Correction: Systematic bacteriophage selection for the lysis of multiple strains.

[This corrects the article DOI: 10.3389/fcimb.2025.

Interspecific competition with the American Xanthium orientale L. as a possible cause of the decline of the Old-World X. stumarium L.

Xanthium is represented in Europe by three species complexes: X. strumarium L., X.

tRNA hydroxylation is an epitranscriptomic modulator of metabolic states affecting Pseudomonas aeruginosa pathogenicity.

Post-transcriptional modification of transfer RNAs (tRNAs) represents an essential layer of translational regulation critical for bacterial adaptation to environmental changes. Increasing evidence links the tRNA epitranscriptome to pivotal roles in the regulation of gene expression and various cellular processes, including stress responses and establishment of virulence. In this study, we used mass spectrometry and nanopore sequencing to quantify and identify the sites of TrhPO-dependent tRNA hydroxylation in total and purified Pseudomonas aeruginosa tRNAs.

'What's in a name? Fit-for-purpose bacterial nomenclature': meeting report.

Rapid and economical DNA sequencing has resulted in a revolution in phylogenomics. The impact of changes in nomenclature can be perceived as an absolute necessity of scientific rigour, coupled with the slight inconvenience of needing to re-learn names. In relation to practical aspects of microbiology, for example, infectious disease diagnosis, there may, however, be potential dangers.

Genomic and phenotypic insights into the expanding phylogenetic landscape of the genus.

The fungal genus includes several life-threatening human pathogens as well as diverse saprobic species whose genome architecture, ecology, and evolutionary history remain less well characterized. Understanding how some lineages evolved into major pathogens remains a central challenge and may be advanced by comparisons with their nonpathogenic counterparts. Integrative approaches have become essential for delimiting species and reconstructing evolutionary relationships, particularly in lineages with cryptic diversity or extensive chromosomal rearrangements.

Complete genome of five phages.

tailed phages Bhz9, Bhz15, Bhz16, Bhz20, and Bhz21 were isolated from wastewater samples in Hamburg-Eppendorf (Germany). Their double-stranded DNA genomes range from 41,054 to 167,277 bp, encoding between 54 and 267 predicted genes, with a G + C content ranging from 36.4% to 48.

Gene sco1417 encodes a positive regulator of the de novo biosynthesis of pyridoxal 5'-phosphate (vitamin B6) in Streptomyces coelicolor M145.

Background: Actinomycetes of the genus Streptomyces are renowned for their highly developed and diverse specialized metaboliс pathways, and there is an extensive body of data on their specific and pleiotropic levels of regulation. Much less is known about routes leading to essential metabolites in this genus. In this work, we focused on elucidating the function of the highly conserved across Streptomyces gene SCO1417 for GntR type regulator in the model strain S.

a novel planctomycetotal strain from the volcanic habitat of Panarea divides by unusual lateral budding.

Unlabelled: Members of the phylum are ubiquitous bacteria that play important roles in the global carbon and nitrogen cycle. In this study, we sampled the shallow-sea hydrothermal vent system close to Panarea Island, Italy, and analyzed the bacterial diversity in this habitat using a cultivation-independent amplicon sequencing approach. Motivated by the observed abundance of members of the phylum , we employed cultivation conditions that facilitate the enrichment of planctomycetes and isolated strain Pan216.

Assessing coverage of the monitoring framework of the Kunming-Montreal Global Biodiversity Framework and opportunities to fill gaps.

The Kunming-Montreal Global Biodiversity Framework (GBF) is the most ambitious multilateral agreement on biodiversity to date. It calls for a whole-of-government and whole-of-society approach to halt and reverse biodiversity loss worldwide. The GBF's monitoring framework lays out how Parties to the Convention on Biological Diversity are expected to report on their progress.

Pan-carcinoma data driven analysis reveals platelet-activating factor receptor (PTAFR) involvement in immunosuppressive tumor microenvironment signaling and clinical prognosis.

Solid tumor carcinogenesis is driven by complex interactions within the tumor microenvironment (TME), which influence tumor initiation, progression, and response to treatment. The phospholipid platelet-activating factor (PAF) has been described to affect TME and growth; however, evidence so far has been limited to experimental in vitro and in vivo models. This study investigates the role of PAF receptor (PTAFR) across various carcinomas through patient data-driven analysis.

Systematic bacteriophage selection for the lysis of multiple strains.

is an opportunistic pathogen causing severe infections of the lung, burn wounds and eyes. Due to its intrinsic high antibiotic resistance the bacterium is difficult to eradicate. A promising therapeutic option is the use of -specific bacteriophages.

Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets.

Predicting prokaryotic phenotypes-observable traits that govern functionality, adaptability, and interactions-holds significant potential for fields such as biotechnology, environmental sciences, and evolutionary biology. In this study, we leverage machine learning to explore the relationship between prokaryotic genotypes and phenotypes. Utilizing the highly standardized datasets in the BacDive database, we model eight physiological properties based on protein family inventories, evaluate model performance using multiple metrics, and examine the biological implications of our predictions.

Draft genome sequence of DSM 15742 isolated from a patient's blood in Cleveland, Ohio.

This study describes the whole genome sequence of 2991-95 (DSM 15742) isolated from human blood in Cleveland, Ohio. The genome length is approximately 1.8 Mbp with 107 contigs and a G + C content of 37.

Adjunctive phage therapy improves antibiotic treatment of ventilator-associated-pneumonia with Pseudomonas aeruginosa.

Bacterial multidrug resistance poses an urgent challenge for the treatment of critically ill patients developing ventilator-associated pneumonia (VAP). Phage therapy, a potential alternative when conventional antibiotics fail, has been unsuccessful in first clinical trials when used alone. Whether combining antibiotics with phages may enhance effectiveness remains to be tested in experimental models.

An integrative re-evaluation of the species complex.

The species-rich species complex (FSAMSC; ) is well-known for including devastating plant pathogens and toxigenic species. However, this group of grass-loving fungi also accommodates soil saprobes, endophytes, mycoparasites and rare opportunistic pathogens of humans and other animals. Recent publications have highlighted the vast phylogenetic and biochemical diversity of the FSAMSC, although a large number of taxa in FSAMSC have not been systematically described and still lack Latin binomials.

HiBC: a publicly available collection of bacterial strains isolated from the human gut.

Numerous bacteria in the human gut microbiome remain unknown and/or have yet to be cultured. While collections of human gut bacteria have been published, few strains are accessible to the scientific community. We have therefore created a publicly available collection of bacterial strains isolated from the human gut.

Genetic databases in the era of 'DSI' benefit-sharing.

Genetic databases drive research by enabling open access. Recently, parties to the Convention on Biological Diversity agreed on new rules for sharing benefits from the use of digital sequence information (DSI) which upholds open access, and also imposed new requirements for data depositors, database managers, and users.

Expanding the cultivable human archaeome: sp. nov. and strain 'GRAZ-2' from human faeces.

Two mesophilic, hydrogenotrophic methanogens, WWM1085 and GRAZ-2, were isolated from human faecal samples. WWM1085 was isolated from an individual in the United States and represents a novel species within the genus GRAZ-2 (=DSM 116045) was retrieved from a faecal sample of a European, healthy woman and represents a novel strain within this species. Both representatives form non-flagellated, short rods with variable morphologies and the capacity to form filaments.

Polyfloral nutritional resources promote bumble bee colony development after exposure to a pesticide mixture.

Bumble bees are important pollinators of crops in the field and greenhouses. They are naturally exposed to a combination of interacting stressors, e.g.

Phage-phage competition and biofilms affect interactions between two virulent bacteriophages and Pseudomonas aeruginosa.

Virulent bacteriophages (or phages) are viruses that specifically infect and lyse a bacterial host. When multiple phages co-infect a bacterial host, the extent of lysis and dynamics of bacteria-phage and phage-phage interactions are expected to vary. The objective of this study is to identify the factors influencing the interaction of two virulent phages with different Pseudomonas aeruginosa growth states (planktonic, an infected epithelial cell line, and biofilm) by measuring the bacterial time-kill and individual phage replication kinetics.

Navigating COP16's digital sequence information outcomes: What researchers need to do in practice.

The UN Convention on Biological Diversity adopted new rules for sharing benefits from publicly available genetic sequence data, also known as digital sequence information (DSI). In this Opinion, the authors describe the key elements researchers need to be aware of, address real-life questions, and explain the practical implications of these rules for research and development.

Tissue-like multicellular development triggered by mechanical compression in archaea.

The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal multicellularity in haloarchaea, forming tissue-like structures. These archaeal tissues are mechanically and molecularly distinct from their unicellular lifestyle, mimicking several eukaryotic features.