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The use of polyols as sucrose-replacer in chocolate formulation has increased in the last years because of the high occurrence of the metabolic syndrome. Furthermore, chocolate is a qualified vehicle of functional ingredients such as probiotics that improve health in people with diabetes. The first objective of this study was to evaluate the optimal formulation of sucrose-free dark chocolate (SFDC) using a mixture design. The impact of polyols (maltitol, lactitol, and xylitol) as sucrose replacers on the main quality properties of SFDC was determined. In the second stage, was incorporated in the optimized formulation of SFDC as a probiotic product. Chocolates were stored at two temperatures (4 °C and 20 °C) for 180 days and the viability of probiotics was determined. The optimization data revealed that using 29.49 % maltitol, 51.46 % lactitol, and 19.05 % xylitol ended in the optimum SFDC with the highest desirability. The number of probiotics were retained at the functional amount after maintenance for 6 months. The highest probiotic viability was determined in the chocolates stored at 4 °C. The SFDCs possessing probiotics showed to be a promising sucrose-free functional chocolate.
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http://dx.doi.org/10.1016/j.crfs.2025.101174 | DOI Listing |
J Food Sci Technol
October 2025
Department of Food Science, Tunghai University, Taichung City, 40704 Taiwan.
Chocolate is often used in 3D food printing, however in 3D food printing cold extrusion systems, chocolate often faces the issue of temperature-induced clumping. To address this texture alteration, the method of adding oleogels is employed. This study examines the impact of monoglycerides (MAG), sucrose fatty acid ester (SE) and hydroxypropyl methylcellulose (HPMC) oleogels on the thermal and textural properties of 3D printed white and dark chocolates.
View Article and Find Full Text PDFCurr Eye Res
September 2025
National Engineering Research Center of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, P.R. China.
Purpose: To explore the effects of dark chocolate during relaxation and near work on choroidal thickness and blood flow parameters in myopic patients.
Methods: Thirty-one myopic subjects were recruited for this self-controlled study. Participants need to complete two experiments.
Curr Res Food Sci
August 2025
Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
The use of polyols as sucrose-replacer in chocolate formulation has increased in the last years because of the high occurrence of the metabolic syndrome. Furthermore, chocolate is a qualified vehicle of functional ingredients such as probiotics that improve health in people with diabetes. The first objective of this study was to evaluate the optimal formulation of sucrose-free dark chocolate (SFDC) using a mixture design.
View Article and Find Full Text PDFJ Agric Food Chem
September 2025
Chair of Special Food Chemistry, Technische Universität Dresden, D-01062 Dresden, Germany.
During chocolate production, thermal processes such as roasting and conching promote nonenzymatic browning reactions such as the Maillard reaction and caramelization. In the present work, the MRPs furosine, 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 5-hydroxymethylfurfural (HMF), -ε-fructosyllysine, -ε-lactulosyllysine, -ε-carboxymethyllysine (CML), -ε-carboxyethyllysine (CEL), pyrraline, methylglyoxal-derived hydroimidazolone 1 (MG-H1), formyline, maltosine, and rhamnolysine were quantitated in 4 filled, 12 dark, 11 milk, and 4 white chocolate samples. The predominant MRP in filled chocolates was -ε-fructosyllysine (up to 2662 mg/kg of chocolate), whereas in milk chocolates, it was -ε-lactulosyllysine (up to 883 mg/kg of chocolate).
View Article and Find Full Text PDFSports (Basel)
August 2025
Department of Human Sciences and Promotion of the Quality of Life, San Raffaele Open University, Via di Val Cannuta 247, 00166 Rome, Italy.
Background: The relationship between sports participation and food preferences in adults, as well as the influence of gender, is still unclear.
Objective: The objective of this study was to investigate the association between sports participation and individual food preferences and to explore potential gender differences among sports participants in a large group of Italian adults.
Methods: This cross-sectional study involved 2665 adults (aged ≥ 18 years) who lead normal lives and underwent a routine lifestyle and dietary assessment at a clinical centre specialising in nutrition, metabolic health, and lifestyle counselling in Rome.