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http://dx.doi.org/10.1002/alz.70685 | DOI Listing |
Alzheimers Dement
September 2025
Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Palo Alto, California, USA.
Alzheimers Dement
September 2025
UMR 1219 Bordeaux Population Health, Université de Bordeaux & INSERM, Bordeaux, France.
Sci Rep
February 2025
The Beijer Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University and SciLifeLab, Uppsala , Sweden.
Hundreds of loci have been robustly associated with obesity-related traits, but functional characterization of candidate genes remains a bottleneck. Aiming to systematically characterize candidate genes for a role in accumulation of lipids in adipocytes and other cardiometabolic traits, we developed a pipeline using CRISPR/Cas9, non-invasive, semi-automated fluorescence imaging and deep learning-based image analysis in live zebrafish larvae. Results from a dietary intervention show that 5 days of overfeeding is sufficient to increase the odds of lipid accumulation in adipocytes by 10 days post-fertilization (dpf, n = 275).
View Article and Find Full Text PDFJ Struct Biol
September 2023
State Key Laboratory of Protein and Plant Gene Research, Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, School of Life Sciences, Peking University, Beijing 100871, China; Changping Laboratory, Beijing 102206, China. Electronic address:
The structural studies of macromolecules in their physiological context, particularly in tissue, is constrained by the bottleneck of sample preparation. In this study, we present a practical pipeline for preparing multicellular samples for cryo-electron tomography. The pipeline comprises sample isolation, vitrification, and lift-out-based lamella preparation using commercially available instruments.
View Article and Find Full Text PDFEur J Hum Genet
December 2018
Braun School of Public Health, Hebrew University-Hadassah Medical Center, Jerusalem, Israel.
Genetic studies in isolated populations often increase power for identifying loci associated with complex diseases and traits. We present here the Kibbutzim Family Study (KFS), aimed at investigating the genetic basis of cardiometabolic traits in extended Israeli families characterized by long-term social stability and a homogeneous environment. Extensive information on cardiometabolic traits, as well as genome-wide genotypes, were collected on 901 individuals.
View Article and Find Full Text PDF