Indication for biologics in a real-world cohort of dupilumab treated chronic rhinosinusitis with nasal polyps patients according to international recommendations: evidence from the European CRS Outcome Registry (CHRINOSOR).

Background: Criteria for biologic treatment of uncontrolled severe chronic rhinosinusitis with nasal polyps (CRSwNP) differ across international recommendations and prescription of biologics depends on national reimbursement criteria. CHRINOSOR offers an opportunity to analyse biologic indications in the real-world setting according to international recommendations.

Methods: CRSwNP patients who received dupilumab treatment in the ENT clinic of 6 tertiary centres (5 countries) were included.

Epidemiology, pathophysiology, diagnosis and management of atrial functional mitral regurgitation: An expert opinion paper.

Atrial functional mitral regurgitation (AFMR) is an increasingly recognized subtype of mitral regurgitation, characterized by left atrial remodelling and mitral annular dilation in the absence of primary mitral valve disease or left ventricular dysfunction. Closely linked to chronic atrial fibrillation and heart failure with preserved ejection fraction, AFMR is associated with poor clinical outcomes and represents a growing therapeutic challenge. This expert opinion paper summarizes current evidence on the epidemiology, pathophysiology, diagnosis and management strategies, including medical therapy and emerging data supporting surgical and transcatheter interventions in selected patients.

Clinical Characteristics and Disease Burden of Wheat Allergy Dependent on Augmentation Factors in Recreationally Active and Trained Individuals.

In wheat allergy dependent on augmentation factors (WALDA), allergic reactions occur when wheat ingestion is combined with exercise or rarely other augmentation factors. We analyzed clinical characteristics and disease burden in recreationally active and trained individuals with WALDA diagnosed by oral challenge test. Clinical characteristics, serological data, and quality of life (QOL) questionnaires were analyzed and completed with follow-up interviews.

Solid-state polycyclotrimerization of diynes to porous organic polymers.

Herein, we report a solid-state polycyclotrimerization of 1,4-diethynylbenzene using mechanochemical activation in a ball mill, yielding a highly porous and hydrophobic hyperbranched polymer (HBP) with a specific surface area of up to 570 m g. The reaction, catalyzed by Fe(hmds) and conducted under solvent-free conditions, was optimized by varying milling time and frequency. This method enables the efficient synthesis of insoluble, porous organic polymers with high yields (up to 95%) and offers an environmentally friendly alternative to traditional solution-based polymerizations.

Medical treatment of fibroids: FIGO best practice guidance.

Even though uterine fibroids are a widespread condition, the range of approved medical treatment options remains limited. In fact, only a few drugs are officially approved for the therapy of fibroids. In both the USA and the European Medicines Agency region, selected gonadotropin-releasing hormone (GnRH) antagonists have been approved for this indication.

Biofilm Analysis by Confocal Microscopy-Basics and Practical Aspects.

This review is intended as a guideline for beginners in confocal laser scanning microscopy. It combines basic theoretical concepts, such as fluorescence principles, resolution limits, and imaging parameters with practical guidance on sample preparation, staining strategies, and data acquisition using confocal microscopy. The aim is to combine technical and methodological aspects in order to provide a comprehensive and accessible introduction.

Multiyear Drought Strengthens Positive and Negative Functional Diversity Effects on Tree Growth Response.

Mixed-species forests are proposed to enhance tree resistance and resilience to drought. However, growing evidence shows that tree species richness does not consistently improve tree growth responses to drought. The underlying mechanisms remain uncertain, especially under unprecedented multiyear droughts.

BOLD Long-Range Temporal Correlations Reflect Changes in Language and Depression Across Intensive Aphasia Therapy.

Background: Intensive language-action therapy treats language deficits and depressive symptoms in chronic poststroke aphasia, yet the underlying neural mechanisms remain underexplored. Long-range temporal correlations (LRTCs) in blood oxygenation level-dependent signals indicate persistence in brain activity patterns and may relate to learning and levels of depression. This observational study investigates blood oxygenation level-dependent LRTC changes alongside therapy-induced language and mood improvements in perisylvian and domain-general brain areas.

Oxidized high-density lipoprotein associates with cardiometabolic dysfunction in coronary artery disease and acute coronary syndrome.

Background: High-density lipoprotein (HDL) function, rather than its concentration, plays a crucial role in the development of coronary artery disease (CAD). Diminished HDL antioxidant properties, indicated by elevated oxidized HDL (nHDL) and diminished paraoxonase-1 (PON-1) activity, may contribute to vascular dysfunction and inflammation. Data on these associations in CAD patients, including acute coronary syndrome (ACS), remain limited.

Similar Outcome With Haploidentical, Matched Sibling, or Matched Unrelated Donor Hematopoietic Cell Transplantation for Adult Patients With Adverse-Risk TP53-Mutated Acute Myeloid Leukemia in First Remission: A Comparative Study From the Global Committee and the Acute Leukemia Working Party of the European Society for Blood and Marrow Transplantation.

Given the dismal prognosis for patients with TP53-mutated acute myeloid leukemia (AML), the optimal donor for those undergoing allogeneic hematopoietic cell transplantation (allo-HCT) remains unclear. We retrospectively analyzed adult patients with TP53-mutated AML who underwent first allo-HCT in CR1 between 2010 and 2021. Outcomes were compared among using a haploidentical donor (Haplo), a matched sibling donor (MSD), and a 10/10 matched unrelated donor (MUD).

The burden of dengue fever in travellers: a systematic literature review.

Background: Dengue is a mosquito-borne viral infection with growing global impact, including international travellers travelling to and from endemic regions. This systematic literature review aimed to assess the clinical and economic burden of dengue in travellers from non-endemic countries.

Methods: This systematic review was conducted following the PRISMA guidelines to assess the incidence, prevalence, mortality, healthcare resource use, and costs of dengue fever in travellers between non-endemic and endemic regions.

A myotropic AAV vector combined with skeletal muscle -regulatory elements improve glycogen clearance in mouse models of Pompe disease.

Pompe disease is a glycogen storage disorder caused by mutations in the acid α-glucosidase (GAA) gene, leading to reduced GAA activity and glycogen accumulation in heart and skeletal muscles. Enzyme replacement therapy with recombinant GAA, the standard of care for Pompe disease, is limited by poor skeletal muscle distribution and immune responses after repeated administrations. The expression of GAA in muscle with adeno-associated virus (AAV) vectors has shown limitations, mainly the low targeting efficiency and immune responses to the transgene.

Case Report: Acute polymorphic psychosis and NMDA-R IgG antibodies in serum: a follow-up case study.

Introduction: Anti-N-methyl-D-aspartate receptor (NMDA-R) encephalitis is a neuropsychiatric disorder with additional psychiatric features caused by NMDA-R immunoglobulin G (IgG) antibodies in cerebrospinal fluid (CSF). This report presents the follow-up of a patient in whom we assumed mild NMDA-R encephalitis in the first psychotic episode.

Case Study: A patient with a prior episode of an acute polymorphic psychotic syndrome relapsed five and a half years later following a severe COVID-19 infection.

Integrative gene and isoform co-expression networks reveal regulatory rewiring in stress-related psychiatric disorders.

Isoform-specific expression patterns have been linked to stress-related psychiatric disorders such as major depressive disorder (MDD). To further explore their involvement, we constructed co-expression networks using total gene expression (TE) and isoform ratio (IR) data from affected ( = 210, 81% with depressive symptoms) and unaffected ( = 95) individuals. Networks were validated using advanced graph generation methods.

Exploring the biomechanical complexity of glioblastoma spheroids and organoids with co-localized Brillouin and Raman microspectroscopy.

Brillouin microscopy allows mechanical investigations of biological materials at the subcellular level and can be integrated with Raman spectroscopy for simultaneous chemical mapping, thus enabling a more comprehensive interpretation of biomechanics. The present study investigates different in vitro glioblastoma models using a combination of Brillouin and Raman microspectroscopy. Spheroids of the U87-MG cell line and two patient-derived cell lines as well as patient-derived organoids were used.

Optimizing Workflow for Cone Beam Computed Tomography-Based Online Adaptive Radiation Therapy Toward Reduced Physician Involvement.

Purpose: To evaluate the impact of an optimized online adaptive radiation therapy workflow on physician involvement.

Methods And Materials: Data from a prospective phase 2 trial involving 34 prostate cancer patients treated with cone beam computed tomography (CBCT)-based online adaptive radiation therapy (62 Gy in 20 fractions) were analyzed. Manual interventions were required for 2 steps in the workflow: radiation therapy technologist review and adjustment of automatically segmented organs, guiding target segmentation, so-called "influencer," while physicians reviewed and refined the targets.

Unravelling the molecular network structure of biohybrid hydrogels.

Glycosaminoglycan-based biohybrid hydrogels represent a powerful class of cell-instructive materials with proven potential in tissue engineering and regenerative medicine. Their biomedical functionality relies on a nanoscale polymer network that standard microscopy techniques cannot resolve. Here, we introduce an advanced analytical approach that integrates transmission electron microscopy, X-ray scattering, and computer simulations to directly and quantitatively characterize the nanoscale molecular network structure of these hydrogels.

Influence of consumer socio-psychological food environment on food choice and its implications for nutrition: evidence from Tanzania.

Background: Changes in consumer food choices have been associated with transformation in the food environment. Despite the direct impact of consumers' food choices on their diet and health outcomes, there is a lack of comprehensive evidence regarding how various factors within the food environment impact these choices.

Methods: This study uses the Theory of Planned Behavior to examine how socio-psychological factors in the food environment influence consumers' healthy food choices.

AI Algorithm for Lung Adenocarcinoma Pattern Quantification (PATQUANT): International Validation and Advanced Risk Stratification Superior to Conventional Grading.

The morphological patterns of lung adenocarcinoma (LUAD) are recognized for their prognostic significance, with ongoing debate regarding the optimal grading strategy. This study aimed to develop a clinical-grade, fully quantitative, and automated tool for pattern classification/quantification (PATQUANT), to evaluate existing grading strategies, and determine the optimal grading system. PATQUANT was trained on a high-quality dataset, manually annotated by expert pathologists.

Is -Associated Hearing Loss (DFNB93) a Gene Therapy Target? Preclinical Progress and Patient Registry.

modulates presynaptic Ca1.3 Ca channel function in inner hair cells (IHCs) and is required for indefatigable synaptic sound encoding. Biallelic variants in are associated with non-syndromic hearing loss (DFNB93).

Enhancing diagnostic precision: a multicentric study of endobronchial ultrasound-guided transbronchial mediastinal cryobiopsy in lymphoproliferative disorders.

Background: The diagnostic accuracy of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) for the diagnosis of lymphoma has a low overall sensitivity. The lack of tissue architecture obtained by cytological needles decreases the diagnostic accuracy for diagnosis and subtyping of and relapsed mediastinal lymphomas. This study compares the sensitivity of EBUS-TBNA and endobronchial ultrasound-guided transbronchial mediastinal cryobiopsy (EBUS-TMC) for both initial lymphoma diagnosis and recurrent cases, analyses safety, and evaluates the number of mediastinoscopies that could potentially be avoided due to this novel technique.

Radiomics features from the peritumoral region can be associated with the epilepsy status of glioblastoma patients.

Purpose: Identifying radiomics features that help predict whether glioblastoma patients are prone to developing epilepsy may contribute to an improvement of preventive treatment and a better understanding of the underlying pathophysiology.

Materials And Methods: In this retrospective study, 3-T MRI data of 451 pretreatment glioblastoma patients (mean age: 61.2 ± 11.

High-resolution imaging system for integration into intelligent noncontact total body scanner.

Significance: Melanoma's rising incidence demands automatable high-throughput approaches for early detection such as total body scanners, integrated with computer-aided diagnosis. High-quality input data is necessary to improve diagnostic accuracy and reliability.

Aim: This work aims to develop a high-resolution optical skin imaging module and the software for acquiring and processing raw image data into high-resolution dermoscopic images using a focus stacking approach.

Phasor and neural network approaches for rapid fluorophore fraction analysis in temporal-spectral multiplexed data.

Significance: The spatial and temporal distribution of fluorophore fractions in biological and environmental systems contains valuable information about the interactions and dynamics of these systems. To access this information, fluorophore fractions are commonly determined by means of their fluorescence emission spectrum (ES) or lifetime (LT). Combining both dimensions in temporal-spectral multiplexed data enables more accurate fraction determination while requiring advanced and fast analysis methods to handle the increased data complexity and size.

Management of a complex central bile duct injury associated with laparoscopic cholecystectomy: a case report of double hepaticojejunostomy and advanced transhepatic interventional drainage technique.

We describe a case of a 64-year-old obese woman with a history of severe acute cholecystitis and choledocholithias who underwent laparoscopic cholecystectomy in our clinic after endoscopic treatment by sphincterotomy and stent insertion. On the first operative day, a significant bile leakage of 400 ml appeared in the drainage. An immediate surgical revision was performed, starting by laparoscopy with conversion to open surgery.