Combined immobilized lipases for effective biodiesel production from spent coffee grounds.

This work describes the enzymatic transesterification of the oil extracted from SCGs for synthesis of biodiesel as a promising alternative to diesel fuels based on petroleum. Biocatalysts from various sources were tested for biodiesel synthesis using coffee oil among which CaCO- immobilized and showed the highest conversion yields (61 ± 2.64% and 64.