Brain-wide activation and deactivation maps during smooth and saccadic tracking in humans.

This study aimed to identify brain activity modulations associated with different types of visual tracking using advanced functional magnetic resonance imaging techniques developed by the Human Connectome Project (HCP) consortium. Magnetic resonance imaging data were collected from 27 healthy volunteers using a 3-T scanner. During a single run, participants either fixated on a stationary visual target (fixation block) or tracked a smoothly moving or jumping target (smooth or saccadic tracking blocks), alternating across blocks.

From neglected to notoriety: a review of Mpox clinical features, virology, epidemiology, treatment and prevention strategies.

Purpose: This narrative review aims to provide an overview of current knowledge on mpox, emphasizing updated epidemiology and recent advances in treatment and prevention strategies, in light of the latest outbreaks.

Methods: We searched PubMed and Google Scholar for publications on 'Mpox' and 'Monkeypox' up to June 5, 2025. Grey literature from governmental and health agencies was also accessed for outbreak reports and guidelines where published evidence was unavailable.

Influence of Cooking Methods on Phenolic Compounds and their Activities in Pea Shoots (Pisum sativum).

Pea shoots (Pisum sativum) are well known to have nutritional benefits when consumed raw; however, the effects of home cooking on their bioactive compounds remain unclear. Therefore, we investigated how different cooking methods affect the antioxidant activity and stability of antioxidants. Our evaluation revealed that antioxidant activity is preserved by steaming but significantly reduced by microwaving and boiling, which also causes weight loss during cooking.

Biological and Metabolomic Characterization of Human Dermal Fibroblasts and Mesenchymal Stem Cells Derived from Human Dental Pulp and Adipose Tissue: a Pilot Comparative Study.

Background: Several studies have suggested that adult human dermal fibroblasts (HDFa) may be a potential alternative source to mesenchymal stem cells for cell therapies. This study aims to characterize HDFa, adipose-derived stem cells (ADMSCs) and dental pulp stem cells (DPSCs) to investigate their proliferation, differentiation potential, mitochondrial respiration, and metabolomic profile. We identified molecules and characteristics that would differentiate MSCs from different sources or confirm their uniformity.

CT-Based Radiomics Models with External Validation for Prediction of Recurrence and Disease-Specific Mortality After Radical Surgery of Colorectal Liver Metastases.

Purpose: To build computed tomography (CT)-based radiomics models, with independent external validation, to predict recurrence and disease-specific mortality in patients with colorectal liver metastases (CRLM) who underwent liver resection.

Methods: 113 patients were included in this retrospective study: the internal training cohort comprised 66 patients, while the external validation cohort comprised 47. All patients underwent a CT study before surgery.

The predictive value of the orientation and offset of angle α and angle κ for visual outcomes after trifocal intraocular lens implantation in an Asian cohort.

Purpose: To evaluate the predictive value of the preoperative orientation and offset of angle alpha(chord alpha) and angle kappa(chord mu) for visual outcomes in patients who underwent trifocal intraocular lens (IOL) implantation.

Methods: Patient records of eyes that underwent AT LISA tri 839MP implantation were retrospectively collected and grouped according to the preoperative offset and orientations of chord alpha and chord mu. The two-dimensional location of each angle was described by the interaction of the orientation and offset.

Simple scoring model for predicting overt hepatic encephalopathy in geriatric cirrhosis: A multicenter retrospective cohort study.

Identifying the risk of overt hepatic encephalopathy (OHE) in geriatric patients with cirrhosis remains challenging. This study aimed to investigate the independent factors for OHE development in geriatric cirrhosis and to establish a simple scoring model to identify individuals at risk for OHE. We conducted a retrospective review of geriatric patients with cirrhosis aged ≥ 80 years who were admitted between April 2006 and November 2022.

Use of progestin-only drospirenone-based pills in hyperandrogenic women with polycystic ovary syndrome.

Purpose: Polycystic ovarian syndrome (PCOS) is a common endocrine-metabolic disorder affecting about 10% of reproductive-age women. Characterized by hyperandrogenism and ovulatory dysfunction, PCOS often involves metabolic features due to insulin resistance. Traditional treatment with combined oral contraceptive pills (COCP) effectively manages hyperandrogenism and menstrual irregularities.

Fulminant myocarditis: outcome predictors in an international cohort study.

Background And Aims: Fulminant myocarditis (FM) is a complex clinical syndrome characterized by acute myocardial inflammation and cardiogenic shock. Evidence on long-term outcomes, mortality risk factors, and targeted treatment options remains limited.

Methods: This retrospective analysis included consecutive adult patients admitted for FM between January 2012 and November 2022 at 26 European tertiary centres.

Genomic insights into multidrug-resistant extraintestinal pathogenic Escherichia coli O4: H12 harboring mcr-5 and blaSHV-12 on conjugative plasmids isolated from chicken meat in Japan.

Aims: This study aims to investigate the genomic profile of a multidrug-resistant Escherichia coli strain, 160-11H1, co-carrying an extended-spectrum β-lactamase (ESBL) and the plasmid-mediated mobile colistin resistance gene, mcr-5.

Methods And Results: The entire genome of the strain was sequenced using Illumina MiSeq and Oxford Nanopore platforms, and de novo assembly was performed using Unicycler. The genome size was 5 031,330 bp and comprised 5 140 coding sequences.

The role of health literacy in boosting citizen engagement in appropriate use of antibiotics.

Background: Antimicrobial resistance (AMR) represents a growing challenge to both individual and public health, driven by the excessive and inappropriate use of antibiotics. Studies emphasize a widespread lack of knowledge regarding proper antibiotic use and the mechanisms of antibiotic resistance. This study aims to explore the relationships between citizens' health engagement and attitudes towards antibiotic consumption (Hypothesis 1, Hypothesis 2) and explores the role of orientation to health literacy as a mediator of these relationships (Hypothesis 3).

Reframing Prehospital Termination of Resuscitation as Withdrawal of Life Support: Applying lessons from the ICU in the prehospital setting.

Cardiac arrest response and management is a critical piece of prehospital clinical practice yet the majority of these patients do not survive to be transported. Termination of resuscitation and resulting death notification is stressful and emotional for both loved ones and EMS clinicians. We describe a fundamental shift from traditional termination of resuscitation to a patient and family-centered model.

Two-coordinate Co and Ni complexes of a bulky imidazolin-2-iminato ligand and facile synthesis of heterobimetallic complexes.

We report the synthesis of homoleptic two-coordinate Co and Ni complexes supported by a sterically hindered NIm ligand (1,3-bis(2,6-diisopropylphenyl)imidazolin-2-iminato). Their formulation as two-coordinate complexes was verified by single-crystal X-ray diffraction analyses, and their M-N bond distances are between those in the reported transition-metal imido and amido complexes, suggesting a multiple-bond character. These coordinatively unsaturated complexes readily react with GeCl·dioxane, affording bimetallic Co/Ge and Ni/Ge complexes, demonstrating facile and rational syntheses of heterobimetallic complexes.

Spectral Photon-Counting Detector Coronary CTA With Reduced Radiation and Contrast Media Doses in Inflammation-Associated Coronary Artery Disease: A Prospective Study.

Patients with inflammation-associated coronary artery disease (CAD) may exhibit rapid progression and require regular coronary imaging. To evaluate the diagnostic performance of spectral photon-counting detector (PCD) coronary CTA with reduced radiation and contrast media doses for detecting coronary stenosis and in-stent restenosis in patients with inflammation-associated CAD. This prospective study enrolled patients with inflammation-associated CAD from January 2023 to March 2024.

Real-world treatment for systemic sclerosis and systemic sclerosis-associated interstitial lung disease: information from a Japanese hospital claims database.

ObjectivesThe 2023 EULAR guidelines for systemic sclerosis (SSc) newly recommend biologics (rituximab, tocilizumab), mycophenolate mofetil (MMF), and nintedanib in addition to cyclophosphamide for interstitial lung disease (ILD). This study investigated recent actual use of these drugs in Japan. MethodsWe analysed data from a Japanese hospital claims database (2020-2023), identifying patients with SSc disease codes (ICD-10 M34.

Feasibility and efficacy of exercise training on sleep symptoms and comorbidities in narcolepsy type 1: a prospective interventional study.

Current treatments for narcolepsy type 1 (NT1) have little impact on psychiatric, cognitive and metabolic comorbidities. Here, we evaluated the feasibility, safety and efficacy of a prospective Exercise Training (ET) program on sleep-related symptoms and comorbidities in NT1. Sedentary adult with NT1 participated in a 6-week supervised ET program followed by a 18-week self-directed program.

Elucidating the Structure and the Impact of Synthesis Methods on the Flexibility of the Metal-Organic Framework MIL-88 A (Fe) During Water Capture.

Flexible metal-organic frameworks (MOFs) have emerged as a new generation of porous materials and are considered for various applications such as sensing, water or gas capture, and water purification. MIL-88 A (Fe) is one of the earliest and most researched flexible MOFs, but to date, there is a lack in the structural aspects that govern its dynamic behaviour. Here, we report the first crystal structure of DMF-solvated MIL-88 A and investigate the impact of real structure effects on the dynamic behaviour of MIL-88 A (Fe), particularly upon water adsorption.

Plasma-Tunable Metasurfaces for SERS Nanosensing of Hazardous Chemicals.

The demand for rapid, field-deployable detection of hazardous substances has intensified the search for plasmonic sensors with both high sensitivity and fabrication simplicity. Conventional approaches to plasmonic substrates, however, often rely on lithographic precision or complex chemistries limiting scalability and reproducibility. Here, a facile, one-step synthesis of vertically aligned 2D nanosheets composed of intergrown CuO/CuO crystallites is presented, fabricated via oxygen plasma discharge on copper substrates.

Characterization of Fluorescent Reporters for Flow Cytometry-Based Single-Cell Studies in .

Fluorescent proteins (FPs) are commonly used as reporters to examine intracellular genetic, molecular, and biochemical status. Flow cytometry is a powerful technique for accurate quantification of single-cell fluorescent levels. Here, we characterize green, red, and blue FPs for use in yeast .

Modeling and Simulation Identifies Endocytosis Uptake Rate and Fraction Unbound as Important Predictors of Oligonucleotide Pharmacokinetics.

Therapeutic oligonucleotides (TOs) represent an emerging modality, which offers a promising alternative treatment option, particularly for intracellular targets. The two types of TOs, antisense oligonucleotides (ASO) and small interfering RNAs (siRNAs), distribute highly into tissues, especially into the liver and the kidneys. However, molecular processes at the cellular level such as the uptake into the cell, endosomal escape, binding to the target mRNA, and redistribution back to the systemic circulation are not well characterized because experimental data and assays are lacking.

Off-target structural insights: ArnA and AcrB in bacterial membrane-protein cryo-EM analysis.

Membrane-protein quality control in Escherichia coli involves coordinated actions of the AAA+ protease FtsH, the insertase YidC and the regulatory complex HflKC. These systems maintain proteostasis by facilitating membrane-protein insertion, folding and degradation. To gain structural insights into a putative complex formed by FtsH and YidC, we performed single-particle cryogenic electron microscopy on detergent-solubilized membrane samples, from which FtsH and YidC were purified using Ni-NTA affinity and size-exclusion chromatography.

A tetraphosphino-bis(-triazine) from cyanuric chloride and PhPH: new unexpected tandem C-C/C-P coupling reaction.

Serendipitous synthesis of 4,4',6,6'-tetrakis(diphenylphosphino)-2,2'-bi(-triazine) (Tr2P4) - a novel promising polyphosphine ligand - from cyanuric chloride and diphenylphosphine is reported. This unusual reaction represents the first example of tandem C-C/C-P coupling between (het)aryl halides and P-H species, yielding a structurally unique bi(hetaryl)-tetraphosphine. Theoretical study indicates that the 2,2'-bi(-triazine) core arises from hydrolysis of an intermediate [PhP(TrCl)] salt, formed through quaternization of monophosphine Tr(PPh)Cl with cyanuric chloride.

The Effectiveness and Profitability of Different Antithrombotic Therapy Regimens in Patients with Type 2 Diabetes Mellitus During 1 Year of Follow-Up after an Exacerbation of Coronary Heart Disease According to the Study ORACLE II.

Aim    To analyze the efficacy and cost-effectiveness of various options of antithrombotic therapy in patients with type 2 diabetes mellitus (T2DM) after acute coronary syndrome (ACS), based on the results of a one-year follow-up.Material and methods    The article presents features of various antithrombotic therapies in patients with T2DM after ACS from the standpoint of efficacy and cost-effectiveness in real clinical practice based on the materials of the ORACLE II open prospective observational study (2014-2017). The data of 368 patients were divided into three groups based on the selected antithrombotic therapy.