Breaking barrier of binding buffer in colorimetric aptasensing of tetracycline in food fish using peroxidase mimic gold NanoZyme.

Tetracycline is extensively used in aquaculture as a therapeutic agent that needs to be monitored due to food safety concerns. Aptasensing has been revealed as a suitable diagnostic platform for tetracycline sensing in food matrix due to its quick, low cost and robust nature. But, the colorimetric aptasensing of tetracycline employing the peroxidase activity of gold nanoparticles (AuNPs) to 3,3,5,5-tetramethylbenzidine (TMB) was unsuitable until now owing to the aptamer-specific alkaline binding buffer.

Peroxidase mimic feroxyhyte (FeOOH) nanoparticles enabled highly specific colorimetric detection of arsenate in water and fish.

Arsenic poses a serious health risk to humans. Hence, a simple and robust analytical approach for monitoring arsenic levels in water and food matrixes is required. We present a simple and rapid approach for quantifying arsenate in water and fish using feroxyhyte (FeOOH) nanoparticles as a sensor probe.