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Objectives: Establishing paediatric DRLs is challenging due to sparse data availability. The objective was to assess paediatric fluoroscopic dose levels in Dutch clinical practice, as current diagnostic reference levels (DRLs) need updating following the European Guidelines on DRLs for Paediatric Imaging (PiDRL).
Material And Methods: Air Kerma-area Product (KAP) values were retrospectively collected from paediatric patients (0-18 years) who underwent fluoroscopic procedures in nine Dutch hospitals between 01-01-2017 and 01-06-2021. The protocols included were: micturating cystourethrography (MCU), upper gastrointestinal (Upper GI) and lower gastrointestinal with contrast enema (Lower GI). In accordance with the PiDRL recommendations for sparse data, the 75th percentiles of the median KAP values per age group from each hospital were fitted using an exponential dose-age curve, resulting in an age-dependent DRL curve. DRL values were compared to Dutch, other European national and European DRLs.
Results: A total of 971 examinations were included. For MCU, the proposed DRL curve was lower than the Dutch DRLs. The proposed DRL curve for Upper GI was also lower than the UK DRLs. No DRL curve could be established for Lower GI due to limited data.
Conclusions: Paediatric fluoroscopic dose levels in this study are substantially lower than the current Dutch DRLs, indicating the need for new national Dutch DRLs for MCU. This study proposes using a DRL dose-age curve to update Dutch paediatric DRLs. Using the proposed curve method, more DRLs could be established than with the conventional method. The proposed DRL curves might serve as input for updating Dutch paediatric DRLs.
Key Points: Question Current Dutch diagnostic reference levels for paediatric fluoroscopy need updating, which is challenging due to sparse data availability. Findings Observed dose levels in this retrospective study indicate a potential decrease in Dutch diagnostic reference levels for paediatric fluoroscopy, using a dose-age curve method. Clinical relevance Updating Dutch paediatric fluoroscopic DRLs using a dose-age curve method allows for the establishment of DRLs in case of sparse data availability. This allows for further optimisation of radiation doses in the paediatric population.
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http://dx.doi.org/10.1007/s00330-025-11643-9 | DOI Listing |
J Appl Microbiol
September 2025
Graduate Institute of Medical Sciences, National Defense Medical University, Taipei City 114201, Taiwan (R.O.C.).
Aims: This study aims to develop and evaluate a rapid and high-multiplex pathogen detection method for clinical and food specimens to address the ongoing public health threat of foodborne infections and the limitations of conventional culture-based diagnostics.
Methods And Results: The foodborne bacteria (FBB) assay integrates multiplex PCR, T7 exonuclease hydrolysis, and a suspension bead array to simultaneously detect 16 genes from 13 major foodborne bacteria. Analytical performance was evaluated using reference strains, while diagnostic performance was assessed using clinical and food samples.
United European Gastroenterol J
September 2025
Gastroenterology and Endoscopy, IRCCS San Raffaele Hospital and Vita Salute San Raffaele University, Milan, Italy.
Background: Few data are available on the impact of primary sclerosing cholangitis (PSC) on inflammatory bowel disease (IBD).
Objective: We conducted a retrospective study using TriNetX to compare the outcomes of patients with IBD and those with concomitant IBD and PSC.
Methods: All patients with a confirmed diagnosis of Crohn's disease (CD), ulcerative colitis (UC), or indeterminate colitis with or without PSC were eligible.
Echocardiography
September 2025
Department of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Objectives: To explore the relationships between cardiac parameters and body composition indices, identifying predictors of subclinical cardiac systolic dysfunction.
Methods: Using anthropometric and serological parameters, echocardiography, and body composition analysis, this study evaluated metabolic profiles, cardiac remodeling patterns, and body composition characteristics in young adult obese patients, while quantifying the correlations between cardiac parameters and body composition indices. Subclinical left ventricular systolic dysfunction was defined as global longitudinal strain (GLS) < 18%.
Cell Mol Biol (Noisy-le-grand)
September 2025
Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud. Universidad de Guadalajara, Jalisco, México.
The objective of this study was to evaluate the concentration and integrity index of circulating cell-free DNA (ccf-DNA) as biomarkers for the detection and monitoring of minimal residual disease (MRD) in pediatric patients with B-cell acute lymphoblastic leukemia (B-ALL). Comparison with a validated methodology for the quantification of monoclonal rearrangements of the IGH gene was made. Peripheral blood and bone marrow samples were collected from 10 pediatric patients with B-ALL at diagnosis, remission, and maintenance phases.
View Article and Find Full Text PDFArch Microbiol
September 2025
Medical and Biological Computing Laboratory, School of Biosciences and Technology (SBST), Vellore Institute of Technology (VIT), Vellore, 632014, India.
Salmonella enterica serovar Typhi, the etiological agent of Typhoid fever, remains a critical public health concern associated with high morbidity in many developing countries. The widespread emergence of multidrug-resistant (MDR) Salmonella Typhi strains against the fluoroquinolone group of antibiotics, particularly ciprofloxacin, poses a significant global therapeutic challenge with underlying resistance due to mutations in quinolone-resistance determining region (QRDR) of gyrA gene, encoding DNA gyrase subunit A (GyrA). In pursuit of alternative therapeutic candidates, the present study was designed to evaluate ciprofloxacin analogues against prevalent GyrA mutations (S83F, D87G, and D87N) to overcome fluoroquinolone resistance through machine learning (ML)-based approach.
View Article and Find Full Text PDF