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Pomegranate processing generates large quantities of pomace and mesocarp, yet their carbohydrate composition remains largely unexplored. Here, we applied comprehensive mass spectrometry-based profiling to characterize monosaccharides, oligosaccharides, and polysaccharides from these side streams. Fructose and glucose dominated the free sugar fraction in both tissues. We identified 77 oligosaccharides in pomace and 28 in mesocarp (3-8 monosaccharide units) composed of pentoses, hexoses, uronic acids, and deoxy-hexoses. LC-MS/MS quantification revealed distinct polysaccharide signatures: pomace was rich in glucose and xylose while mesocarp contained similar levels of xylose, arabinose and galacturonic acid. Compositional parallels between oligosaccharides and polysaccharides indicate the former arise from polysaccharide depolymerization. The diverse composition of oligosaccharides and polysaccharides underlines their promising prebiotic potential, positioning pomegranate byproducts as valuable functional ingredients and advancing their sustainable valorization within a circular bioeconomy.
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http://dx.doi.org/10.1016/j.foodchem.2025.146098 | DOI Listing |
Anim Sci J
September 2025
Department of Animal Science, Faculty of Agriculture, Ondokuz Mayis University, Atakum, Samsun, Türkiye.
This study aimed to evaluate the effect of some environment- and animal-based factors, such as body condition score (BCS) on 15-20 days before parturition, parity, and calving season on colostrum dry matter (DM), fat, solids-nonfat (SNF), protein, and lactose contents as well as colostrum and calf's serum immunoglobulin (IgG, IgA, and IgM), IGF-1, and lactoferrin concentrations in buffaloes. Therefore, the components of colostrum (at the first milking) and calf serum samples (at 24-48 h and 28 days after birth) from 86 Anatolian buffalo cows were analyzed by an infrared milk analyzer and ELISA test. The high BCS enhanced colostrum DM, fat, and IgG content; calf serum IgG concentration at 24-48 h, and lactoferrin at 28 days compared to low BCS.
View Article and Find Full Text PDFFood Res Int
November 2025
School of Food Science and Engineering, Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health, South China University of Technology, Guangzhou 510640, China; Sino-Singapore International Research Institute, Guangzhou 510555, China. Electronic address: zh
Breast milk is rich in bioactive proteins and oligosaccharides, including osteopontin (OPN) and 2'-fucosyllactose (2'-FL), which are believed to promote the growth of beneficial microbiota and regulate intestinal barrier function. In this study, fermentation substrates including DOPN (digested OPN fragment), 2'-FL and their combinations in varying proportions, were prepared through in vitro gastrointestinal digestion, dialysis and freeze-drying. Changes in gas production, organic acid levels, ammonia N concentration and bacterial population abundance were studied using an in vitro batch fermentation model, with feces inocula from healthy infants.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, China. Electronic address:
This research explored the effects of ginseng residue oligosaccharides (GRO-N) and ginseng polysaccharides (GP-N) on alleviating allergic rhinitis (AR). In a rat model induced by ovalbumin (OVA), both high doses of GRO-N (GRO-N-H) and GP-N (GP-N-H) significantly decreased the frequency of sneezing and rubbing behaviors in AR-affected rats. Histopathological evaluations and cytokine analyses revealed that GRO-N-H and GP-N-H notably lowered the count of goblet cells and reduced inflammatory cytokine levels in these rats.
View Article and Find Full Text PDFPLoS One
September 2025
Division of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, Japan.
Zygotes are used to create genetically modified animals by electroporation using the CRISPR-Cas9 system. Such zygotes in rats are obtained from superovulated female rats after mating. Recently, we reported that in vivo-fertilized zygotes had higher cryotolerance and developmental ability than in vitro-fertilized zygotes in Sprague Dawley (SD) and Fischer 344 rats.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Academy of Advanced Carbon Conversion Technology, Huaqiao University, Xiamen, 361021, China; Fujian Provincial Key Laboratory of Biomass Low-Carbon Conversion, Huaqiao University, Xiamen, 361021, China; College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China. Electronic address: l
Over recent decades, the indiscriminate use of antibiotics in animal production to enhance product quality and maximize economic returns has raised critical concerns. However, antibiotic misuse has led to the development of antimicrobial resistance in livestock and poses substantial health risks to humans through drug residue accumulation. In response, nations globally have progressively implemented bans on antibiotic inclusion in animal nutrition, redirecting scientific attention toward antibiotic-free feed additives that maintain or enhance animal health performance.
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