Role of extensification strategies in chicken meat quality.

The latest studies suggest a beneficial influence of husbandry factors, including increased space allowance, access to perches, and roughage, on the welfare of chickens; however, their effects on meat quality are divergent. Two experiments (EXP1 and EXP2) were performed to determine the husbandry factors affecting the meat quality of 42-day-old Ross 308 chickens (n = 180). The examined factors in EXP1 were stocking density (35 kg/m2 vs 41 kg/m2) and enrichment (access to perches), whereas in EXP2, enrichment (perches) and access to roughage (dried lucerne) were examined.

Nfe2l1 dysfunction alters Parkinson's disease-related gene expression and impairs neuronal differentiation under ubiquitin stress in neuronal differentiated P19 Cells.

Proteostasis is essential for neuronal health, and its disruption is implicated in neurodegenerative diseases such as Parkinson's disease (PD). Nfe2l1, a key regulator of proteostasis and ubiquitination, plays a significant role in neuronal health, yet its molecular functions in neuronal cells remain unclear. Our study investigates the role of Nfe2l1 in RA-induced neuronal differentiation of P19 cells under proteasome inhibition.

Phenotypic and Genotypic Evaluation of the Bitter Taste Receptor T2R38 in Chronic Rhinosinusitis.

<b>Introduction:</b> Chronic rhinosinusitis (CRS) is a multifactorial disease in which genetic predisposition plays a significant role. The bitter taste receptor T2R38, expressed in nasal and sinus mucosa, supports innate immunity. Single nucleotide polymorphisms (SNPs) in the <i>TAS2R38</i> gene affect individuals' ability to perceive its agonist - phenylthiocarbamide (PTC) - as bitter.

A 360° perspective on cardiovascular prevention: the International Lipid Expert Panel SiMple tIps for the heaLthy hEart (ILEP-SMILE).

Cardiovascular disease (CVD), particularly atherosclerotic cardiovascular disease (ASCVD), is the most common cause of death worldwide. Despite decades of research into lifestyle factors and medications, we still cannot effectively prevent the occurrence of ASCVD or the many incident CVD events and their complications. Yet, in most countries, only a small percentage of the healthcare budget is allocated to (primary) prevention, and only a few nations have introduced effective integrated (coordinated) programmes in primary prevention to promote health education, invest in health as early as possible, and prevent adverse CVD events.

Exploring #HealthLiteracy on LinkedIn: a first look at health hashtag engagement in a professional social media context.

LinkedIn, despite its large and professionally credentialed user base, remains an underexplored platform for digital health communication, unlike X (formerly Twitter), which has been widely studied for health-related hashtag trends. Health literacy, a key determinant of public health, is increasingly promoted through social media hashtags such as #HealthLiteracy. However, to date, no studies have systematically examined how this hashtag is used on LinkedIn.

Characterising nosing behaviours in pigs after mixing using social network analysis.

Affiliation as indicated by proximity has, to date, been used as the principal measure of positive relationships in farm animals. However, intensive housing may present a caveat to using proximity as a representation of affiliation as animals may be forced into proximity by stocking densities. To investigate affiliation patterns, this study examined differences in the expression of proximity and contact behaviours following regrouping in pigs.

Current Approaches to Aflatoxin B1 Control in Food and Feed Safety: Detection, Inhibition, and Mitigation.

Aflatoxins, toxic secondary metabolites produced primarily by and , pose a significant global health concern due to their frequent presence in crops, food, and feed-especially under climate change conditions. This review addresses the growing threat of aflatoxins by analyzing recent advances in detection and mitigation. A comprehensive literature review was conducted, focusing on bioremediation, physical and chemical detoxification, and fungal growth inhibition strategies.

Ginger () dietary supplementation in mice regulates liver antioxidant defense systems in a dose- and age-dependent.

Introduction: Oxidative stress and impaired antioxidant defenses contribute significantly to liver dysfunction, particularly with aging. This study evaluated the dose- and age-dependent effects of dietary ginger () supplementation on liver antioxidant defense systems in mice.

Methods: Male Swiss Webster mice aged 3, 6, and 12 months (n = 48 per age group) received standard feed or feed supplemented with either 0.

Cell-context response to germ layer differentiation signals is predetermined by the epigenome in regionalized epiblast populations.

Stem cells hold promise in regenerative medicine as they have the potential to differentiate into a variety of specialized cell types. However, mechanisms underlying stem cell potency and lineage acquisition remain elusive. Epigenetic modifications and genome accessibility prime cellular feedback to signalling cues, influencing lineage differentiation outcomes.

Biomarkers of Calcification, Endothelial Injury, and Platelet-Endothelial Interaction in Patients with Aortic Valve Stenosis.

Aortic stenosis (AS) is a progressive valvular heart disease characterized by fibrocalcific remodeling, inflammation, and hemodynamic disturbances. Serum biomarkers may indirectly reflect these processes. Autotaxin (ATX) and lysophosphatidic acid (LPA) have been implicated in osteogenic differentiation of valvular interstitial cells, while growth differentiation factor-15 (GDF-15) reflects cellular stress and vascular changes.

NFE2L1 as a central regulator of proteostasis in neurodegenerative diseases: interplay with autophagy, ferroptosis, and the proteasome.

Maintaining proteostasis is critical for neuronal health, with its disruption underpinning the progression of neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's diseases. Nuclear Factor Erythroid 2-Related Factor 1 (NFE2L1) has emerged as a key regulator of proteostasis, integrating proteasome function, autophagy, and ferroptosis to counteract oxidative stress and protein misfolding. This review synthesizes current knowledge on the role of NFE2L1 in maintaining neuronal homeostasis, focusing on its mechanisms for mitigating proteotoxic stress and supporting cellular health, offering protection against neurodegeneration.

Butyrate Derivatives Exhibited Anti-Inflammatory Effects and Enhanced Intestinal Barrier Integrity in Porcine Cell Culture Models.

Butyrate and its derivatives may influence inflammatory status and physiology in a variety of organisms and organ systems. Inflammatory conditions of the gastrointestinal tract, such as post-weaning diarrhea, negatively impact swine. Dietary intervention with butyrate-based compounds should be considered a strategy to improve disease resistance in pigs.

Breathing together: A global hashtag analysis of #LungHealth on platform X (formerly Twitter).

Background: The X platform has gained popularity in healthcare, with posts among physicians increasing by 112% over five years. In the context of pulmonology, #LungHealth is used for engagement, spreading awareness, and disseminating information on respiratory diseases, yet its impact remains unexplored.

Objective: We aim to analyze #LungHealth by measuring outreach and quantitative engagement metrics associated with #LungHealth.

Seaweed-derived mixed extracts exhibit immunomodulatory properties on porcine alveolar macrophages.

Antimicrobial resistance is a growing global concern, prompting for antibiotic alternatives in animal production. Seaweed, abundant in bioactive compounds with anti-inflammatory properties, offers a natural substitute to synthetic compounds. Considering this, the objective of the present study was to evaluate the anti-inflammatory bioactivity of three seaweeds 1:1 combination of Ascophyllum nodosum, Palmaria palmata, and Ulva lactuca.

Oxysterols, age-related-diseases and nutritherapy: Focus on 7-ketocholesterol and 7β-hydroxycholesterol.

Age-related diseases are often associated with a disruption of RedOx balance that can lead to lipid peroxidation with the formation of oxysterols, especially those oxidized on carbon-7: 7-ketocholesterol (also known as 7-oxo-cholesterol) and 7β-hydroxycholesterol. Like cholesterol, these oxysterols have 27 carbons, they are composed of a sterane nucleus and have a hydroxyl function in position 3. The oxysterols 7-ketocholesterol and 7β-hydroxycholesterol are mainly formed by cholesterol autoxidation and are biomarkers of oxidative stress.

Effects of husbandry practices on Ross 308 and Hubbard chicken hybrids welfare: HUSBANDRY EFFECTS ON BROILER WELFARE.

This study evaluated the impact of husbandry practices including enrichments, diet, stocking density, genotype, and outdoor access on welfare indicators in fast-growing Ross 308 and two genotypes of medium-growing Hubbard hybrids of chickens. The set of studies was designed with specific factors relevant to each genotype, with stocking density, perch enrichment and lucerne provision targeted for Ross 308 broilers and perch, outdoor access, as well as genotype (JA757 and JA787) for Hubbard chickens. Feather condition, footpad dermatitis (FPD), comb wounds, skin injuries, hock burn, lameness, dirtiness, toe damage, respiratory infections, and diarrhea were evaluated at the end of each production cycle.

Online Information About Side Effects and Safety Concerns of Semaglutide: Mixed Methods Study of YouTube Videos.

Background: Social media has been extensively used by the public to seek information and share views on health issues. Recently, the proper and off-label use of semaglutide drugs for weight loss has attracted huge media attention and led to temporary supply shortages.

Objective: The aim of this study was to perform a content analysis on English YouTube (Google) videos related to semaglutide.

Application of curcuminoids in inflammatory, neurodegenerative and aging conditions - Pharmacological potential and bioengineering approaches to improve efficiency.

Curcumin, a natural compound found in turmeric, has shown promise in treating brain-related diseases and conditions associated with aging. Curcumin has shown multiple anti-inflammatory and brain-protective effects, but its clinical use is limited by challenges like poor absorption, specificity and delivery to the right tissues. A range of contemporary approaches at the intersection with bioengineering and systems biology are being explored to address these challenges.

Predicting Post-surgery Discharge Time in Pediatric Patients Using Machine Learning.

Background: Prolonged hospital stays after pediatric surgeries, such as tonsillectomy and adenoidectomy, pose significant concerns regarding cost and patient care. Dissecting the determinants of extended hospitalization is crucial for optimizing postoperative care and resource allocation.

Objective: This study aims to utilize machine learning (ML) techniques to predict post-surgery discharge times in pediatric patients and identify key variables influencing hospital stays.

Fertility preservation of vacuum-dried ram spermatozoa stored for four years at room temperature.

Dry storage at room temperature (RT) could simplify spermatozoa banking. Here, we explored DNA stability and in vitro and in vivo development of embryos derived from vacuum-dried encapsulated (VDE) ram spermatozoa stored for four years or after accelerated aging. While some genomic damage was detected at time 0, DNA fragmentation increased from 3.

Advances in induced anhydrobiosis for cell and gamete storage.

It will appear strange to most readers, but lyophilization of spermatozoa was described by Chris Polge's first article that demonstrated the viability of spermatozoa stored in liquid nitrogen (LN. Since then, cryopreservation has become the only biobanking choice. The demonstration that lyophilized spermatozoa remain fertile has rekindled interest in noncryogenic biobanking.

A method for siRNA-mediated knockdown of target genes in RA-induced neurogenesis using P19 cells.

This study presents a comprehensive protocol for siRNA-mediated knockdown in the differentiation of P19 cells into neuronal-like cells. Utilizing a retinoic acid (RA)-induced neurogenesis model, P19 cells were cultured under specific conditions that facilitated the formation of embryoid bodies (EBs), which were subsequently differentiated into neuronal-like cells. In this investigation, we specifically targeted the Nfe2l1 gene using siRNA transfection to assess the efficiency and effectiveness of our protocol throughout the neuronal differentiation process.

Iron status and risk of iron disorders in neonates: A narrative review of recent studies in animal models.

The iron regulation mechanisms are not exactly the same between adulthood and the early postnatal period. Also, neonatal iron status is different in full-term versus preterm infants because the prenatal/gestational period, when hepatic iron accumulates, is shortened. Newborns, especially premature infants, are at high risk of iron deficiency due to inadequate iron stores, which constitute the primary source of iron to satisfy the neonate's increasing iron requirements.

Bridging Traditions and Technology: The Role of Ethnopharmacology in Shaping Next-Generation Multidisciplinary Researchers.

of the key disciplines that equip next-generation researchers engaged in ethnopharmacology research with the necessary knowledge and skills to navigate the transition from traditional ethnopharmacology to modern drug discovery.

Impact of #PCOSweightloss: A global X hashtag analysis study of weight loss narratives in the PCOS community.

Introduction: Social media plays a significant role in fostering communities around health and wellness. The hashtag #PCOSweightloss has become a pivotal forum on the platform X, where individuals exchange experiences, share information, and motivate each other concerning weight loss strategies connected with Polycystic Ovary Syndrome (PCOS).

Objective: This study aims to analyze the trends of #PCOSweightloss on X to gain insights into the social media metrics, including overall hashtag reach, key themes, and potential influence on management practices of PCOS.