Efficient Immobilization of Lipase for Sustainable Lipid Degradation and Wastewater Bioremediation.

Immobilized lipases are emerging as highly effective biocatalysts due to their enhanced operational stability, reusability, and promising environmental applications, particularly in the bioremediation of lipid-contaminated wastewater. In this study, the lipase from (Lip) was immobilized on various supports, with calcium carbonate demonstrating the highest immobilization efficiency (82.66 ± 5.

Structural insights and biomedical potential of biosynthesized silver nanoparticles: antibacterial activity, anti-biofilm and cancer cell inhibition.

Background: The increasing threat of antimicrobial resistance and cancer has driven the search for new therapeutic agents, with plant-based biosynthesis of nanoparticles emerging as a promising approach. Silver nanoparticles (AgNPs) synthesized from plant extracts have gained attention for their potential biomedical applications.

Objective: This study aimed to synthesize, characterize, and evaluate the antimicrobial, anti-biofilm, and anticancer properties of AgNPs derived from (Tp), (Tm), and (Pg).

New Nitrogen-, Oxygen-, and Sulfur-Containing Heterocyclic Compounds as Anti-Colon Cancer Agents: Synthesis, Multitargeted Evaluations, Molecular Docking Simulations and ADMET Predictions.

Oxidative stress, the Warburg effect, and resistance to apoptosis are key hallmarks driving colorectal tumorigenesis. This study aimed to develop novel multi-target compounds capable of modulating these pathways. A library of 24 newly synthesized compounds-incorporating annulated thiophene, thiazole, quinazolinone, 2-oxoindoline, and 1,2,3-oxadiazole scaffolds, as well as -(1-(4-hydroxy-3-methoxyphenyl)-3-oxo-3-(2-(phenylcarbamothioyl)hydrazineyl) prop-1-en-2-yl)benzamide-was evaluated for antioxidant activity (DPPH assay), PDK-1 and LDHA inhibition, cytotoxic effects against LoVo and HCT-116 colon carcinoma cells, with parallel assessment of safety profiles on normal HUVECs.

Luteolin mitigates oxidative stress and multi-organ impairment in a propionic acid-induced rodent model of autism.

Background/objectives: Oxidative stress, organ impairments, and gastrointestinal abnormalities are the most common systemic dysfunctions that accompanied the neurodevelopmental condition, Autism Spectrum Disorder (ASD). Emerging evidence suggests that increased propionic acid (PPA) levels contribute to ASD pathophysiology through oxidative stress, neuroinflammation and disruption of the gut-liver-brain axis. Thanks to its strong anti-inflammatory and antioxidant potencies, luteolin, has shown to be promising in alleviating these effects.

Versatile biocatalyst: lipase from for ester synthesis and detergent innovation.

Background/objectives: The growing demand for reliable and stable biocatalysts has spurred research into microbial lipases for diverse industrial applications. This study focused on enhancing the production and purification of a lipase from (Lip).

Methods: Maximal lipase activity (420 U/mL) was achieved during the stationary phase after 84 h of incubation at 45°C and pH 8.

Comparative evaluation of certain biomarkers emphasizing abnormal GABA inhibitory effect and glutamate excitotoxicity in autism spectrum disorders.

Introduction: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social communication deficits and repetitive behaviors. An imbalance between the excitatory neurotransmitter glutamate and the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) might play a crucial role in ASD. This study explores the biochemical markers associated with GABAergic and glutamatergic signaling in individuals with autism and healthy controls, aiming to identify potential diagnostic and therapeutic targets.

Evaluating the antifungal potential of six essential oils against fungi causing wilt and dieback on olive trees.

Essential oils, known for their antimicrobial properties, are being investigated as natural alternatives to synthetic fungicides in agriculture. This study aimed to assess the chemical composition of six commercial essential oils (clove, tea tree, rosemary, thyme, oregano, and garlic) and to evaluate their fungistatic and/or fungicidal activity against six phytopathogenic fungi that cause significant damage to olive trees in Tunisia. For this purpose, the essential oils' qualitative and quantitative chemical compositions were analyzed using gas chromatography-mass spectrometry.

Exploring the antioxidant and enzyme inhibitory potential of the Streptomyces gobitri-cini strain: a promising biotechnological resource.

Streptomyces strains are renowned for their ability to produce a wide array of secondary metabolites, which play crucial roles in ecological interactions and have significant pharmaceutical applications. The optimization of culture conditions is a key factor in maximizing the production of these bioactive compounds. This study investigated the growth patterns and bioactivity of a newly isolated Streptomyces gobitricini cultured on different media: R5, R5E, R2YE, and YEME.

Unveiling the therapeutic potential: anti-inflammatory and antioxidant properties of selective medicinal plants.

This study highlights the potential of plant extracts as sustainable and cost-effective alternatives to traditional anti-inflammatory drugs, owing to their rich bioactive compounds. The chemical composition and biological activities of ethanolic extracts from Artemisia campestris, Haloxylon articulatum, and Retama raetam were investigated. Extraction yields ranged from 2.

Corrigendum: Unlocking the therapeutic potential of : purification and functional characterization of α-amylase inhibitors.

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Impact of GABA and nutritional supplements on neurochemical biomarkers in autism: a PPA rodent model study.

Background/objectives: Autism spectrum disorder (ASD) is associated with excitatory-inhibitory imbalance and oxidative stress. GABA, an inhibitory neurotransmitter, and related nutritional therapies are promising in restoring these imbalances. GABAergic deficits and glutamate excitotoxicity are two essential signaling pathways that could be addressed to treat autism, thus medications targeting these pathways are critical for treating behavioral symptoms.

Unlocking the therapeutic potential of : purification and functional characterization of α-amylase inhibitors.

Introduction: Regulating the catalytic activity of alpha-Amylase enzymes can decrease glucose production during the postprandial phase, potentially offering therapeutic benefits for diabetes. This research aimed to assess the inhibition of α-amylase using crude extracts from a medicinal plant traditionally used for treating diabetes and its associated complications.

Methods: Two novel potent proteinaceous amylase inhibitors: ScAI-R and ScAI-L were purified and characterized from roots and leaves.

Evaluation of the Combinatory Anticancer Effect of Chemotherapeutic Compounds and Prodigiosin against HCT-116, LoVo, and A549 Cell lines.

Despite their wide usage in reducing tumors and improving patients' survival, chemotherapeutic drugs or natural compounds are facing the development of cancer resistance. Many experimental data and clinical trials have shown that combinatorial treatment could be an efficient solution for some resistance problems. In this study, we aimed to evaluate the synergistic effects of combining prodigiosin (PG), a natural compound with known anticancer properties, with the commonly used chemotherapy drugs 5-fluorouracil (5-FU), oxaliplatin, and paclitaxel.

Nanotherapy for Cancer and Biological Activities of Green Synthesized AgNPs Using Aqueous Leaves and Roots Extracts.

: Nanoparticles derived from medicinal plants are gaining attention for their diverse biological activities and potential therapeutic applications. : This study explored the antioxidant, anti-inflammatory, anti-tumoral, and antimicrobial properties of green synthesized silver nanoparticles (AgNPs) using the aqueous leaf and root extracts of (). The physicochemical characterizations of both biosynthesized AgNPs using the aqueous leaf extract (L-AgNPs) and root extract (R-AgNPs) were examined using UV spectroscopy, fluorescence spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, dynamic light scattering, and Fourier-transform infrared spectroscopy.

Synthesis, molecular modelling, and biological evaluation of novel quinoxaline derivatives for treating type II diabetes.

Quinoxalines are benzopyrazine derivatives with significant therapeutic impact in the pharmaceutical industry. They proved to be useful against inflammation, bacterial, fungal, viral infection, diabetes and other applications. Very recently, in January 2024, the FDA approved new quinoxaline containing drug, erdafitinib for treatment of certain carcinomas.

Assessing the COX-2/PGE2 Ratio and Anti-Nucleosome Autoantibodies as Biomarkers of Autism Spectrum Disorders: Using Combined ROC Curves to Improve Diagnostic Values.

Autism spectrum disorder (ASD) is a neurodevelopmental condition marked by restricted and repetitive behaviors as well as difficulties with social interaction. Numerous studies have revealed aberrant lipid mediators and autoimmunity as a recognized etiological cause of ASD that is amenable to therapeutic intervention. In this study, the relationship between the relative cyclooxygenase-2/prostaglandin E2 ratio (COX-2/PGE2) as a lipid mediator marker and anti-nucleosome autoantibodies as an autoimmunity marker of ASD was investigated using multiple regression and combined receiver operating characteristic (ROC) curve analyses.

Combining Anti-Mitochondrial Antibodies, Anti-Histone, and PLA2/COX Biomarkers to Increase Their Diagnostic Accuracy for Autism Spectrum Disorders.

Background: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairments in social interaction and restricted and repetitive behaviors. Oxidative stress may be a critical link between mitochondrial dysfunction and ASD as reactive oxygen species (ROS) generated from pro-oxidant environmental toxicants and activated immune cells can result in mitochondrial failure. Recently, mitochondrial dysfunction, autoimmunity, and abnormal lipid mediators have been identified in multiple investigations as an acknowledged etiological mechanism of ASD that can be targeted for therapeutic intervention.

Effective Soybean Oil Degumming by Immobilized Phospholipases A from Venom.

Enzymatic degumming utilizing phospholipase enzymes could be used in ecologically friendly procedures with enhanced oil recovery yields. In this study, two phospholipases A of group I and II, WaPLA-I and WaPLA-II, from the snake venom of Saudi were evaluated for soybean oil degumming after being immobilized on three different support materials (calcium alginate (CA), CA-gelatin (CAG), and CA-chitosan (CAC), and cross-linked with glutaraldehyde). Higher yields of CAC-immobilized PLA-I (85 ± 3%) and PLA-II (87 ± 3.

Novel Stable Protease Inhibitor from Flowers with Antimicrobial and Antitumoral Activities.

A novel protease inhibitor isolated from date palm flowers (PIDF) was purified and characterized. A heat and acidic treatment step followed by ethanol precipitation and reverse-phase high-performance chromatography was applied to purify this natural protease inhibitor to homogeneity with a single band of about 19 kDa. The stability study depicted that PIDF was fully stable at 40 °C and retained 65% of its initial activity after heating at 50 °C for 24 h.

leaves extract mitigates cadmium induced hepatotoxicity via modulation of antioxidants, inflammatory markers and Nrf-2 signaling in mice.

Cadmium (Cd) is a harmful heavy metal that results in many toxic issues. showed potential pharmaceutical applications. This study investigated the possible ameliorative mechanism of leaves extract (UPLE) against hepatotoxicity induced by cadmium chloride (CdCl) in mice.

Involvement of Autophagy and Oxidative Stress-Mediated DNA Hypomethylation in Transgenerational Nephrotoxicity Induced in Rats by the Mycotoxin Fumonisin B1.

Fumonisin B1 (FB1), a mycotoxin produced by , is one of the most common pollutants in natural foods and agricultural crops. It can cause chronic and severe health issues in humans and animals. The aim of this study was to evaluate the transgenerational effects of FB1 exposure on the structure and function of the kidneys in offspring.

Synthesis and Biological Evaluation of Some New 3-Aryl-2-thioxo-2,3-dihydroquinazolin-4(1)-ones and 3-Aryl-2-(benzylthio)quinazolin-4(3)-ones as Antioxidants; COX-2, LDHA, α-Glucosidase and α-Amylase Inhibitors; and Anti-Colon Carcinoma and Apoptosis-Inducing Agents.

Oxidative stress, COX-2, LDHA and hyperglycemia are interlinked contributing pathways in the etiology, progression and metastasis of colon cancer. Additionally, dysregulated apoptosis in cells with genetic alternations leads to their progression in malignant transformation. Therefore, quinazolinones - and - were synthesized and evaluated as antioxidants, enzymes inhibitors and cytotoxic agents against LoVo and HCT-116 cells.

Functional Characterization and Anti-Tumor Effect of a Novel Group II Secreted Phospholipase A from Snake Venom of Saudi .

Secreted phospholipases A are snake-venom proteins with many biological activities, notably anti-tumor activity. Phospholipases from the same snake type but different geographical locations have shown similar biochemical and biological activities with minor differences in protein sequences. Thus, the discovery of a new phospholipase A with unique characteristics identified in a previously studied venom could suggest the origins of these differences.

Combined immobilized lipases for effective biodiesel production from spent coffee grounds.

This work describes the enzymatic transesterification of the oil extracted from SCGs for synthesis of biodiesel as a promising alternative to diesel fuels based on petroleum. Biocatalysts from various sources were tested for biodiesel synthesis using coffee oil among which CaCO- immobilized and showed the highest conversion yields (61 ± 2.64% and 64.

Unveiling sex-based differences in developing propionic acid-induced features in mice as a rodent model of ASD.

Background: Males are more likely to develop autism as a neurodevelopmental disorder than females are, although the mechanisms underlying male vulnerability are not fully understood. Therefore, studying the role of autism etiologies considering sex differences in the propionic acid (PPA) rodent model of autism would build greater understanding of how females are protected from autism spectrum disorder, which may be used as a treatment strategy for males with autism.

Objectives: This study aimed to investigate the sex differences in oxidative stress, glutamate excitotoxicity, neuroinflammation, and gut microbiota impairment as etiological mechanisms for many neurological diseases, with specific reference to autism.