One-carbon Metabolism and Epigenetic Elements are Modulated by miR-1914-5p in an in Vitro Model of Steatosis.

Cell Biochem Biophys

Laboratório de Sinalização Celular e Expressão Gênica, Instituto de Biociências, Departamento de Biologia Geral e Aplicada, Universidade Estadual Paulista "Júlio de Mesquita Filho" - Campus Rio Claro, Rio Claro, SP, Brazil.

Published: September 2025


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12013-025-01881-5DOI Listing

Publication Analysis

Top Keywords

one-carbon metabolism
4
metabolism epigenetic
4
epigenetic elements
4
elements modulated
4
modulated mir-1914-5p
4
mir-1914-5p vitro
4
vitro model
4
model steatosis
4
one-carbon
1
epigenetic
1

Similar Publications

Metabolites as regulators of autoimmune diseases.

Front Immunol

September 2025

Department of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Immune cell metabolism is essential for regulating immune responses, including activation, differentiation, and function. Through glycolysis and oxidative phosphorylation (OXPHOS), metabolism supplies energy and key intermediates for cell growth and proliferation. Importantly, some metabolites generated during these processes act as signaling molecules that influence immune activity.

View Article and Find Full Text PDF

Serine hydroxymethyltransferase (SHMT) is a critical enzyme in the one-carbon (1C) metabolism pathway catalyzing the reversible conversion of L-Ser into Gly and concurrent transfer of 1C unit to tetrahydrofolate (THF) to give 5,10-methylene-THF (5,10-MTHF), which is used in the downstream syntheses of biomolecules critical for cell proliferation. The cellular 1C metabolism is hijacked by many cancer types to support cancer cell proliferation, making SHMT a promising target for the design and development of novel small-molecule antimetabolite chemotherapies. To advance structure-assisted drug design, knowledge of SHMT catalysis is crucial, but can only be fully realized when the atomic details of each reaction step governed by the acid-base catalysis are elucidated by visualizing active site hydrogen atoms.

View Article and Find Full Text PDF

One-carbon Metabolism and Epigenetic Elements are Modulated by miR-1914-5p in an in Vitro Model of Steatosis.

Cell Biochem Biophys

September 2025

Laboratório de Sinalização Celular e Expressão Gênica, Instituto de Biociências, Departamento de Biologia Geral e Aplicada, Universidade Estadual Paulista "Júlio de Mesquita Filho" - Campus Rio Claro, Rio Claro, SP, Brazil.

View Article and Find Full Text PDF

Objectives: Vitamin B12 plays a vital role in folate-mediated one-carbon metabolism (FOCM), a series of one-carbon transfer reactions that generate nucleotides (thymidylate (dTMP) and purines) and methionine. Inadequate levels of B12 impair FOCM, depressing de novo thymidylate (dTMP) synthesis, which in turn leads to uracil accumulation in DNA. This phenomenon has been well documented in nuclear DNA.

View Article and Find Full Text PDF

Photorespiration is the second largest carbon flux in most leaves and is integrated into metabolism broadly including one-carbon (C) metabolism. Photorespiratory intermediates such as serine and others may serve as sources of C units, but it is unclear to what degree this happens in vivo, whether altered photorespiration changes flux to C metabolism, and if so through which intermediates. To clarify these questions, we quantified carbon flux from photorespiration to C metabolism using CO labelling and isotopically non-stationary metabolic flux analysis in Arabidopsis thaliana under different O concentrations which modulate photorespiration.

View Article and Find Full Text PDF