Multi-scale structure change and sugar derivatives of lotus seed cross-linked starch during dynamic simulated digestion in vitro.

The anti-digestive properties of cross-linked starches are essential for the development of low glycemic foods. Dynamic digestion modeling simulates the human digestive process more accurately and is an effective tool to study its anti-enzymatic mechanism. The structural evolution characteristics and the generation rules of sugar derivatives of lotus seed cross-linked starch with low, medium, high cross-linking degree (LS-2CS, LS-6CS, LS-12CS, respectively) were studied and compared during in vitro dynamic simulation digestive system. Studies of sugar derivatives found that LS-12CS showed an antidigestive advantage in simulating late gastrointestinal digestion. Similarly, there was no significant difference in the long-range and short-range ordered structures of LS-12CS after 60 min of simulated gastric digestion and 120 mins of simulated continuous gastrointestinal digestion. The spatial resistance it generates resisted the digestive enzymes' digestion of the long-branched chains, increased the average polymerization degree of branched starch, stabilized the amylose content, and promoted long-range ordered structure ordering and stabilization. This contributed to a better percentage of distarch monophosphate that effectively resisted starch digestion and better resistance to enzymatic degradation in dynamic digestion with LS-12CS. This work contributes to understanding the structure-digestive property relationship of LSCS and promotes its food application.