4 results match your criteria: "China. Electronic address: gyliu@shxh-centerlab.com.[Affiliation]"
A novel automated multi-cycle magnetic solid-phase extraction coupled to LC-MS/MS to study the disorders of six functional B vitamins in patients with gastroenterology and hyperhomocysteinemia.
J Pharm Biomed Anal
April 2024
Central Laboratory, Shanghai Xuhui Central Hospital/Zhongshan-Xuhui Hospital, Fudan University, Shanghai 200031, PR China; Shanghai Engineering Research Center of Phase I Clinical Research & Quality Consistency Evaluation for Drugs, Shanghai 200031, PR China; Institute of Clinical Mass Spectrometry,
B vitamins are essential for human life and their disorders can cause a variety of diseases. Solid-phase extraction (SPE) coupled to LC-MS/MS is a preferred technique for determining multiple B vitamins, however, their complexity in real biological matrices makes it hard to achieve satisfactory recovery and accuracy when simultaneous detection. In this study, a novel automated multi-cycle magnetic SPE (MSPE) coupled to the LC-MS/MS method was established using a mixed-mode anion exchange magnetic adsorbent for the simultaneous extraction of six functional B vitamins, including methylmalonic acid, riboflavin, pantothenic acid, 4-pyridoxic acid, folic acid, and 5-methyltetrahydrofolate.
View Article and Find Full Text PDFSimple and fast determination of tetrodotoxin in human plasma based on hydrophilic-interaction/ion-exchange mixed-mode solid phase extraction combined with liquid chromatography-tandem mass spectroscopy.
J Chromatogr A
November 2022
Central Laboratory, Shanghai Xuhui Central Hospital/Zhongshan-Xuhui Hospital, Fudan University, Shanghai, China; Shanghai Engineering Research Center of Phase I Clinical Research & Quality Consistency Evaluation for Drugs, Shanghai, China; Shanghai Institute of Clinical Mass Spectrometry, Shanghai,
Article Synopsis
- Developed a fast and sensitive LC-MS/MS method to measure tetrodotoxin (TTX) in human plasma using a specialized solid phase extraction plate.
- The Siphila i HILIX SPE plate was chosen for its superior extraction recovery (about 80%) and minimal matrix effects, attributed to its unique multi-carboxyl group functionality.
- The method demonstrated strong linearity and accuracy with a limit of quantification of 0.1 ng/mL and exhibited excellent performance against potential interferences in various plasma samples.
Development and validation of a ligand-binding assay for quantification of the F(ab') antivenom of Daboia russelii siamensis in human serum and its application to a phase I clinical study.
J Pharm Biomed Anal
April 2022
Shanghai Xuhui Central Hospital/ Zhongshan, Xuhui Hospital, Fudan University, Shanghai 200031, China; Shanghai Engineering Research Center of Phase I Clinical Research & Quality Consistency Evaluation for Drugs, Shanghai 200031, China. Electronic address:
Daboia russelii siamensis accounts for most of snakebite mortalities in China, yet, specific treatment against the venom toxins is absent in clinical practice. The F(ab') antivenom of Daboia russelii siamensis is manufactured and approved for the clinical trial in China. To satisfy the need for clinical pharmacokinetic research, this study aimed to develop a ligand binding assay (LBA) for the quantification of F(ab') antivenom of Daboia russelii siamensis in human serum.
View Article and Find Full Text PDFLiquid chromatography-tandem mass spectrometric assay for TPN171 in human plasma.
J Pharm Biomed Anal
November 2020
Shanghai Xuhui Central Hospital / Zhongshan- Xuhui Hospital, Fudan University, Shanghai 200031, China; Shanghai Engineering Research Center of Phase I Clinical Research & Quality Consistency Evaluation for Drugs, Shanghai 200031, China. Electronic address:
A simple, rapid and accurate method for quantitative analysis of a highly selective phosphodiesterase-5 inhibitor (PDE5), TPN171 by high performance liquid chromatography and tandem mass spectrometry in human plasma was proposed and validated successfully using D-TPN171 as internal standards (ISTD). An aliquot of 100 μL of plasma was mixed with internal standard and was precipitated with acetonitrile. Gradient elution was performed on a ACQUITY HSS T3 column (50 × 2.
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