Seed Polyphenols Protect RAW264.7 Macrophages by Inhibiting Oxidative Stress and Inflammation.

The seeds of are rich in polyphenols, yet their chemical characteristics and biological activities require systematic elucidation. In this study, seed polyphenols (TGSP) were prepared using ultrasound-assisted extraction (70% ethanol, solid-to-liquid ratio of 1:40 g/mL, 210 W power, 55°C, 50 min) coupled with AB-8 macroporous resin purification. The resulting TGSP were characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared (FT-IR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS).