Impact of Serum/Xeno-Free Medium and Cytokine Supplementation on CAR-T Cell Therapy Manufacturing in Stirred Tank Bioreactors.

Chimeric antigen receptor T-cell (CAR-T) therapies have demonstrated clinical efficacy in treating haematological malignancies, resulting in multiple regulatory approvals. However, there is a need for robust manufacturing platforms and the use of GMP-aligned reagents to meet the clinical and commercial demands. This study investigates the impact of serum/xeno-free medium (SXFM) and cytokine supplementation on CAR-T cell production in static and agitated culture systems, using 24-well plate G-Rex vessels and 500 mL stirred tank bioreactors (STRs), respectively.

Overall Survival with Amivantamab-Lazertinib in -Mutated Advanced NSCLC.

Background: Previous results from this phase 3 trial showed that progression-free survival among participants with previously untreated (epidermal growth factor receptor)-mutated advanced non-small-cell lung cancer (NSCLC) was significantly improved with amivantamab-lazertinib as compared with osimertinib. Results of the protocol-specified final overall survival analysis in this trial have not been reported.

Methods: We randomly assigned, in a 2:2:1 ratio, participants with previously untreated -mutated (exon 19 deletion or L858R substitution), locally advanced or metastatic NSCLC to receive amivantamab-lazertinib, osimertinib, or lazertinib.

Epistasis at the cell surface: what is the role of Erg3 loss-of-function in acquired echinocandin resistance?

Echinocandins, which target the fungal β-1,3-glucan synthase (Fks), are essential for treating invasive fungal infections, yet resistance is increasingly reported. While resistance typically arises through mutations in Fks hotspots, emerging evidence suggests a contributing role of changes in membrane sterol composition due to mutations. Here, we present a clinical case of () in which combined mutations in and , but not alone, appear to confer echinocandin resistance.

Measuring the Science of Caring: A Patient-Centred Predictive Model for the Caring Interaction.

Background: The nurse-patient relationship is central to quality nursing care, yet its impact remains difficult to quantify. While existing models assess caring competencies from the perspective of nursing students and professionals, there is a lack of validated instruments incorporating direct patient feedback.

Objective: This study aimed to develop and validate the Nursing Interaction in Caring_Competence Assessment-Patient (NIC_CA-Patient) tool, a patient-centred instrument designed to measure caring interaction in nursing practice and establish a predictive model of its development from the patient's perspective.

EXPRESS: Influence of word Age-of-Acquisition (AoA), vocabulary size, formal-lexical similarity, and semantic richness of words on lexical recognition and production: A study on foreign-word training.

A distinctive feature of the lexicon is its susceptibility to the order in which words are acquired; those learned earlier are accessed and retrieved more quickly than those acquired later-a phenomenon known as the age of acquisition (AoA) effect. This study investigates how vocabulary size (i.e.

Implementation of a Feeding Protocol in Patients With Humidified HIGH Flow Nasal Cannula in a Pediatric Intensive Care Unit Improves Enteral Nutrition: The HIGHPE Study.

Purpose: There is limited evidence to guide the use of enteral nutrition (EN) for children with bronchiolitis who received Humidified high flow nasal cannula (HHFNC) and often kept nil per mouth for aspiration and progression to mechanical ventilation risk.

Methods: This quality improvement project included children with bronchiolitis who were supported by HHFNC in the paediatric intensive care unit (PICU). An algorithm to increase EN use in those participants was created by stakeholders.

Influence of Manufacturing Tolerance and Formula Thickness Type in the Prediction Error of Multifocal Intraocular Lens Power Calculation.

Purpose: To assess differences in intraocular lens (IOL) power calculation prediction error (PE) considering the manufacturing tolerance or exact power (EP) versus labeled power (LP), and to compare accuracy using the Barrett formula with optimized constant versus a thick-lens formula.

Methods: The PE and absolute PE were calculated for a random eye of patients implanted with the multifocal Liberty Q-Flex 640PM IOL (Medicontur Ltd) considering the LP and the EP provided by the manufacturer. The outcomes for the Barrett with optimized constant formula and a thick-lens formula personalized for the surgeon, biometer, and IOL were compared.

Evaluation of Perception of Photic Phenomena Through Diffractive Multifocal Intraocular Lens Designs by Its Virtual Implantation.

Purpose: To analyze the difference in objective and subjective photic phenomena following virtual implantation of three different presbyopia-correcting diffractive intraocular lens (IOL) designs.

Methods: The study was conducted at JENVIS Research Germany. A prospective cross-over and double-masked trial design was used.

Repurposed antihypertensive drugs for negative symptoms in schizophrenia: A systematic review and meta-analysis.

Schizophrenia is a complex psychiatric disorder characterized by positive, negative, and general psychopathological symptoms. While antipsychotic drugs are effective for positive symptoms, they provide limited benefit for negative symptoms, which are often persistent and strongly associated with functional disability. Additionally, up to 30% of patients exhibit resistance to current treatments, including clozapine.

Nail toxicities associated with cancer therapies: Insights and recommendations from the EADV task force dermatology for cancer patients.

Nail toxicities are a frequent yet underreported adverse effect of cancer therapies. These toxicities can significantly impact patients' quality of life and may lead to treatment modifications or interruptions. This narrative review aims to analyse the types, severity and underlying mechanisms of nail toxicities associated with cancer treatments as well as their management.

Expert consensus for prevention, diagnosis and management of persistent chemotherapy-induced alopecia.

Background: Persistent chemotherapy-induced alopecia (pCIA) is a distressing side effect of antineoplastic agents, imposing significant psychological burdens on cancer survivors. Despite its impact, there are no standardized guidelines for diagnosis, prevention or management.

Objective: To establish consensus-based definitions, diagnostic criteria, grading systems and management recommendations for pCIA.

From the Reclaimed Water Treatment Plant to Irrigation in Intensive Agriculture Farms: Assessment of the Fate of Antibiotics, Antibiotic Resistance Bacteria and Genes, and Microbial Pathogens at Real Scale.

This work aims to investigate the occurrence of 31 antibiotics (ABs), 2 bacteria ( and spp.) and their counterpart antibiotic-resistant bacteria (carbapenem and cephalosporin families), and several antibiotic-resistant genes (ARGs) throughout a full distribution system of reclaimed water (RW) in a real-scale scenario. The RW was analyzed (i) before and after the tertiary treatment (sand filtration and chlorination), (ii) during the storage period in secondary ponds before its use in irrigation, and (iii) directly in the droppers installed in four plastic-based greenhouses over 9 months.

Misalignment between the Directions of Propagation and Decay of Nanoscale-Confined Polaritons.

Anisotropic van der Waals crystals have gained significant attention in nano-optics and optoelectronics due to their unconventional optical properties, including anomalous reflection, canalization, and nanofocusing. Polaritons─light coupled to matter excitations─govern these effects, with their complex wavevector encoding key parameters such as wavelength, lifetime, field confinement, and propagation direction. However, determining the complex wavevector, particularly the misalignment between its real and imaginary parts, has remained a challenge due to the complexity of the dispersion relation.

Role of Liquid Composition in the Transient Liquid Assisted Growth of Superconducting YBaCuO Films.

The unparalleled loss-less electrical current conduction of high-temperature superconducting (HTS) materials encourages research on YBaCuO (YBCO) to unravel opportunities toward numerous applications. Nonetheless, production costs and throughput of the commercialized HTS Coated Conductors (CCs) are still limiting a worldwide spread. Transient liquid assisted growth (TLAG) is a non-equilibrium process displaying ultrafast growth rate which, when combined with chemical solution deposition (CSD), is emerging as a strong candidate to reduce the cost/performance ratio of YBCO superconductors.

Allergy to : Comprehensive review from the origin to the therapeutic approach.

is an ubiquitous mold commonly found in both outdoor and indoor environments. It is a common airborne mold recognized as a significant aeroallergen linked to pediatric allergic rhinitis and asthma. Although sensitization rates in children vary regionally, evidence suggests that allergy significantly impacts pediatric respiratory health and as its exposure worsens, respiratory outcomes in susceptible pediatric populations .

Real incidence and management of patients with suspected iodinated contrast media allergy in our area.

Introduction: Adverse reactions to iodinated contrast media (ICM) are very common due to its widespread use. Despite the fact that overall incidence of hypersensitivity reactions (HSRs) to ICM is low, the risk of severe outcomes needs a careful patient evaluation and management.

Methods: We conducted a retrospective epidemiological study that included patients referred to our Allergy Unit for suspected allergy to ICM in whom we carried out a protocolized allergic study based on skin and drug provocation tests (DPT).

The Relationship Between Child-to-Parent Violence, Adolescent Stressors, and Emotional Security: The Moderating Role of Parental Divorce.

Child-to-parent violence (CPV) is a problem of great social relevance, which maintains an exponential growth. The aim of this study was to examine the influence of adolescent stressors and emotional security in the family system as predictors of CPV (reactive and proactive), considering the moderating effect of parental divorce. A sample composed of 892 adolescents aged 14-18 years ( = 15.

Emergent Dirac fermions in graphene and underlying Au monolayer with two-dimensional ferrimagnetism.

Using angle-resolved photoemission spectroscopy (ARPES) with spin resolution, scanning tunneling microscopy/spectroscopy (STM/STS) and density functional theory (DFT) methods, we study the electronic structure of graphene-covered and bare Au/Co(0001) systems and reveal intriguing features, arising from the ferrimagnetic order in graphene and the underlying gold monolayer. In particular, a spin-polarized Dirac-cone-like state, intrinsically related to the induced magnetization of Au, was discovered at point. We have obtained a good agreement between experiment and theory for bare and graphene-covered Au/Co(0001) and have proven that both Au ferrimagnetism and the Dirac-cone-like band are intimately linked to the triangular loop dislocations present at the Au/Co interface.

How many (distinguishable) classes can we identify in single-particle analysis?

Heterogeneity in cryoEM is essential for capturing the structural variability of macromolecules, reflecting their functional states and biological significance. However, estimating heterogeneity remains challenging due to particle misclassification and algorithmic biases, which can lead to reconstructions that blend distinct conformations or fail to resolve subtle differences. Furthermore, the low signal-to-noise ratio inherent in cryo-EM data makes it nearly impossible to detect minute structural changes, as noise often obscures subtle variations in macromolecular projections.

The use of injectable orthobiologics for knee osteoarthritis: A formal ESSKA-ORBIT consensus. Part 2-Cell-based therapy.

Purpose: This European Society of Sports Traumatology, Knee Surgery and Arthroscopy (ESSKA) formal consensus aims to provide evidence- and expert opinion-based recommendations for the use of point-of-care- and expanded-cell-based therapy (CBT) in the treatment of knee osteoarthritis (OA), focusing on indications, preparation, and administration.

Methods: A multidisciplinary group of 77 leading experts in musculoskeletal regenerative medicine from 22 European Countries formed a steering group, a rating group, and a reader group. The steering group developed 23 questions, originating from 27 statements.

Phase II Dose-Randomized Study of Sunvozertinib in Platinum-Pretreated Non-Small Cell Lung Cancer With Epidermal Growth Factor Receptor Exon 20 Insertion Mutations (WU-KONG1B).

Purpose: WU-KONG1B (ClinicalTrials.gov identifier: NCT03974022) is a multinational phase II, dose-randomized study to assess the antitumor efficacy of sunvozertinib in pretreated patients with advanced non-small cell lung cancer (NSCLC) with epidermal growth factor receptor () exon 20 insertion mutations (exon20ins).

Methods: Eligible patients with advanced-stage exon20ins NSCLC were randomly assigned by 1:1 ratio to receive sunvozertinib 200 mg or 300 mg once daily (200 and 300 mg-rand cohorts).