclear pore subcomplex, a critical mediator of nucleocytoplasmic transport, orchestrates fundamental cellular processes including transcriptional regulation, mRNA surveillance, and mitotic fidelity. However, its functional significance during early embryogenesis remains incompletely understood. In this study, we employed an in vitro embryo culture system combined with embryo electroporation-mediated interference to investigate the role of Nup98/Rae1 in early mouse embryos.

Insights into the roles of chromatin-remodeling complex HOS15-PWR-HDA9 in plant development and stress responses.

This review highlights the central role of the HOS15-PWR-HDA9 complex in integrating chromatin remodeling with plant development and stress adaptation, offering insights for improving crop resilience. Being sessile by nature, plants have signaling pathways that perceive and transmit environmental signals, inducing epigenetic modifications that are instrumental in plant development and stress responses. Acetylation and methylation are key features of chromatin remodeling and are crucial in transcriptional reprogramming for tissue-specific gene expression and silencing.

Transmission dynamics and stability analysis of fractional HIV and AIDS epidemic model with antiretroviral therapy.

Worldwide populations have historically experienced serious issues from infectious diseases, requiring coordinated and inclusive prevention measures. HIV is one of the most hazardous of these as it attacks CD4 + cells, or T-cell lymphocytes, which are crucial to human immunity. To explore the variability of HIV/AIDS transmission, this study introduces a nonlinear stochastic mathematical model that incorporates a recovery compartment to account for hospitalized patients' progression to complete recovery and to better capture the intricate dynamics of disease transmission.

γ-Aminobutyric acid: An emerging biomolecule for maintaining the postharvest quality of fresh fruits and vegetables: A meta-analysis.

The preservation of fresh fruits and vegetables (F&V) is important to achieve food and nutritional security, reduce food loss, and boost global trade and economic growth. To understand the γ-Aminobutyric acid (GABA) impact on F&V quality, this study conducted a meta-analysis of 30 articles (2020-2024). GABA was found to effectively preserve F&V quality by regulating total soluble solids (TSS), delaying firmness loss, and minimizing browning, microbial spoilage, and chilling injury.

-Regulated Enhances Salt Tolerance in by Modulating Flavonoid Biosynthesis and Reactive Oxygen Species Scavenging.

High-salinity stress induces severe oxidative damage in plants, leading to growth inhibition through cellular redox imbalance. Chalcone synthase (CHS), a pivotal enzyme in the flavonoid biosynthesis pathway, plays critical roles in plant stress adaptation. However, the molecular mechanisms underlying CHS-mediated salt tolerance remain uncharacterized in L.

Blocking the LRH-1/LCN2 axis by ML-180, an LRH-1 inverse agonist, ameliorates osteoarthritis via inhibiting the MAPK pathway.

Osteoarthritis (OA) is a chronic and degenerative disease marked by inflammation and extracellular matrix (ECM) degeneration, contributing to synovial inflammation and cartilage destruction. Accumulating evidence has demonstrated that Liver receptor homolog-1 (LRH-1), an orphan nuclear receptor, mediates inflammatory response. However, there is a lack of evidence regarding the regulatory role of LRH-1 in OA pathogenesis.

Optimizing skin cancer screening with convolutional neural networks in smart healthcare systems.

Skin cancer is among the most prevalent types of malignancy all over the global and is strongly associated with the patient's prognosis and the accuracy of the initial diagnosis. Clinical examination of skin lesions is a key aspect that is important in the assessment of skin disease but comes with some drawbacks mainly with interpretational aspects, time-consuming and healthare expenditure. Skin cancer if detected early and treated in time can be controlled and its deadly impacts arrested completely.

HOS15 impacts DIL9 protein stability during drought stress in Arabidopsis.

HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15) acts as a substrate receptor of E3 ligase complex, which plays a negative role in drought stress tolerance. However, whether and how HOS15 participates in controlling important transcriptional regulators remains largely unknown. Here, we report that HOS15 physically interacts with and tightly regulates DROUGHT-INDUCED LIKE 19 (DIL9) protein stability.

Global insights and advances in edible coatings or films toward quality maintenance and reduced postharvest losses of fruit and vegetables: An updated review.

Transitioning to safe, nonthermal, and edible strategies for maintaining fruit and vegetable (F&V) quality, reducing postharvest losses (up to 55% annually), and ensuring food security requires extensive research and innovation in postharvest technologies. This review aims to provide an updated understanding of edible coatings or films (ECF), focusing on their role in reducing F&V postharvest losses, based on data from the last 40 years retrieved from the Web of Science database. The global ECF research network is represented by publication trends, majorly researched F&V, key research areas, influential and emerging authors, and global research ranking.

Comparison of Research Knowledge in Postgraduate Trainees of Clinical and Basic Health Sciences of a Tertiary Healthcare Setup: A Qualitative and Quantitative Assessment.

Background:  All recent advances in healthcare, including diagnostics, surveillance, management, and disease prevention, have depended on good-quality research that has brought new information to light. Therefore, in Pakistan, it is important to develop good research skills as, for many years, our physicians have relied on research knowledge from the Western world, which does not necessarily provide solutions to a developing country. Considering the gap in research knowledge among young doctors, the study was planned to compare the research knowledge of postgrad trainees of clinical and basic health sciences (BHS) of private tertiary care hospitals in Karachi.

Randomised controlled trials of dual antiplatelet therapy versus aspirin in patients with stroke or transient ischaemic attack: an updated meta-analysis.

Introduction: We explored the efficacy and safety of dual antiplatelet therapy (DAPT) for individuals diagnosed with stroke or transient ischaemic attack (TIA), incorporating the latest insights from randomised controlled trials (RCTs). The emerging evidence surrounding DAPT in stroke and TIA plays a pivotal role in guiding clinical decisions.

Methods: Our study included five RCTs (INSPIRES, THALES, POINT, CHANCE, FASTER) on DAPT (aspirin + P2Y12 inhibitor) initiated within 72 hours of acute stroke or TIA, which evaluated DAPT efficacy and safety over 21-90 days, focusing on new strokes and major bleeding.

Comprehensive Genome-Wide Identification and Expression Profiling of Transcription Factors in Under Abiotic Stress.

The basic helix-loop-helix () transcription factor (TF) family, the second-largest among eukaryotes, is known for its evolutionary and functional diversity across plant species. However, genes have not yet been characterized in . In this study, we identified 76 genes, which exhibit a variety of physicochemical properties.

Integrative Network Pharmacology, Molecular Docking, and Dynamics Simulations Reveal the Mechanisms of in Diabetic Nephropathy Treatment: An In Silico Study.

Diabetic nephropathy (DN) is a serious diabetes-related complication leading to kidney damage. (CT), traditionally used in managing diabetes and kidney disorders, has shown potential in treating DN, although its active compounds and mechanisms are not fully understood. This study aims to identify CT's bioactive compounds and explore their therapeutic mechanisms in DN.

Mitochondrial regulation in the tumor microenvironment: targeting mitochondria for immunotherapy.

Mitochondrial regulation plays a crucial role in cancer immunity in the tumor microenvironment (TME). Infiltrating immune cells, including T cells, natural killer (NK) cells, and macrophages, undergo mitochondrial metabolic reprogramming to survive the harsh conditions of the TME and enhance their antitumor activity. On the other hand, immunosuppressive cells like myeloid-derived suppressor cells (MDSCs), regulatory T cells (Tregs), mast cells, and tumor-associated macrophages (TAMs) rely on mitochondrial regulation to maintain their function as well.

Introgression and Mapping of Cotton Leaf Curl Disease Resistance from Wild Kearney into Upland Cotton (. ).

Cotton leaf curl disease (CLCuD), caused by the whitefly transmitted geminivirus complex (cotton leaf curl virus [CLCuV] and their satellite molecules), is a serious threat to successful upland cotton production in northwest India and Pakistan. The disease causes significant losses in fiber yield and the quality of cotton. Owing to the regular emergence of resistance-breaking strains of CLCuV, all the previously available CLCuD-resistant germplasms of upland cotton have become compromised, and none of the extant upland cotton cultivars is resistant to this disease.

Clinical and Molecular Profile of Duchenne Muscular Dystrophy (DMD): Case-Record Analysis From Uttar Pradesh, India.

Objective: To assess the clinical and molecular profile of patients with Duchenne Muscular Dystrophy (DMD) presenting to a tertiary center in Eastern Region of Uttar Pradesh, India.

Methods: In this retrospective study, case records of all patients diagnosed as DMD were analyzed to ascertain the clinical phenotype and molecular profile. Multiplex polymerase chain reaction (mPCR) technique, Multiplex Ligation Dependent Probe Amplification (MLPA) and Next Generation Sequencing (NGS) were used for establishing the molecular diagnosis.

Forecasting the Pharmacological Mechanisms of and in Diabetic Retinopathy Treatment: A Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation Study.

(1) Background: Diabetic retinopathy (DR) is a major complication of diabetes, marked by abnormal angiogenesis, microaneurysms, and retinal hemorrhages. Traditional Ayurvedic medicine advocates multi-target strategies for DR management. However, the mechanisms by which (SX) and (PZ) exert therapeutic effects are not well understood; (2) Methods: To investigate these mechanisms, we employed network pharmacology and molecular docking techniques.

Effect of flaxseed oil cyclolinopeptides on lipid oxidation, protein oxidation, and lipid profile during in vitro digestion of high-fat beef.

This study investigated the influence of flaxseed oil cyclolinopeptides (CLs) on lipid and protein oxidation during high-fat meat digestion. Fourteen CLs were identified in flaxseed oil through UHPLC-ESI-QTOF-MS/MS, with dominant CLA, CLB, CLE, and CLM. During in vitro digestion, CLs inhibited lipid oxidation products (lipid hydroperoxide, Malondialdehyde, and 4-hydroxynonenal) and protein carbonylation.

Genetic Profile of Beta-Thalassemia and Sickle Cell Disease in Eastern Uttar Pradesh.

We report the prevalence of different mutations in the hemoglobin subunit beta (HBB) gene of 133 children with beta-thalassemia and 23 children with sickle cell disease (SCD), most of them belonging to the states of Uttar Pradesh (UP), Jharkhand, Chhattisgarh and Bihar. IVS 1-5 was the most common mutation (n = 42) followed by CD41/42 (n = 4) and CD8/9 (n = 4). Notably, some mutations like c.

Revolutionising infection control: building the next generation of phage banks.

The escalating global burden of antimicrobial resistance (AMR) represents a critical public health challenge. This rise in antibiotic resistance is concomitant with heightened antibiotic consumption, with an estimated annual usage of 100,000 to 200,000 tons. A recent systematic review, which analysed data from 204 countries, reported that AMR was responsible for 4.

Phytochemical Composition and Toxicological Screening of Anise Myrtle and Lemon Myrtle Using Zebrafish Larvae.

Plants are an immense source of drugs, and 50% of modern pharmacopeia has a plant origin. With increasing life expectancy in humans, many age-related degenerative diseases converge on oxidative cellular stress pathways. This provides an opportunity to develop broad treatments by targeting the cause of common pathologic cell degeneration.